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Sclerotinia Rot on Basil Caused by Sclerotinia sclerotiorum in Korea

Sclerotinia sclerotiorum에 의한 바질 균핵병

  • Hahm, Soo Sang (Agricultural Environment Research Division, Research and Development Bureau, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Kim, Byoung Ryun (Agricultural Environment Research Division, Research and Development Bureau, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Han, Kwang Seop (Agricultural Environment Research Division, Research and Development Bureau, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Kwon, Mi Kyung (Agricultural Environment Research Division, Research and Development Bureau, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Park, In Hee (Agricultural Environment Research Division, Research and Development Bureau, Chungcheongnam-do Agricultural Research & Extension Services)
  • 함수상 (충청남도농업기술원 기술개발국 농업환경과) ;
  • 김병련 (충청남도농업기술원 기술개발국 농업환경과) ;
  • 한광섭 (충청남도농업기술원 기술개발국 농업환경과) ;
  • 권미경 (충청남도농업기술원 기술개발국 농업환경과) ;
  • 박인희 (충청남도농업기술원 기술개발국 농업환경과)
  • Received : 2016.10.29
  • Accepted : 2017.01.06
  • Published : 2017.03.31

Abstract

During growing season of 2011 to 2013, Sclerotinia rot symptoms consistently have been observed on basil in Yesan-gun, Chungcheongnam-do in Korea. The typical symptom formed initially brownish spot on leaf and stem, and then advancing margins, wilting the whole plant and blighting, eventually died. On the surface of diseased lesions was observed cottony, white, dense mat of mycelial growth, and sclerotia ($30-100{\mu}m$ diameter) formed on stem and leaf. Morphological and cultural characteristic on potato dextrose agar, color of colony was white and colorless chocolate, sclerotium of irregular shape of the oval was black and $5-50{\mu}m$ diameter in size. In pathogenicity test, necrosis and wilt of the inoculated stem were observed in all plants and the pathogen was reisolated from stems. On the basis of mycological characteristics, pathogenicity, and internal transcribed spacer rDNA sequence analysis, this fungus was identified as Sclerotinia sclerotiorum. This is the first report of Sclerotinia rot on basil caused by S. sclerotiorum in Korea.

2011년부터 2013년까지 예산 지역의 농가포장에서 재배 중인 바질에서 균핵병으로 의심되는 증상이 지속적으로 관찰되었다. 초기에는 잎과 줄기가 갈색으로 변하기 시작하여 반점이 커지면서 점차 위쪽으로 진전, 위조하여 결국 전체적으로 썩어 죽는다. 잎과 줄기의 병반부에는 다량의 흰색 균사가 발생하며 $30-100{\mu}m$ 직경의 균핵이 형성하였다. PDA상에 균체는 흰색과 옅은 초콜릿 흑색으로 다양한 색을 나타내었고, 균핵은 검은색으로 대부분 불규칙한 구형이며 크기는 $5-50{\mu}m$였다. 병원균을 인위적으로 접종한 바질의 잎과 줄기에서는 자연 발생된 바질과 동일한 괴사와 위조증상을 보이며, 병반에서 역시 같은 균을 분리하였다. 이상과 같이 병원균의 균학적 특징, ITS 염기서열 분석, 병원성 검정 결과 본 병해는 Sclerotinia sclerotiorum에 의한 바질균핵병으로 명명할 것을 제안한다.

Keywords

References

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