Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Zinc and Aluminum Hot-dip Galvanized Sheet Steel on the Gill and Hepatopancreas of the Abalone Haliotis discus hannai

아연 및 알루미늄 용융도금 처리된 강판이 북방전복(Haliotis discus hannai)의 아가미와 간췌장에 미치는 영향

  • 이치훈 (제주대학교 해양과학연구소) ;
  • 박준영 (제주대학교 해양과학연구소) ;
  • 이영돈 (제주대학교 해양과학연구소)
  • Received : 2016.05.30
  • Accepted : 2017.08.04
  • Published : 2017.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated the toxicity of zinc and aluminum hot-dip galvanized sheet steel to abalone Haliotis discus hannai via changes in the gill and hepatopancreas using histological and transmission electron microscopy analysis. Experimental groups were composed of one control and four exposure conditions (direct or indirect exposure to zinc and aluminum hot-dip galvanized sheet steel). In the control group, aluminum exposure groups (direct and indirect), and indirect zinc exposure group, abalone mortality was not observed until the end of the experiment, and no histopathological changes were observed in the gill and hepatopancreas. However, the direct zinc exposure group exhibited 100% mortality. Ultrastructural analysis of the cytoplasm of ciliated and microvilli-bearing epithelial cells from gill filaments revealed electron-dense vesicles near the cell membrane and disruption of the nuclear membrane. We also observed swollen mitochondria and a loss of mitochondrial cristae. The hepatopancreas showed similar changes, and we detected highly electron-dense particles within the vesicles. These results suggest that abalone exposed directly to zinc hot-dip galvanized sheet steel experience acute toxicity, causing damage to cell organelles in the gill and hepatopancreas and, finally, inducing mortality.

