Journal of Korean society of Dental Hygiene (한국치위생학회지)

Korean Society of Dental Hygiene (한국치위생학회)
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Antimicrobial activities of Ramaria botrytis (Fr.) against oral bacteria

싸리버섯 추출물의 구강세균에 대한 항균활성

  • Kim, Ki-Hwa (Department of Life Science and Biochemical Engineering, Graduate School, Sunmoon University) ;
  • Han, So-Ra (Department of Life Science and Biochemical Engineering, Graduate School, Sunmoon University) ;
  • Kim, Byeol-Lee (Department of BT-Convergent Pharmaceutical Engineering, Sunmoon University) ;
  • Jung, Sang-Hee (Department of Dental Hygiene, GangneungYeongdong University) ;
  • Oh, Tae-Jin (Department of BT-Convergent Pharmaceutical Engineering, Sunmoon University)
  • 김기화 (선문대학교 일반대학원 생명공학과) ;
  • 한소라 (선문대학교 일반대학원 생명공학과) ;
  • 김별이 (선문대학교 건강보건대학 BT융합제약공학과) ;
  • 정상희 (강릉영동대학교 치위생과) ;
  • 오태진 (선문대학교 건강보건대학 BT융합제약공학과)
  • Received : 2017.04.27
  • Accepted : 2017.05.30
  • Published : 2017.06.30
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Abstract

Objectives: This study aimed to find out the antimicrobial activities of Ramaria botrytis (Fr.) extracts against oral pathogens. Methods: The antimicrobial activities of Ramaria botrytis (Fr.) extracts were evaluated against oral pathogens by the disc diffusion assay, and the minimum inhibitory concentrations (MICs) of ethyl acetate extracts were determined by broth dilution method. The strains used in this study were Staphylococcus aureus, Streptococcus mutans, Streptococcus sanguinis, Streptococcus sobrinus, Streptococcus anginosus, Streptococcus ratti, Streptococcus criceti, Actinomyces israelii, Actinomyces viscosus and Aggregatibacter actinomycetemcomitans. Results: The ethyl acetate extract of Ramaria botrytis (Fr.) effectively inhibited the growth of oral bacteria compared with acetone or ethanol extract. The ethyl acetate extract exhibited MIC values ranging from 3.75 to 15.00 mg/ml, and it showed antimicrobial activity against both Gram-positive and negative oral bacteria. Conclusions: The ethyl acetate extracts from Ramaria botrytis (Fr.) showed the antimicrobial activities against ten oral bacteria. Thus, the extract of Ramaria botrytis (Fr.) may be considered as an effective natural antimicrobial agent for the prevention of oral pathogens.

