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http://dx.doi.org/10.5657/KFAS.2017.0388

Effects of Zinc and Aluminum Hot-dip Galvanized Sheet Steel on the Gill and Hepatopancreas of the Abalone Haliotis discus hannai  

Lee, Chi Hoon (Marine Science Institute, Jeju National University)
Park, Jun Young (Marine Science Institute, Jeju National University)
Lee, Young Don (Marine Science Institute, Jeju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.50, no.4, 2017 , pp. 388-395 More about this Journal
Abstract
We investigated the toxicity of zinc and aluminum hot-dip galvanized sheet steel to abalone Haliotis discus hannai via changes in the gill and hepatopancreas using histological and transmission electron microscopy analysis. Experimental groups were composed of one control and four exposure conditions (direct or indirect exposure to zinc and aluminum hot-dip galvanized sheet steel). In the control group, aluminum exposure groups (direct and indirect), and indirect zinc exposure group, abalone mortality was not observed until the end of the experiment, and no histopathological changes were observed in the gill and hepatopancreas. However, the direct zinc exposure group exhibited 100% mortality. Ultrastructural analysis of the cytoplasm of ciliated and microvilli-bearing epithelial cells from gill filaments revealed electron-dense vesicles near the cell membrane and disruption of the nuclear membrane. We also observed swollen mitochondria and a loss of mitochondrial cristae. The hepatopancreas showed similar changes, and we detected highly electron-dense particles within the vesicles. These results suggest that abalone exposed directly to zinc hot-dip galvanized sheet steel experience acute toxicity, causing damage to cell organelles in the gill and hepatopancreas and, finally, inducing mortality.
Keywords
Zinc; Aluminum; Hot-dip galvanized steel sheet; Toxicity; Abalone Haliotis discus hannai;
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