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http://dx.doi.org/10.4014/jmb.1810.10007

Biochemical Characterization of Alkaliphilic Cyclodextran Glucanotransferase from an Alkaliphilic Bacterium, Paenibacillus daejeonensis  

Yang, So-Jin (Department of Food Science and Technology and BK21 Plus Program, Chonnam National University)
Ko, Jin-A (Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Kim, Hae-Soo (Department of Food Science and Technology and BK21 Plus Program, Chonnam National University)
Jo, Min-Ho (Department of Food Science and Technology and BK21 Plus Program, Chonnam National University)
Lee, Ha-Nul (Department of Food Science and Technology and BK21 Plus Program, Chonnam National University)
Park, Bo-Ram (Department of Agrofood Resources National Academy of Agricultural Science, RDA)
Kim, Young-Min (Department of Food Science and Technology and BK21 Plus Program, Chonnam National University)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.12, 2018 , pp. 2029-2035 More about this Journal
Abstract
Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a single protein band of 111 kDa. PDCITase showed optimum activity at pH 8.0 and retained 100% of activity within a broad pH range (7.0-11.5) after 18 h, indicating alkaliphilic or alkalistable CITase properties. In addition, PDCITase produced CI-7 to CI-17, CI-18, and CI-19, which are relatively large cycloisomaltooligosaccharides yet to be reported. Therefore, these large cycloisomaltooligosaccharides can be applied to the improvement of water solubility of pharmaceutical biomaterials.
Keywords
Cyclodextran; cycloisomaltooligosaccharide glucanotransferase; Paenibacillus daejeonensis; glycoside hydrolase family 66;
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