Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Acute Toxicity Assessment in Zebrafish Danio rerio of Arsenic-rich Extracts from Three Species of Seaweeds

제브라피쉬(Danio rerio)를 이용한 비소 고함류 3종 해조류 추출물의 급성 독성평가

  • Yang, Hye-Won (Department of Marine Life Science, Jeju National University) ;
  • Kim, Eun-A (Jeju Environment Research Section, Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology) ;
  • Kim, Seo-Young (Department of Marine Life Science, Jeju National University) ;
  • Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
  • 양혜원 (제주대학교 해양생명과학과) ;
  • 김은아 (한국해양과학기술원 제주국제해양과학연구.지원센터 제주특성연구실) ;
  • 김서영 (제주대학교 해양생명과학과) ;
  • 전유진 (제주대학교 해양생명과학과)
  • Received : 2017.12.19
  • Accepted : 2018.01.24
  • Published : 2018.02.28
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Abstract

Seaweeds are composed of a variety of bioactive substances, including polysaccharides, pigments, minerals, peptides, and polyphenols. Among these substances, the arsenic content of seaweeds has been a significant cause for concern. The present study evaluated the toxicity of arsenic from three species of seaweed using a zebrafish Danio rerio model. The arsenic-rich extracts were obtained from Ecklonia cava (ECAE), Undaria pinnatifida (UPAE) and Hizikia fusiformis (HFAE) using a solvent of 50% methanol and 1% $HNO_3$. We investigated the toxicity of the arsenic-rich extracts in zebrafish embryos through survival rate, heart rate, yolk sac edema size, cell death, reactive oxygen species (ROS) production and real-time polymerase chain reaction (PCR). The hepatotoxicity of arsenic-rich extracts was assessed in the liver of adult zebrafish through real-time PCR and histopathology. The survival rates of embryos and adult zebrafish showed no significant changes at any concentration. At 100 ppm, embryos did not exhibit significant differences in heart rate, yolk sac edema size, cell death or ROS production. In addition, apoptosis-related genes in larvae and liver tissue were unaffected by treatment with arsenic-rich extracts. These data will help clarify that developmental changes, hepatic oxidative stress, and apoptosis are not associated with toxicity from arsenic-rich seaweed extracts in a zebrafish model.

Keywords

References

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