Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Puerariae Radix Ethanol Extract on the Proliferation of Human Dermal Papilla Cells

인체 모유두세포의 증식에 미치는 갈근 에탄올추출물의 효과

  • Park, Seol A (Department of Beauty Design Graduate School, Wonkwang University) ;
  • Ko, Kyoung Sook (Department of Beauty Design Graduate School, Wonkwang University) ;
  • In, Myoung Hee (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo, Won Hong (Department of Anatomy, College of Korean Medicine, Wonkwang University)
  • 박설아 (원광대학교 뷰티디자인과) ;
  • 고경숙 (원광대학교 뷰티디자인과) ;
  • 인명희 (한의학전문대학원 한약자원개발학과) ;
  • 문연자 (한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (한의과대학 해부학교실)
  • Received : 2017.04.07
  • Accepted : 2017.06.22
  • Published : 2017.06.25
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Abstract

In this study, we investigated the effect of Puerariae Radix ethanol extracts (EPR). The effect of the EPR on proliferation of human hair dermal papilla cells(HHDPCs) by MTT assay and observed Expression of mechanisms that regulate cell proliferation extracellular signal-regulated kinase(ERK) and Akt by western blot. The results showed EPR increased the proliferation of HHDPCs and up-regulation phosphorylation of ERK and Akt. ERK and Akt increased by EPR inhibited phosphorylation by PD98059 (ERK inhibitor) and LY294002 (Akt inhibitor), and cell proliferation was also inhibited. These results suggested EPR increases the proliferation of HHDPCs through phosphorylation of ERK and Akt, and therefore is a beneficial effect for the alopecia treatment.

Keywords

References

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