Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Effect of Sorghum nervosum extract on an anti-inflammatory and cytoprotective

고량(Sorghum nervosum)추출물의 항염증 및 세포 보호 효과

  • Lee, Ju-Hyun (Department of Bioengineering, Konkuk University) ;
  • Kim, Kum-Lan (Department of Doowon Technical University, Gyeonggi Industrial Technical Education Center) ;
  • Moon, Ji-sun (Department of Beauty Health, Jungwon University)
  • 이주현 (건국대학교 생물공학과) ;
  • 김금란 (두원공과대학교 경기 산업기술 교육센터) ;
  • 문지선 (중원대학교 뷰티헬스학과)
  • Received : 2017.08.02
  • Accepted : 2017.09.03
  • Published : 2017.09.30
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Abstract

This study was intended to test anti-inflammation and cytoprotective effect against UVB after Sorghum nervosum was extracted with 70% ethanol. The efficacy of Sorghum nervosum was assessed regarding cell viability analysis, reactive oxygen species measurement, anti-inflammation, a change in COX-2 protein, and cytoprotective effect against UVB. According to the results of experiment, the cell viability of 97% or higher was shown at all concentrations of Sorghum nervosum in RAW264.7 macrophage, HaCaT cell. And in anti-inflammatory NO inhibitory activity, a concentration-dependent inhibitory effect was shown. And COX-2 protein expression was also significantly (p<.001) inhibited at 25, $50{\mu}g/mL$. With regard to cytoprotective effect against UVB, in the quantitative analysis results of reactive oxygen species within the cell, it was verified that Sorghum nervosum extract had an effect on an decrease in the total amount of ROS. When the results of study are considered comprehensively, it is thought that there is possibility of Sorghum nervosum development as raw materials for cosmetics showing an anti-inflammation and cytoprotective function against UVB.

본 연구에서는 고량(Sorghum nervosum) 70 % 에탄올 추출 후 항염증 및 UVB에 대한 세포 보호 효과를 검증하고자 하였다. 고량 추출물의 효능 평가는 세포생존율분석, 활성산소 측정, 항염증, COX-2 단백질 변화, UVB에 대한 세포 보호 효과를 수행하였다. 실험 결과, RAW264.7 대식세포, HaCaT 세포에서 고량추출물 모든 농도에서 97 %이상 세포 생존율을 확인하였다. 항염증 NO 생성 억제능에서는 농도 의존적으로 저해 효과가 나타났으며, COX-2 단백질 발현량 역시 25, $50{\mu}g/mL$ 농도에서 유의하게(p<.001) 저해되었다. UVB에 대한 세포 보호 효과로는 세포 내 활성산소종(ROS) 정량 분석 결과, 고량 추출물이 ROS 총 양 감소에 효과가 있음을 확인하였다. 연구결과를 종합적으로 고려해 볼 때, 고량추출물은 항염증 및 UVB에 대한 세포 보호 기능을 나타내는 화장품 원료로서의 개발 가능성이 있다고 사료된다.

Keywords

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