Molecules and Cells

  • 제40권8호
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  • Pages.533-541
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  • 2017
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research

  • 투고 : 2017.07.23
  • 심사 : 2017.08.11
  • 발행 : 2017.08.31
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⟨ 이전 논문 다음 논문 ⟩

초록

Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescribed DNA-binding mode. Due to their modular architecture and flexibility, TALEs have been rapidly developed into artificial gene targeting reagents. Here, we describe the methods used to design these DNA-binding proteins and their key applications in biomedical research.

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참고문헌

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