Fig. 2. Stability of ECP33 and NOECP91 at (A) 65 and (B)70℃.
Fig. 3. Stability of ECP33 and NOECP91 under various pH (A) and ethanol (B) conditions for 30 min and 1 h.
Fig. 4. Stability of ECP33 and NOECP91 at (A) 50 ppm of NaClO and (B) 100 ppm of NaClO.
Fig. 5. Growth inhibition using bacteriophage for E. coli NCCP 13930 (O157:H7) and E. coli NCCP 13937 (O103).
Fig. 1. Transmission Electron Microscopy micrographs of (A) ECP33 and (B) NOECP91.
Table 1. Host spectrum of ECP33 and NOECP91 isolated for shiga-toxin producing E .coli
References
- Ackermann HW. Bacteriophage observations and evolution. Res. Microbiol. 154: 245-251 (2003) https://doi.org/10.1016/S0923-2508(03)00067-6
- Brooks JT, Sowers EG, Wells JG, Greene KD, Griffin PM, Hoekstra RM, Strockbine NA. Non-O157 shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J. Infect. Dis. 192: 1422-1429 (2005) https://doi.org/10.1086/466536
- Brussow H. Phage therapy: The Escherichia coli experience. Microbiol. 151: 2133-2140 (2005) https://doi.org/10.1099/mic.0.27849-0
- Buzrul S, Ozturk P, Alpas H, Akcelik M. Thermal and chemical inactivation of lactococcal bacteriophages. LWT-Food Sci. Technol. 40: 1671-1677 (2007) https://doi.org/10.1016/j.lwt.2007.01.002
- Dini C, Urraza PJ. Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and shiga toxin-producing E. coli (EHEC and ETEC) in cattle. J. Appl. Microbiol. 109: 873-887 (2010) https://doi.org/10.1111/j.1365-2672.2010.04714.x
- Frank C, Werber D, Cramer JP, Askar M, Faber M, Heiden M, Bernard H, Fruth A, Prager R, Spode A, Wadl M. Epidemic profile of shiga-toxin-producing Escherichia coli O104: H4 outbreak in Germany. New Eng. J. Med. 365: 1771-1780 (2011) https://doi.org/10.1056/NEJMoa1106483
- Gould LH, Mody RK, Ong KL, Clogher P, Cronquist AB, Garman KN, Lathrop S, Medus C, Spina NL, Webb TH, White PL. Increased recognition of non-O157 shiga toxin-producing Escherichia coli infections in the United States during 2000-2010: Epidemiologic features and comparison with E. coli O157 infections. Foodborne Pathog. Dis. 10: 453-460 (2013) https://doi.org/10.1089/fpd.2012.1401
- Guglielmotti DM, Mercanti DJ, Reinheimer JA, Quiberoni Adel L. Efficiency of physical and chemical treatments on the inactivation of dairy bacteriophages. Front. Microbiol. 2: 282 (2011)
- Hendrix RW. Bacteriophage genomics. Curr. Opin. Microbiol. 6: 506-511 (2003) https://doi.org/10.1016/j.mib.2003.09.004
- Joczyk E, Kak M, Mizybrodzki R, Gorski A. The influence of external factors on bacteriophages. Folia Microbiol. 56: 191-200 (2011) https://doi.org/10.1007/s12223-011-0039-8
- Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat. Rev. Microbiol 2: 123-140 (2004) https://doi.org/10.1038/nrmicro818
- Karch H, Denamur E, Dobrindt U, Finlay BB, Hengge R, Johannes L, Ron EZ, Tonjum T, Sansonetti PJ, Vicente M. The enemy within us: Lessons from the 2011 European Escherichia coli O104: H4 outbreak. EMBO Mol. Med. 4: 841-848 (2012) https://doi.org/10.1002/emmm.201201662
- Khakhria R, Duck D, Lior H. Extended phage-typing scheme for Escherichia coli 0157: H7. Epidemiol. Infect. 105: 511-520 (1990) https://doi.org/10.1017/S0950268800048135
- Kim JY. Characterization of bacteriophage for enteroheamorrhagic E. coli and application as sanitizer. MS thesis, Gachon University, Sungnam, Korea (2013)
- Kim EJ, Chang HJ, Kwak SJ, Park JH. Virulence factors and stability of coliphages specific to Escherichia coli O157:H7 and to various E. coli infection. J. Microbiol. Biotechnol. 26: 2060-2065 (2016a) https://doi.org/10.4014/jmb.1609.09039
- Kim MJ, Kim SH, Kim TS, Kee HY, Seo JJ, Kim ES, Park JT, Chung JK, Lee J. Identification of shiga toxin-producing E. coli isolated from diarrhea patients and cattle in Gwangju area, Korea. J. Bacteriol. Virol. 39: 29-39 (2009) https://doi.org/10.4167/jbv.2009.39.1.29
- Kim EJ, Lee H, Lee JH, Ryu S, Park JH. Morphological features and lipopolysaccharide attachment of coliphages specific to Escherichia coli O157: H7 and to a broad range of E. coli hosts. Appl. Biol. Chem. 59: 109-116 (2016b) https://doi.org/10.1007/s13765-015-0130-y
- Law D. Virulence factors of Escherichia coli O157 and other shiga toxin-producing E. coli. J. Appl. Microbiol. 88: 729-745 (2000) https://doi.org/10.1046/j.1365-2672.2000.01031.x
- Lee YD, Kim JY, Park JH. Characteristics of coliphage ECP4 and potential use as a sanitizing agent for biocontrol of Escherichia coli O157:H7. Food Control 34: 255-260 (2013) https://doi.org/10.1016/j.foodcont.2013.04.043
