Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of kinds of medium and plant growth regulators for plantlets regeneration by bud culture in Disporum sessile

윤판나물(Disporum sessile) 아배양에 의한 식물체 재분화에 영향하는 배지 종류 및 생장조절물질 효과

  • Lee, Na-Nyum (Forest Biotechnology Division, National Institute of Forest Science) ;
  • Kim, Ji-Ah (Forest Biotechnology Division, National Institute of Forest Science) ;
  • Kim, Tae Dong (Forest Biotechnology Division, National Institute of Forest Science) ;
  • Kim, Yong Wook (Forest Biotechnology Division, National Institute of Forest Science)
  • 이나념 (국립산림과학원 산림생명공학과) ;
  • 김지아 (국립산림과학원 산림생명공학과) ;
  • 김태동 (국립산림과학원 산림생명공학과) ;
  • 김용욱 (국립산림과학원 산림생명공학과)
  • Received : 2016.12.06
  • Accepted : 2017.03.29
  • Published : 2017.03.31
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Abstract

This study was conducted to establish the optimal condition for in vitro propagation of Disporum sessile. In the experiments with different kinds of media (MS, B5 and WPM) and explant types for shoot induction and elongation, the highest number of shoot inductions (2.5/explant) was shown when the axillary bud explants were cultured on MS medium without plant growth regulators (PGRs). The best shoot elongation (7.2 cm) was also obtained when the apical buds were cultured on MS medium. The effect of BA pulse treatments with in vitro shoots was also examined. The highest in shoot induction (2.29/explant) and elongation (7.28 cm) was observed when the shoots from axillary buds were cultured on the media without PGRs. However, it decreased with increasing duration of BA pulse-treatment. The highest rooting rate (100%) and number of root inductions (21.3/explant) were achieved with 1.0 mg/L indole-3-butyric acid (IBA) treatment, whereas no differences were observed by different shoot types. The regenerated in vitro plantlets were hardened and successfully established in soil.

본 연구는 윤판나물의 효과적인 기내 증식을 위한 배양 최적 조건을 구명하기 위해 수행되었다. 신초 유도 및 생장에 영향하는 배지(MS, B5 및 WPM) 및 절편체 종류에 따른 실험에서 줄기 유도는 액아 절편을 식물생장조절물질 무첨가 MS 배지에 배양 시 절편 당 2.5개로 가장 좋았고, 줄기 신장은 정아 절편을 식물생장조절물질 무첨가 MS 배지 배양 시 7.2 cm로 가장 효과적이었다. BA 침지 처리 시 신초 유도 효과는 액아배양 유래 신초를 BA 침지 무처리구에서 신초 유도(2.29개/절편) 및 신초 신장(7.28 cm)이 가장 효과적이었으며, BA 침지 시간이 길어짐에 따라 신초 유도 및 길이 생장은 감소하였다. 발근에 영향하는 IBA 농도 효과는 정아유래 신초를 1.0 mg/L 처리구에서 배양 시 최대 발근율(100%) 및 뿌리 유도수(21.3개/절편)를 보였으며, 정아 혹은 액아 유래 신초지 종류에 따른 발근율의 차이는 크지 않은 것으로 관찰되었다. 발근된 식물체는 상토로 이식하여 형태적인 변이 없이 정상적인 생장을 보였다.

Keywords

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