Keywords

References

  1. Kim HJ, Lee IS. Antimutagenic and cytotoxic effects of korean wild mushrooms extracts. Korean J Food Sci Technol 2004;36:662-8.
  2. Lee SL, Kim JG. Anti-microbial activity of soybean extract against oral microbes. Korean J Environ Health Sci 2006;32:192-7.
  3. Kozai K, Miyake Y, Kohds H, Kametaka S, Yamasaki K, Suginaka H, et al. Inhibition of glucosyltransferase from Streptococcus mutans by oleanolic acid and ursolic acid. Caries Res 1987;21:104-8. https://doi.org/10.1159/000261010
  4. Gibbons RJ, Hay DI. Adsorbed salivary acidic proline-rich proteins contribute to the adhesion of Streptococcus mutans JBP to apatitic surfaces. J Dent Res 1989;68:1303-7. https://doi.org/10.1177/00220345890680090201
  5. Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontol 2000 1994;5:78-111. https://doi.org/10.1111/j.1600-0757.1994.tb00020.x
  6. Darveau RP, Tanner A, Page RC. The microbial challenge in periodontitis. Periodontol 2000 1997;14:12-32. https://doi.org/10.1111/j.1600-0757.1997.tb00190.x
  7. Jarvine H, Tenovuo J, Huovinen P. In vitro susceptibility of Streptococcus mutans to chlorhexidine, and six other antimicrobial agents. Antimicrob Agent Chemother 1993;37:1158-9. https://doi.org/10.1128/AAC.37.5.1158
  8. Ullsfoss BN, Ogaard B, Arends J, Ruben J, Rolla G, Afseth J. Effect of a combined chlorhexidine and NaF mouthrinse: an in vivo human caries model study. Scand J Dent Res 1994;102:109-12.
  9. Loe H. Schiott CR, Karring G, Karring T. Two years oral use of chlorhexidine in man. J Periodontal Res 1976;11:135-44. https://doi.org/10.1111/j.1600-0765.1976.tb00061.x
  10. Baek DH. Screening of the natural plant extracts for the antimicrobial activity on dental pathogens. Korean J Microbiol 2007;43:227-31.
  11. Kim HS, Ha HC, Kim TS. Research and prospects in new functional mushrooms - Tremella fuciformis, Grifora frondosa, and Hypsizigus marmoreus. Food Sci Ind 2003;36:42-6.
  12. Choi SJ, Lee YS, Kim JK, Kim JK, Lim SS. Physiological activities of extract from edible mushrooms. J Korean Soc Food Sci Nutr 2010;39:1087-96. https://doi.org/10.3746/jkfn.2010.39.8.1087
  13. Han SR, Lim KO, Oh TJ. Antimicrobial activities against oral bacteria and growth inhibition against Actinomyces viscosus using Lentinus edodes various extracts. J Korean Soc Dent Hyg 2015;15:735-41. https://doi.org/10.13065/jksdh.2015.15.04.735
  14. Pyo MY, Ro IH. A study on the amino acid contents of edible mushrooms. Korean J Nutr 1975;8:47-59.
  15. Hwang BH, Lee TS. Extractive compounds of Ramaria formosa(Fr.) Quel. J Kor For En 2003;22:37-42.
  16. Kim HJ, Lee IS, Bae JT, Kim OM, Park SH, Chang JS, et al. Effect of Ramaria botrytis methanol extract on hepatotoxicity in benzo(${\alpha}$)pyrene-treated mice and expression of cytochrome P-450 1A1 isozyme. Korean J Mycol 2003;31:34-9. https://doi.org/10.4489/KJM.2003.31.1.034
  17. Seoh JH, Cho SY, Lee SW. Study on the tasty constituents and minerals in Clavariaceae botrytis. J Koeran Soc Food Nutr 1974;3:17-21.
  18. Kim HJ, Lee IS, Lee KR. Antimutagenic and anticancer effects of Ramaria botrytis(Fr.) Rick extracts. J Korean Soc Food Sci Nutr 1999;28:1321-5.
  19. Kim HJ, Lee KR. Effect of Ramaria botrytis methanol extract on antioxidant enzyme activities in benzo(${\alpha}$)pyrene-treated Mice. Korean J Food Sci Technol 2003;35:286-90.
  20. Lee HO, Kim CH, Lim JA, Lee MH, Baek SH. Antimicrobial effect of Puerariae thunbergiana extracts against oral microorganism. J Dent Hyg Sci 2004;4:45-8.
  21. Kim EM, Cho MJ. Antimicrobial activities of oral bacteria by lichen extracts. J Korean Soc Dent Hyg 2012;12:81-91. https://doi.org/10.13065/jksdh.2012.12.1.081
  22. Jang JH, Kim YI, Lee H. Antimicrobial activity of Prunus mume extract to oral microbes. J Korean Soc Dent Hyg 2014;14:109-15. https://doi.org/10.13065/jksdh.2014.14.01.109
  23. Piddock LJ. Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. J Appl Bacteriol 1990;11:151-7.
  24. Park SN, Lee DK, Lim YK, Kim HS, Cho EG, Jin DC, et al. Antimicrobial effect of carvacrol against cariogenic and periodontopathic bacteria. Korean J Microbiol 2012;48:52-6. https://doi.org/10.7845/kjm.2012.48.1.052
  25. Katsuhiko F, Tadash I, Yukihiko H. Antibacterial activity of tea polyphenols against phytopathogenic bacteria. Agric Bio Chem 1991;55:1895-7. https://doi.org/10.1080/00021369.1991.10870886
  26. Park EJ, Lee JS, Choi WS. Anti-cariogenic activities of mushroom extracted with various solvent systems. Korean J Food Sci Technol 2011;43:783-6. https://doi.org/10.9721/KJFST.2011.43.6.783
  27. Lien HM, Tseng CJ, Huang CL, Lin YT, Chen CC, Lai YY. Antimicrobial activity of Antrodia camphorata extracts against oral bacteria. PLos One 2014;9:e105286. https://doi.org/10.1371/journal.pone.0105286