생약학회지 (Korean Journal of Pharmacognosy)

  • 제48권3호
  • /
  • Pages.243-247
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  • 2017
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

민박쥐나물 추출물의 Caffeoylquinic Acid 정량 및 Acetylcholinesterase 활성과 Peroxynitrite 농도에 대한 효과

Quantification of Caffeoylquinic Acids and the Effect of the Cacalia hastata var. orientalis Extract on Acetylcholinesterase Activity and Peroxynitrite Concentration

  • Nugroho, Agung (Department of Agro-Industrial Technology, Lambung Mangkurat University) ;
  • Choi, Jae Sue (Department of Food Nutrition, Pukyong University) ;
  • Park, Hee-Juhn (Department of Pharmaceutical Engineering, Sangji University)
  • 투고 : 2017.08.14
  • 심사 : 2017.09.18
  • 발행 : 2017.09.30
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초록

To find the anti-Alzheimer's activity of Cacalia hastata var. orientalis (Compositae), caffeoylquinic acids were analyzed in this plant by HPLC and the inhibitory activities of the extract on cholinesterases including acetylcholinesterase (AChE) and butyrylcholinesterse (BChE) and peroxynitrite ($ONOO^-$) were assayed. Sum of caffeoylquinic acids in the 80% MeOH extract was quantitatively higher in leaves (35.8% of the extract) than in stems (3.7%). The compound, 3,5-dicaffeoylquinic acid, was contained in the highest amount in the leaf (44.32 mg/g dry weight). The $IC_{50}s$ of the 80% MeOH extract were shown to be $67.45{\mu}g/ml$ for AChE, and $8.59{\mu}g/ml$ (for $ONOO^-$), respectively, suggesting that the leaves would have the anti-Alzheimer's activity due to the high content of caffeoylquinic acids.

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참고문헌

  1. Fang, Z., Jeong, S. Y., Jung, H. A., Choi, J. S., Min, B. S. and Woo, M. H. (2010) Anticholinesterase and antioxidant constituents from Gloiopeltis furcate. Chem. Pharm. Bull. 58: 1236-1239. https://doi.org/10.1248/cpb.58.1236
  2. Han, J., Miyamae, Y., Shigemori, H. and Isoda, H. (2010) Neuroprotective effect of 3,5-di-O-caffeoylquinic acid on SH-SY5Y cells and senescence-accelerated-prone mice 8 through the up-regulation of phosphoglycerate kinase-1. Neurosci. 169: 1039-1045. https://doi.org/10.1016/j.neuroscience.2010.05.049
  3. Georgiev, M., Alipieva, K., Orhan, I., Abrashev, R., Denev, P. and Angelova, M. (2011) Antioxidant and cholinesterases inhibitory activities of Verbascum xanthophoeniceum Griseb. and its phenylethanoid glycosides. Food Chem. 128: 100-105. https://doi.org/10.1016/j.foodchem.2011.02.083
  4. Moniruzzaman, M., Asaduzzaman, M., Hossain, M. S., Sarker, J., Rahman, S. M. A., Rashid, M. and Rahman, M. M. (2015) In vitro antioxidant and cholinesterase inhibitory activities of methanolic fruit extract of Phyllanthus acidus. BMC Complement. Altern. Med. 15: 403-412. https://doi.org/10.1186/s12906-015-0930-y
  5. Luo, W., Chen, Y., Wang, T., Hong, C., Chang, L. P., Chang, C. C., Yang, Y. C., Xie, S. Q. and Wang, C.J. (2016) Design, synthesis and evaluation of novel 7-aminoalkyl-substituted flavonoid derivatives with improved cholinesterase inhibitory activities. Bioorg. Med. Chem. 24: 672-680. https://doi.org/10.1016/j.bmc.2015.12.031
  6. Nugroho, A., Song, B. M., Seong, S. H., Choi, J. S., Choi, J. W., Choi, J. Y. and Park, H. J. (2016) HPLC analysis of phenolic substances and anti-Alzheimer's activity of Korean Quercus Species. Nat. Prod. Sci. 22: 299-306. https://doi.org/10.20307/nps.2016.22.4.299
  7. Patcher, P., Obrosova, I. G., Mabley, J. G. and Szabo, C. (2005) Role of nitrosative stress and peroxynitrite in the pathogenesis of diabetic complications. Emerging new therapeutical strategies. Curr. Med. Chem. 12: 267-275. https://doi.org/10.2174/0929867053363207
  8. Choi, R. J., Roy, A., Jung, H. J., Ali, M. Y., Min, B. S., Park, C. H., Yokozawa, T., Fan, T. P., Choi, J. S. and Jung, H. A. (2016) BACE1 molecular docking and anti-Alzheimer's disease activities of ginsenosides. J. Ethnopharmacol. 190: 219-230. https://doi.org/10.1016/j.jep.2016.06.013
  9. Park, H. J. (2010) Chemistry and pharmacological action of caffeoylquinic acid derivatives and pharmaceutical utilization of chwinamul (Korean mountainous vegetable). Arch. Pharm. Res. 33: 1703-1720. https://doi.org/10.1007/s12272-010-1101-9
  10. Nugroho, A., Choi, J. S. and Park, H. J. (2016) Analysis of flavonoid composition of Korean herbs in the family of Compositae and their utilization for health. Nat. Prod. Sci. 22: 1-12. https://doi.org/10.20307/nps.2016.22.1.1
  11. Kimura, K., Sakamoto, Y., Fujisawa, N., Uesugi, S., Aburai, N., Kawada, M., Ohba, S., Yamori, T., Tsuchiya, E. and Koshino, H. (2012) Cleavage mechanism and anti-tumor activity of 3,6-epidioxy-1,10-bisaboladiene isolated from edible wild plants. Bioorg. Med. Chem. 20: 3887-3897. https://doi.org/10.1016/j.bmc.2012.04.025
  12. Miyazawa, M., Kawauchi, Y., Utsumi, Y. and Takahashi, T. (2010) Character impact odorants of wild edible plant-Cacalia hastata L. var. orientalis-Used in Japanese traditional food. J. Oleo Sci. 59: 527-533. https://doi.org/10.5650/jos.59.527
  13. Ellman, G. L., Courtney, D. and Andres, K. D. V. (1961) Featherstone R.M., A new and rapid colorimetric activity. Biochem. Pharmacol. 7: 88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  14. Kooy, N. W., Royall, J. A., Ischiropoulos, H. and Beckman, J. S. (1994) Free Radic. Biol. Med. 16: 149-156. https://doi.org/10.1016/0891-5849(94)90138-4
  15. Kwon, S. H., Lee, H. K., Kim, J. A., Hong, S. I., Kim, H. C., Jo, T. H., Park, Y. I., Lee, C. K., Kim, S. Y. and Jang, C. G. (2010) Neuroprotective effects of chlorogenic acid on scopolamine-induced amnesia via anti-acetylcholinesterase and anti-oxidative activities in mice. Eur. J. Pharmacol. 649: 210-217. https://doi.org/10.1016/j.ejphar.2010.09.001