Journal of Dental Rehabilitation and Applied Science (구강회복응용과학지)

Korean Academy of Stomatognathic Function and Occlusion (대한턱관절교합학회)
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Interaction of Hydroxyethylidene bisphosphonate (HEBP) with other endodontic irrigants on tissue dissolving capacity and antimicrobial effect

근관세정제와 상호작용시 Hydroxyethylidene bisphosphonate (HEBP)의 조직용해능력, 항균효과에 대한 연구

  • Kim, Ranah (Department of Conservative Dentistry, Veterans Health Service Medical Center) ;
  • Kim, Yoon Gun (Department of Conservative Dentistry, Veterans Health Service Medical Center) ;
  • Kim, Mi-Yeon (Department of Conservative Dentistry, Veterans Health Service Medical Center) ;
  • Song, Byung Chul (Department of Conservative Dentistry, Veterans Health Service Medical Center) ;
  • Kim, Sun-ho (Department of Conservative Dentistry, Veterans Health Service Medical Center) ;
  • Kim, Jeong-hee (Department of Conservative Dentistry, Veterans Health Service Medical Center)
  • 김난아 (한국보훈복지공단 중앙보훈병원 치과보존과) ;
  • 김윤건 (한국보훈복지공단 중앙보훈병원 치과보존과) ;
  • 김미연 (한국보훈복지공단 중앙보훈병원 치과보존과) ;
  • 송병철 (한국보훈복지공단 중앙보훈병원 치과보존과) ;
  • 김선호 (한국보훈복지공단 중앙보훈병원 치과보존과) ;
  • 김정희 (한국보훈복지공단 중앙보훈병원 치과보존과)
  • Received : 2017.03.16
  • Accepted : 2017.05.16
  • Published : 2017.06.30
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Abstract

Purpose: The purpose of this study was to evaluate tissue dissolving capacity, antimicrobial effect of Hydroxyethylidene bisphosphonate (HEBP) interacting with sodium hypochlorite (NaOCl), Ethylenediaminetetraacetic acid (EDTA) as conventional endodontic irrigants and to determine tissue dissolving efficacy depended on temperature. Materials and Methods: A total of 80 bovine muscles were randomly distributed into 8 groups (n = 10). After their initial weights determined on a precision scale, the specimens in each group were immersed in the solutions for 5, 10 and 15 min and reweighted at each time period. Agar diffusion test inoculated with Enterococcus faecalis was performed for antimicrobial effect of each endodontic irrigants. Results: The ability to dissolve organic matter was greater in NaOCl group following NaOCl and HEBP mixture. Heated NaOCl ($40^{\circ}C$) and NaOCl/ HEBP mixture was greater tissue dissolving efficacy than room temperature ($25^{\circ}C$). Antimicrobial effect was greater and significant in the following order EDTA > EDTA + 1% NaOCl > $1%\;NaOCl{\geq}1%\;NaOCl$ + HEBP. Conclusion: HEBP as soft chelating agent does not disturb antimicrobial effect and less affected tissue dissolving efficacy as inherent properties of NaOCl. In the heated NaOCl/HEBP mixture analyzed, it dissolved more the organic matter than room temperature.

목적: 근관 세정제로서 Hydroxyethylidene bisphosphonate (HEBP)가 다른 근관 세정제인 Sodium hypochlorite (NaOCl), Etylenediaminetetraacetic acid (EDTA) 상호 작용 시 조직용해 능력, 항균효과와 온도에 따른 조직 용해 능력의 변화에 대해 알아보았다. 연구 재료 및 방법: 소의 근육으로 제작된 동일한 단면적($2{\times}2{\times}2mm$)을 가진 80개의 시편을 8개의 그룹(n = 10)으로 임의로 나누었다. 초기 무게 측정 후 각각의 제조된 근관 세정제에 넣어 5분, 10분, 15분 후 무게를 측정하여 무게변화율을 구하였다. 항균 효과 조사를 위해 Enterococcus faecalis를 접종한 agar diffusion test를 시행하였다. 결과: 조직 용해 능력은 NaOCl group (G4, 5)이 가장 뛰어났으며, NaOCl과 HEBP의 혼합물(G7, 8)이 다음으로 나타났다. 또한 가열된($40^{\circ}C$) NaOCl과 NaOCl/HEBP의 혼합물의 경우 실온($25^{\circ}C$)보다 우수한 조직용해 능력이 나타났다. 항균 효과의 경우 EDTA > EDTA + 1% NaOCl > $1%\;NaOCl{\geq}1%\;NaOCl$ + HEBP 순으로 나타났다. 결론: HEBP는 NaOCl 의 항균 효과를 방해하지 않으며, 조직 용해 능력에도 기존의 킬레이팅 제재에 비해 적은 영향을 미친다. 가열된 NaOCl, NaOCl과 HEBP의 혼합재제는 실온과 비교 시에 효율적으로 조직을 용해할 수 있다.

Keywords

References

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