Korean Chemical Engineering Research

  • 제56권6호
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  • Pages.826-831
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  • 2018
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  • 0304-128X(pISSN)
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  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
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미세 성형 방법을 이용한 형광 표지된 이중 분획 입자의 제조

Fabrication of Fluorescent Labeled Bi-compartmental Particles via the Micromolding Method

  • 심규락 (충남대학교 응용화학공학과) ;
  • 정성근 (충남대학교 응용화학공학과) ;
  • 홍우경 (충남대학교 응용화학공학과) ;
  • 강경구 (충남대학교 응용화학공학과) ;
  • 이창수 (충남대학교 응용화학공학과)
  • Shim, Gyurak (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Jeong, Seong-Geun (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Hong, Woogyeong (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Kang, Koung-Ku (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Lee, Chang-Soo (Department of Chemical Engineering and Applied Chemistry, Chungnam National University)
  • 투고 : 2018.09.14
  • 심사 : 2018.10.04
  • 발행 : 2018.12.01
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초록

본 연구는 다중 형광이 표지된 이중 분획 입자의 제조에 관한 것이다. 입자 내에서 형광 발현을 분획화하기 위하여, 형광의 여기 및 방출 스펙트럼의 중첩이 적은 두가지의 형광 염료를 선정한다. 또한, 형광 안정성을 확보하기 위하여 선정된 형광 염료는 입자를 구성하는 소재와 함께 가교될 수 있도록 분자 내에 아크릴레이트(acrylate) 작용기를 포함한다. 공초점 현미경 촬영을 통하여 선정된 형광 물질을 이용하여 제조된 입자에서 강한 형광 발현 및 형광의 분획화를 확인하였다. 더 나아가 4주 동안 형광 발현 및 세기를 측정하여 장기간의 형광 안정성을 검증하였다. 본 연구에서 제조된 다중 형광 표지된 이중 분획 입자는 다중 표적형 약물 전달 체계, 3차원 브라운 운동의 해석 연구, 3차원의 복잡한 자기 조립체 형상의 규명 연구 등에 널리 활용될 수 있으리라 기대한다.

This study presents fabrication of bi-compartmental particles labeled by multiple fluorescence. To compartmentalize fluorescent expression at the particle, two fluorescent dyes with less overlap of the excitation and emission spectra are selected. To ensure the fluorescence stability, the fluorescent dyes contain acrylate functional groups in the molecules so that they can be cross-linked together with monomers constituting the particle. Strong fluorescent expression and compartmentalization were observed at the particle fabricated using the selected fluorescent dyes through confocal microscopy. Furthermore, long-term fluorescence stability was verified by measuring fluorescent expression and intensity for 4 weeks. We anticipate that the bi-compartmental particles labeled by multiple fluorescence can be widely used for multi-target drug delivery system, analysis of 3 dimensional Brownian motion, and investigation of 3 dimensional complex self-assembled morphologies.

키워드

HHGHHL_2018_v56n6_826_f0001.png 이미지

Fig. 1. Schematics for fabricating of fluorescent labeled bi-compartmental particles via the micromolding method.

HHGHHL_2018_v56n6_826_f0002.png 이미지

Fig. 3. (a) The confocal microscope image of the hydrophilic part labeled by nile blue acrylamide (NBAM) at 638 nm laser. (b) The confocal microscope image of the hydrophobic part labeled by fluorescein o-acrylate (FA) at 488 nm laser. (c) The merged images of (a) and (b). (d) Measurement of fluorescence intensity of bi-compartmental particles along the white dotted line of the inserted image. Scale bars are 50 μm.

HHGHHL_2018_v56n6_826_f0003.png 이미지

Fig. 5. Long-term fluorescence stability of bi-compartmental particles containing fluorescein o-acrylate (FA) and nile blue acrylamide (NBAM).

HHGHHL_2018_v56n6_826_f0004.png 이미지

Fig. 2. (a) Chemical structures of fluorescent dyes. (b) Excitation and emission spectra of fluorescein o-acrylate (FA), nile blue acrylamide (NBAM).

HHGHHL_2018_v56n6_826_f0005.png 이미지

Fig. 4.Water contact angle measurements of the hydrophilic film without nile blue acrylamide (NBAM), the hydrophilic film with nile blue acrylamide (NBAM), the hydrophobic film without fluorescein o-acrylate (FA), and the hydrophobic film with fluorescein o-acrylate (FA).

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