Keywords

References

  1. Berg JM. 1990. Zinc fingers and other metal-binding domains. Elements for interactions between macromolecules. J Biol Chem 265, 6513-6516.
  2. Bossy-Wetzel E, Talantova MV, Lee WD, Scholzke MN, Harrop A, Mathews E, Gotz T, Han J, Ellisman MH, Perkins GA and Lipton SA. 2014. Crosstalk between nitric oxide and zinc pathways to neuronal cell death involving mitochon-drial dysfunction and p38-activated K+ channels. Neuron 41, 351-365.
  3. Bryan GW, Langston WJ, Hummerstone LG and Burt GR. 1985. A guide to the assessment of heavy- metal contamination in estuaries using biological indicators. In: Marine Biological Association of the United Kingdom, Occasional Publication, U.K., 1-92.
  4. Chang EY, Coon SI, Walch M and Weiner R. 1996. Effects of hyphomonas PM-1 biofilims on the toxicity of copper and zinc to Crassostrea gigas and Crassostrea virginica larval set. J Shellfish Res 15, 589-595.
  5. Ciacci C, Canonico B, Bilanicova D, Fabbri R, Cortese K, Gallo G, Marcomini A, Pojana G and Canesi L. 2012. Immunomodulation by different types of N-oxidesin the hemocytes of the marine bivalve Mytilus galloprovincialis. PLoS ONE 7, e36937. https://doi.org/10.1371/journal.pone.0036937
  6. Dautremepuits C, Paris-Palacios S, Betoulle S and Vernet G. 2004. Modulation in hepatic and head kidney parameters of carp (Cyprinus carpio L.) induced by copper and chitosan. Comp Biochem Physiol 137, 325-333.
  7. Guo D, Bi H, Liu B, Wu Q, Wang D and Cui Y. 2013. Reactive oxygen species-induced cytotoxic effects of zinc oxide nanoparticles in rat retinal ganglion cells. Toxicol In Vitro 27, 731-738. https://doi.org/10.1016/j.tiv.2012.12.001
  8. Ho E. 2004. Zinc deficiency, DNA damage and cancer risk. J Nutr Biochem 15, 572-578. https://doi.org/10.1016/j.jnutbio.2004.07.005
  9. Hunt JW and Anderson BS. 1989. Sublethal effects of zinc and municipal effluents of larvae of the red abalone, Haliotis rufescens. Mar Biol 101, 545-552. https://doi.org/10.1007/BF00541657
  10. Hwang UK, Heo S, Park JS and Kang HS. 2012. Effects of Lead and Zinc on the Fertilization and Embryo Development of the Sea Urchin (Hemicentrotus pulcherrimus). Korean J Environ Biol 30, 128-135.
  11. Ibrahim A, Mackinnon BM and Burt MDB. 2000. The influence of sub-lethal levels on zinc on osmoltifying Atlantic salmon Salmo salar and on their subsequent susceptibility to infection with Lepeophtheirus salmonis. Contrib Zool, 69. Retrieved from http://www.ctoz.nl/vol69/nr01/a13 on Oct 17, 2016.
  12. Kamaruzzaman BY, Akbar B, Jala KCA and Shahbudin S. 2010. Accumulation of metals in the gills of tilapia fingerlings (Oreochromis niloticus) from in vitro toxicology study. J Fish Aquat Sci 5, 503-509. http://10.3923/jfas.2010.503.509.
  13. Ju SM, Lee JW, Jin YG, Yu J and Lee JS. 2006. Effect of zinc bioaccumulation on survival rate, activity, growth and organ structure of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). J Environ Toxicol 21, 115-126.
  14. Liao CM and Lin MC. 2001. Toxicokinetics and acute toxicity of waterborne zinc in abalone (Haliotis diversicolor supertexta Lischke). Bull Environ Contam Toxicol 66, 597-602.
  15. Liao CM and Chou YH. 2005. Site-specific environmental quality criteria for survival and growth of farmed abalone exposed to zinc. Aquaculture 249, 159-173. https://doi.org/10.1016/j.aquaculture.2005.02.044
  16. Maity S, Roy S, Chaudhury S and Bhattacharya S. 2008. Antioxidant responses of the earthworm Lampito mauritii exposed to Pb and Zn contaminated soil. Environ Pollut 151, 1-7. https://doi.org/10.1016/j.envpol.2007.03.005
  17. McGeer JC, Szebedinszky C, McDonald DG and Wood CM. 2000. Effects of chronic sublethal exposure to waterborne Cu, Cd or Zn in rainbow trout. 1: Iono-regulatory disturbance and metabolic costs. Aquat Toxicol 50, 231-243. https://doi.org/10.1016/S0166-445X(99)00105-8
  18. Santos DCM, Matta SLP, Oliveria JA and Santos JAD. 2011. Histological alterations in gills of Astyanax aff. Bimaculatus caused by acute exposition to zinc. Exp Toxicol Pathol 64, 861-866.
  19. Seo DC, Choi SD, Rha SJ, Yang HC and Suh HL. 1999. Effects of zinc toxicity on larvae development and seed collection of the abalone, Haliotis discus hannai. J Aquaculture 12, 229-236.
  20. Son MH, Kim BH, Kim S and Lee JS. 2015. Histological indicator change of the abalone, Haliotis discus hannai chronic exposed to Zinc Chloride ($ZnCl_2$). Korean J Malacol 31, 195-201. https://doi.org/10.9710/kjm.2015.31.3.195
  21. Trevisan R, Delapedra G, Mello DF, Arl M, Schmidt EC, Meder F, Monopoli M, Cargnin-Ferreira E, Bouzon ZL, Fisher AS, Sheehan D and Dafre AL. 2014. Gills are an initial target of zinc oxide nanoparticles in oysters Crassostrea gigas, leading to mitochondrial disruption and oxidative stress. Aquat Toxicol 153, 27-38. https://doi.org/10.1016/j.aquatox.2014.03.018
  22. Tsai JW, Chou YH, Chen BC, Liang HM and Liao CM. 2004. Growth toxicity bioassays of abalone Haliots diversicolor supertexta exposed to waterborne zinc. Bull Environ Contam Toxicol 72, 70-77. https://doi.org/10.1007/s00128-003-0242-7
  23. Viarengo A. 1985. Biochemical effects of trace metals. Mar Pollut Bull 16, 153-158. https://doi.org/10.1016/0025-326X(85)90006-2
  24. Yu KN, Yoon TJ, Minai-Tehrani A, Kim JE, Park Sh, Jeong MS, Ha SW, Lee JK, Kim JS and Cho MH. 2013. Zinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generation. Toxicol In Vitro 27, 1187-1195. https://doi.org/10.1016/j.tiv.2013.02.010