- Lee YD, Park JH. Isolation and characterization of temperate phages in Enterococcus faecium from Sprouts. Korean J. Food Sci. Technol. 46: 323-327 (2014) https://doi.org/10.9721/KJFST.2014.46.3.323
- Lee YD, Park JH. Characterization and application of phages isolated from sewage for reduction of Escherichia coli O157:H7 in biofilm. LWT-Food Sci. Technol. 60: 571-577 (2015) https://doi.org/10.1016/j.lwt.2014.09.017
- Lienemann T, Kyyhkynen A, Halkilahti J, Haukka K, Siitonen A. Characterization of Salmonella Typhimurium isolates from domestically acquired infections in Finland by phage typing, antimicrobial susceptibility testing, PFGE and MLVA. BMC Microbiol. 15: 131 (2015) https://doi.org/10.1186/s12866-015-0467-8
- Majowicz SE, Scallan E, Jones-Bitton A, Sargeant JM, Stapleton J, Angulo FJ, Yeung DH, Kirk MD. Global incidence of human shiga toxin-producing Escherichia coli infections and deaths: A systematic review and knowledge synthesis. Foodborne Pathog. Dis. 11: 447-455 (2014) https://doi.org/10.1089/fpd.2013.1704
- Marks T, Sharp R. Bacteriophages and biotechnology: A review. J. Chem. Tech. Biot. 75: 6-17 (2000) https://doi.org/10.1002/(SICI)1097-4660(200001)75:1<6::AID-JCTB157>3.0.CO;2-A
- Mathusa EC, Chen Y, Enache E, Hontz L. Non-O157 shiga toxinproducing Escherichia coli in foods. J. Food Protect. 73: 1721-1736 (2010) https://doi.org/10.4315/0362-028X-73.9.1721
- Minakhin L, Goel M, Berdygulova Z, Ramanculov E, Florens L, Glazko G, Karamychev VN, Slesarev AI, Kozyavkin SA, Khromov I, Ackermann HW. Genome comparison and proteomic characterization of Thermus thermophilus bacteriophages P23-45 and P74-26: Siphoviruses with triplex-forming sequences and the longest known tails. J. Mol. Biol. 378: 468-480 (2008) https://doi.org/10.1016/j.jmb.2008.02.018
- Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201 (1998) https://doi.org/10.1128/CMR.11.1.142
- Nakao H, Kataoka C, Kiyokawa N, Fujimoto J, Yamasaki S, Takeda T. Monoclonal antibody to shiga toxin 1, which blocks receptor binding and neutralizes cytotoxicity. Microbiol. Immunol. 46: 777-780 (2002) https://doi.org/10.1111/j.1348-0421.2002.tb02764.x
- O'Flynn G, Ross RP, Fitzgerald GF, Coffey A. Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7. Appl. Environ. Microbiol. 70: 3417-3424 (2004) https://doi.org/10.1128/AEM.70.6.3417-3424.2004
- Oliveira A, Sillankorva S, Quinta R, Henriques A, Sereno R, Azeredo J. Isolation and characterization of bacteriophages for avian pathogenic E. coli strains. J. Appl. Microbiol. 106: 1919-1927 (2009) https://doi.org/10.1111/j.1365-2672.2009.04145.x
- Raya RR, Varey P, Oot RA, Dyen MR, Callaway TR, Edrington TS, Kutter EM, Brabban AD. Isolation and characterization of a new T-even bacteriophage, CEV1, and determination of its potential to reduce Escherichia coli O157: H7 levels in sheep. Appl. Environ. Microbiol. 72: 6405-6410 (2006) https://doi.org/10.1128/AEM.03011-05
- Sanekata T, Fukuda T, Miura T, Morino H, Lee C, Maeda K, Araki K, Otake T, Kawahata T, Shibata T. Evaluation of the antiviral activity of chlorine dioxide and sodium hypochlorite against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus. Biocontrol Sci. 15: 45-49 (2010) https://doi.org/10.4265/bio.15.45
- Santos TMA, Gilbert RO, Caixeta LS, Machado VS, Teixeira LM, Bicalho RC. Susceptibility of Escherichia coli isolated from uteri of postpartum dairy cows to antibiotic and environmental bacteriophages. Part II: In vitro antimicrobial activity evaluation of a bacteriophage cocktail and several antibiotics. J. Dairy Sci. 93: 105-114 (2010) https://doi.org/10.3168/jds.2009-2299
- Sass P, Bierbaum G. Lytic activity of recombinant bacteriophage 11 and 12 endolysins on whole cells and biofilms of Staphylococcus aureus. Appl. Environ. Microbiol. 73: 347-352 (2007) https://doi.org/10.1128/AEM.01616-06
- Smith JL, Fratamico PM, Gunther IV NW. Shiga toxin-producing Escherichia coli. Adv. Appl. Microbiol. 86: 145-197 (2014)
- Sulakvelidze A, Alavidze Z, Morris JG. Bacteriophage therapy. Antimicrob. Agents Ch. 45: 649-659 (2001) https://doi.org/10.1128/AAC.45.3.649-659.2001
- Tzipilevich E, Habusha M, Ben-Yehuda S. Acquisition of phage sensitivity by bacteria through exchange of phage receptors. Cell 168: 186-199 (2017) https://doi.org/10.1016/j.cell.2016.12.003