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http://dx.doi.org/10.5657/KFAS.2018.0031

Acute Toxicity Assessment in Zebrafish Danio rerio of Arsenic-rich Extracts from Three Species of Seaweeds  

Yang, Hye-Won (Department of Marine Life Science, Jeju National University)
Kim, Eun-A (Jeju Environment Research Section, Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology)
Kim, Seo-Young (Department of Marine Life Science, Jeju National University)
Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.51, no.1, 2018 , pp. 31-41 More about this Journal
Abstract
Seaweeds are composed of a variety of bioactive substances, including polysaccharides, pigments, minerals, peptides, and polyphenols. Among these substances, the arsenic content of seaweeds has been a significant cause for concern. The present study evaluated the toxicity of arsenic from three species of seaweed using a zebrafish Danio rerio model. The arsenic-rich extracts were obtained from Ecklonia cava (ECAE), Undaria pinnatifida (UPAE) and Hizikia fusiformis (HFAE) using a solvent of 50% methanol and 1% $HNO_3$. We investigated the toxicity of the arsenic-rich extracts in zebrafish embryos through survival rate, heart rate, yolk sac edema size, cell death, reactive oxygen species (ROS) production and real-time polymerase chain reaction (PCR). The hepatotoxicity of arsenic-rich extracts was assessed in the liver of adult zebrafish through real-time PCR and histopathology. The survival rates of embryos and adult zebrafish showed no significant changes at any concentration. At 100 ppm, embryos did not exhibit significant differences in heart rate, yolk sac edema size, cell death or ROS production. In addition, apoptosis-related genes in larvae and liver tissue were unaffected by treatment with arsenic-rich extracts. These data will help clarify that developmental changes, hepatic oxidative stress, and apoptosis are not associated with toxicity from arsenic-rich seaweed extracts in a zebrafish model.
Keywords
Zebrafish; Arsenic-rich extract; In vivo model; Seaweed; Toxicity;
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1 Abdel-Warith AA, Younis EM, Al-Asgah NA and Wanbi OM. 2011. Effect of zinc toxicity on liver histology of Nile tilapia, Oreochromis niloticus. Sci Res Essays 6, 3760-3769. http://dx.doi.org/10.5897/SRE11.883.   DOI
2 ATSDR. 2007. Toxicological profile for arsenic. Agency for toxic substances and disease registry, Division of toxicology, Atlanta, GA, U.S.A.
3 Braunbeck T and Lammer E. 2006. Fish embryo toxicity assays, German Federal Environment Agency, Dessau, Germany, 1-298.
4 Choi H, Park SK, Kim DS and Kim M. 2011. Determination of 6 arsenic present in seaweed by solvent extraction, cleanup, and LC-ICP/MS. Food Sci Biotechnol 20, 39-44. http://dx.doi.org/10.1007/s10068-011-0006-9.   DOI
5 Deng J, Yu L, Liu C, Yu K, Shi X, Yeung LWY, Lam PKS, Wu RSS and Zhou B. 2009. Hexabromocyclododecane-induced developmental toxicity and apoptosis in zebrafish embryos. Aquat Toxicol 93, 29-36. https://dx.doi.org/10.1016/j.aquatox.2009.03.001.   DOI
6 Devesa V, Martinez A, Suner MA, Benito V, Velez D and Montoro R. 2001. Kinetic study of transformations of arsenic species during heat treatment. J Agric Food Chem 49, 2267-2271. https://dx.doi.org/10.1021/jf001328e.   DOI
7 Edmonds JS, Shibata Y, Francesconi KA, Rippingale RJ and Morita M. 1997. Arsenic transformations in short marine food chains studied by HPLC.ICP MS. Appl Organomet Chem 11, 281-287. https://dx.doi.org/10.1002/(SICI)1099-0739(199704)11:4<281::AID-AOC581>3.0.CO;2-S.   DOI
8 EFSA GMO Panel Working Group on Animal Feeding Trials. 2008. Safety and nutritional assessment of GM plants and derived food and feed: the role of animal feeding trials. Food Chem Toxicol 46, S2-S70. http://dx.doi.org/10.1016/j.fct.2008.02.008.   DOI
9 Fan TJ, Han LH, Cong RS and Liang J. 2005. Caspase family proteases and apoptosis. Acta Biochim Biophys Sin 37, 719-727. https://dx.doi.org/10.1111/j.1745-7270.2005.00108.x.   DOI
10 Fraysse B, Mons R and Garric J. 2006. Development of a zebrafish 4-day embryo-larval bioassay to assess toxicity of chemicals. Ecotoxicol Environ Saf 63, 253-267. https://dx.doi.org/10.1016/j.ecoenv.2004.10.015.   DOI
11 Henn K and Braunbeck T. 2011. Dechorionation as a tool to improve the fish embryo toxicity test (FET) with the zebrafish (Danio rerio). Com Biochem Physiol C Toxicol Pharmacol 153, 91-98. https://dx.doi.org/10.1016/j.cbpc.2010.09.003.   DOI
12 Kim AJ, Kim SY, Lee WC and Park M. 1998. Contents of arsenic in some fisheries caught in western coast. J Food Hyg Saf 13, 201-205.
13 Hill AJ, Teraoka H, Heideman W and Peterson RE. 2005. Zebrafish as a model vertebrate for investigating chemical toxicity. Toxicol Sci 86, 6-19. https://dx.doi.org/10.1093/toxsci/kfi110.   DOI
14 Hutchinson TH. 2002. Impacts of endocrine disrupters on fish development: opportunities for adapting OECD Test Guideline 210. Environ Sci 9, 439-450.
