Journal of Microbiology and Biotechnology

  • 제28권12호
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  • Pages.2029-2035
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  • 2018
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Biochemical Characterization of Alkaliphilic Cyclodextran Glucanotransferase from an Alkaliphilic Bacterium, Paenibacillus daejeonensis

  • Yang, So-Jin (Department of Food Science and Technology and BK21 Plus Program, Chonnam National University) ;
  • Ko, Jin-A (Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Hae-Soo (Department of Food Science and Technology and BK21 Plus Program, Chonnam National University) ;
  • Jo, Min-Ho (Department of Food Science and Technology and BK21 Plus Program, Chonnam National University) ;
  • Lee, Ha-Nul (Department of Food Science and Technology and BK21 Plus Program, Chonnam National University) ;
  • Park, Bo-Ram (Department of Agrofood Resources National Academy of Agricultural Science, RDA) ;
  • Kim, Young-Min (Department of Food Science and Technology and BK21 Plus Program, Chonnam National University)
  • 투고 : 2018.10.04
  • 심사 : 2018.10.25
  • 발행 : 2018.12.28
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초록

Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a single protein band of 111 kDa. PDCITase showed optimum activity at pH 8.0 and retained 100% of activity within a broad pH range (7.0-11.5) after 18 h, indicating alkaliphilic or alkalistable CITase properties. In addition, PDCITase produced CI-7 to CI-17, CI-18, and CI-19, which are relatively large cycloisomaltooligosaccharides yet to be reported. Therefore, these large cycloisomaltooligosaccharides can be applied to the improvement of water solubility of pharmaceutical biomaterials.

키워드

참고문헌

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피인용 문헌

  1. Thermostable CITase from Thermoanaerobacter thermocopriae shows negative cooperativity vol.41, pp.4, 2018, https://doi.org/10.1007/s10529-019-02666-6
  2. Carboxy-Terminal Region of a Thermostable CITase from Thermoanaerobacter thermocopriae Has the Ability to Produce Long Isomaltooligosaccharides vol.29, pp.12, 2018, https://doi.org/10.4014/jmb.1910.10022