한국군사과학기술학회지 (Journal of the Korea Institute of Military Science and Technology)

  • 제20권5호
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  • Pages.726-732
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  • 2017
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  • 1598-9127(pISSN)
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  • 2636-0640(eISSN)
한국군사과학기술학회 (The Korea Institute of Military Science and Technology)
권호 표지
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실시간 중합효소 연쇄반응을 활용한 생물작용제 검증시스템 연구

A Study on the Validation system of Detection for Biological Agents Using Real-Time PCR

  • 차영길 ((주)바이오니아 진단과학시약연구소) ;
  • 구본우 ((주)바이오니아 진단과학시약연구소) ;
  • 김성주 (국방과학연구소 제5기술연구본부) ;
  • 김남일 ((주)바이오니아 진단과학시약연구소) ;
  • 박한오 ((주)바이오니아 진단과학시약연구소)
  • Cha, Younggil (Molecular Diagnostic Division, Bioneer Corp.) ;
  • Koo, Bonwoo (Molecular Diagnostic Division, Bioneer Corp.) ;
  • Kim, Seongjoo (The 5th Research and Development Institute, Agency for Defense Development) ;
  • Kim, Namil (Molecular Diagnostic Division, Bioneer Corp.) ;
  • Park, Hanoh (Molecular Diagnostic Division, Bioneer Corp.)
  • 투고 : 2016.11.11
  • 심사 : 2017.09.22
  • 발행 : 2017.10.05
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초록

Bacillus anthracis, Vibrio cholerae, Variola virus and Shigella dysenteriae are classified as category A and B biological weapons. In this study suggest that 4 genes of Bacillus anthracis, 2 genes of Vibrio cholerae, 1 gene of Variola virus and 1 gene of Shigella dysenteriae were detective 50~500 fg of target DNA per reaction using real-time PCR based assay. Also analytical specificity did not show any cross-reactivity with other related bacteria. Reliable and one reaction could be effective early diagnostic and treatment for detection of unknown samples.

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참고문헌

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