15 Jeoung IG, Ha KS and Choi JD. 2009. Arsenic speciation and risk assesment of Hijiki (Hizikia fusiforme) by HPLC-ICP-MS. Korean J Food Sci Technol 41, 1-6.
16 Kim EA, Lee SH, Ko CI, Cha SH, Kang MC, Kang SM, Ko SC, Lee WW, Ko JY and Lee JH. 2014. Protective effect of fucoidan against AAPH-induced oxidative stress in zebrafish model. Carbohydr Polym 102, 185-191. https://dx.doi.org/10.1016/j.carbpol.2013.11.022.   DOI
17 Kim KS. 2005. Heavy metal survey of agricultural products in jeollanambuk province. Korea Food & Drug Administration, Seoul, Korea.
18 Kim KS. 2007. Development of analysis method on arsenic chemicals in sea food. Korea Food & Drug Administration, Seoul, Korea.
19 Kimmel CB, Ballard WW, Kimmel SR, Ullmann B and Schilling TF. 1995. Stages of embryonic development of the zebrafish. Dev Dyn 203, 253-310. https://dx.doi.org/10.1002/aja.1002030302.   DOI
20 Langheinrich U, Hennen E, Stott G and Vacun G. 2002. Zebrafish as a model organism for the identification and characterization of drugs and genes affecting p53 signaling. Curr Biol 12, 2023-2028. https://dx.doi.org/10.1016/S0960-9822(02)01319-2   DOI
21 Sun L, Xin L, Peng Z, Jin R, Jin Y, Qian H and Fu Z. 2014. Toxicity and enantiospecific differences of two $\beta$-blockers, propranolol and metoprolol, in the embryos and larvae of zebrafish (Danio rerio). Environ Toxicol 29, 1367-1378. https://dx.doi.org/10.1002/tox.21867.   DOI
22 Sheikh MS and Fornace AJ. 2000. Role of p53 family members in apoptosis. J Cell Physiol 182, 171-181. https://dx.doi.org/10.1002/(SICI)1097-4652(200002)182:2<171::AID-JCP5>3.0.CO;2-3.   DOI
23 Snell C, Bernheim A, Berge JB, Kuntz M, Pascal G, Paris A and Ricroch AE. 2012. Assessment of the health impact of GM plant diets in long-term and multigenerational animal feeding trials: a literature review. Food Chem Toxicol 50, 1134-1148. https://dx.doi.org/10.1016/j.fct.2011.11.048.   DOI
24 Soma S, Choi JW, Lee MK, Kim YM, Kim IH and Nam TJ. 2014. Protective effects of Pyropia yezoensis glycoprotein against ethanol-induced chronic gastric injury in the rat. Korean J Fish Aquat Sci 47, 765-769. http://dx.doi.org/10.5657/KFAS.2014.0765.   DOI
25 Valko M, Morris H and Cronin MTD. 2005. Metals, toxicity and oxidative stress. Curr Med Chem 12, 1161-1208. https://dx.doi.org/10.2174/0929867053764635   DOI
26 Wang H, Che B, Duan A, Mao J, Dahlgre RA, Zhang M, Zhan H, Zeng A and Wang X. 2014. Toxicity evaluation of $\beta$-diketone antibiotics on the development of embryo-larval zebrafish (Danio rerio). Environ Toxicol 29, 1134-1146. https://dx.doi.org/10.1002/tox.21843.   DOI
27 Yamashita M. 2003. Apoptosis in zebrafish development. Comp Biochem Physiol B Biochem Mol Biol 136, 731-742. https://doi.org/10.1016/j.cbpc.2003.08.013.   DOI
28 Lee HS, Cho YH, Park SO, Kye SH, Kim BH, Hahm TS, Kim M, Lee JO and Kim CI. 2006. Dietary exposure of the Korean population to arsenic, cadmium, lead and mercury. J Food Compost Anal 19, S31-S37. https://dx.doi.org/10.1016/j.jfca.2005.10.006.   DOI
29 Launay S, Hermine O, Fontenay M, Kroemer G, Solary E and Garrido C. 2005. Vital functions for lethal caspases. Oncogene 24, 5137-5148. https://dx.doi.org/10.1038/sj.onc.1208524.   DOI
30 Lee SK, Yang JY, Kim KW, Lee SY, Kwon TJ and Yoo YC. 2003. Distribution of arsenic in korean human tissues. J Environ Toxicol 18, 101-109.
31 McSheehy S, Szpunar J, Morabito R and Quevauviller P. 2003. The speciation of arsenic in biological tissues and the certification of reference materials for quality control. TrAC Trends Anal Chem 22, 191-209. https://dx.doi.org/10.1016/S0165-9936(03)00404-7.   DOI
32 OECD (Organisation for Economic Co-Operation and Development). 1981. OCED guidelines for the testing of chemicals, section 4: health effects. OECD, Paris, France. https://dx.doi.org/10.1787/20745788.   DOI
33 Ohinata Y, Miller JM and Schacht J. 2003. Protection from noise-induced lipid peroxidation and hair cell loss in the cochlea. Brain Res 966, 265-273. https://dx.doi.org/10.1016/S0006-8993(02)04205-1.   DOI
34 Palk MK, Kim WI, Yoo JH, Kim JK, Kim MJ, Im GJ, Hong MK, and Om AS. 2010. Trends of arsenic maximum levels on agricultural commodities and processed agricultural products. J Fd Hyg Safety 25, 16-23.
35 Ryu KY, Shim SL, Hwang IM, Jung MS, Jun SM, Seo HY, Park JS, Kim HY, Om AS, Park KS, and Kim KS. 2009. Arsenic Speciation and Risk Assesment of Hijiki (Hizikia fusiforme) by HPLC-ICP-MS. Korean J Food Sci Technol 41, 1-6.