Korean Journal of Dental Materials (대한치과재료학회지)

Korean Society For Dental Materials (대한치과재료학회)
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Comparison of TheraCal LC, Mineral trioxide aggregate, and Formocresolas pulpotomy agents in rat molar

백서에서 치수절단술에 사용하는 TheraCal LC, MTA 그리고 Formocresol의 비교

  • Lee, Bin-Na (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University,) ;
  • Song, Young-Sang (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University,) ;
  • Lee, Go-Woon (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University,) ;
  • Kim, Young-Hoon (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University,) ;
  • Chang, Hoon-Sang (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University,) ;
  • Hwang, Yun-Chan (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University,) ;
  • Oh, Won-Mann (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University,) ;
  • Hwang, In-Nam (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University,)
  • 이빈나 (전남대학교 치의학전문대학원 치과보존학교실) ;
  • 송영상 (전남대학교 치의학전문대학원 치과보존학교실) ;
  • 이고운 (전남대학교 치의학전문대학원 치과보존학교실) ;
  • 김영훈 (전남대학교 치의학전문대학원 치과보존학교실) ;
  • 장훈상 (전남대학교 치의학전문대학원 치과보존학교실) ;
  • 황윤찬 (전남대학교 치의학전문대학원 치과보존학교실) ;
  • 오원만 (전남대학교 치의학전문대학원 치과보존학교실) ;
  • 황인남 (전남대학교 치의학전문대학원 치과보존학교실)
  • Received : 2017.05.30
  • Accepted : 2017.06.27
  • Published : 2017.06.30
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Abstract

TheraCal LC, a new light-cured, resin-modified calcium silicate-filled base/liner material, has been introduced as a pulpotomy agent. The aim of this study was to evaluate the capacity of hard tissue formation and pulpal response after pulpotomy with TheraCal LC. Twenty-two 9-week-old male rats were anesthetized, cavities were prepared in maxillary first molars and pulps were capped with formocresol (FC), mineral trioxide aggregate (MTA), and TheraCal LC. Specimens obtained from rats were scanned using a high-resolution micro CT system. The specimens were prepared and evaluated histologically, and immunofluorescence assay was performed to assess the dentin matrix protein-1 (DMP-1) expression. On micro CT analysis, the MTA and TheraCal LC groups showed thicker hard tissue formation than the FC group. On hematoxylin and eosin (H&E) staining, MTA and TheraCal LC groups showed dentine bridge formation with vital pulp beneath the materials. On immunofluorescence analysis, DMP-1 was highly expressed in the TheraCal LC group compared to the FC group. TheraCal LC showed similar capacity to form hard tissue as MTA when it was used as a pulpotomy agent. Because of its good manipulation and faster setting time compared to MTA, TheraCal LC could be considered as a good alternative to MTA.

Formocresol (FC)은 치수절단술에 일반적으로 사용되어 온 재료이지만, 재료의 독성 때문에 현재 calcium hydroxide나 mineral trioxide aggregate (MTA)가 치수절단술에 널리 사용되고 있다. 최근 레진계열 calcium silicate 제재인 Theracal LC가 치수 이장재로 개발이 되었으며, 이는 광중합을 통해 경화되기 때문에 사용이 편리해서 MTA를 적용할 수 없는 치아에 사용할 수 있다. 이번 연구의 목적은 FC, MTA 및 Theracal LC를 각각 치수절단술 후에 적용했을 경우 경조직 형성 능력과 치수반응을 비교하는 것이다. Sprague Dawley Rat의 상악 대구치 치수절단술 후 FC, MTA 및 Theracal LC를 적용하였다. 경조직 형성 여부를 확인하기 위해 Skyscan을 사용해 마이크로 컴퓨터 단층촬영(micro CT) 이미지를 획득하고, he matoxylin and e osin (H&E) 염색을 하여 조직학적 반응을 확인하였다. Dentin matrix protein-1 (DMP-1)의 발현을 확인하기 위해 면역형광 염색을 시행하였다. FC를 사용한 시편에서는 경조직 형성이 관찰되지 않았으며, 치수절단술이 시행된 인접면에 염증반응이 관찰되었고 DMP-1발현은 확인되지 않았다. MTA와 Theracal LC를 사용한 시편에서는 경조직 형성이 관찰되었고 DMP-1의 발현이 확인되었다. 결론적으로, MTA나 Theracal LC를 사용한다면 남아있는 치수의 생활력과 기능을 유지시켜 보다 좋은 치료 예후를 기대할 수 있을 것으로 사료된다.

Keywords

Acknowledgement

Supported by : Chonnam National University Hospital Biomedical, National Research Foundation of Korea (NRF)