Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Callus induction and plant regeneration from immature zygotic embryos of various maize genotypes (Zea mays L .)

다양한 계통의 옥수수 미성숙배로부터 캘러스 유도와 식물체 재분화

  • Hong, Joon Ki (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Park, Ki Jin (Maize Experiment Station, Gangwondo Agricultural Research and Extension Services) ;
  • Lee, Gang-Seob (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Dool Yi (Crop Foundation Research division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Ju-Kon (Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science & Technology, Seoul National University) ;
  • Lee, Seung Bum (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Suh, Eun Jung (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Yeon-Hee (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration)
  • 홍준기 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 박기진 (강원도 농업기술원 옥수수연구소) ;
  • 이강섭 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 김둘이 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 김주곤 (서울대학교 그린바이오과학기술연구원 종자생명과학연구소) ;
  • 이승범 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 서은정 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 이연희 (농촌진흥청 국립농업과학원 농업생명자원부)
  • Received : 2017.02.17
  • Accepted : 2017.03.17
  • Published : 2017.03.31
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Abstract

We investigated the callus induction and plant regeneration ability of 16 maize genotypes, including the Korean inbred lines, using 9 to 15 day-old immature zygotic embryos from maize grown in pots and from field cultures. Immature zygotic embryos placed on MS medium supplemented with L-proline 0.7 g/L, MES 0.5 g/L, Dicamba 1.5 mg/L, 2,4-D 0.5 mg/L, $AgNO_3$ 4 mg/L, and sucrose 20 g/L, showed the highest frequency of callus induction. The highest number of shoots regenerated when the embryogenic callus were transferred to MS medium supplemented with 5 mg/L zeatin. The root formation was observed when shoots were grown on MS medium supplemented with 0.2 mg/L indole-3-butyric acid (IBA). Additionally, under the same culture conditions, immature zygotic embryos from maize grown in the field also had a high frequency of plant regeneration. Except one genotype, 15 genotypes showed callus induction and shoot regeneration. Among the 16 genotypes tested, H99, B98, HW3, and B73 yielded the best plant regeneration. H99 showed maximum shoot formation from the primary embryogenic callus. The results suggest that genotypes and growth conditions of the maize plant plays very important roles for enhancing the embryogenesis competence of immature zygotic embryos. The successful regeneration from immature zygotic embryos of maize inbred lines provides a basis for molecular breeding of new cultivars by genetic transformation.

옥수수의 최적 조직 배양 조건을 확립하기 위하여 옥수수 국내 5 계통과 국외 11 계통 총 16 계통을 포트와 포장 재배하여 미성숙 배를 분리하여 배발생 캘러스 유도 및 식물체 재분화율을 조사하였다. MS 배지에 auxin으로 1.5 mg/L Dicamba와 0.5 mg/L 2,4-D 가 첨가된 배지에서 캘러스 형성은 본 실험에 사용된 옥수수 계통 모두에서 높은 빈도로 유도되었으며, 캘러스로부터 식물체 재분화는 5mg/L zeatin이 첨가된 재분화 배지에서 높은 재분화율을 보였다. 또한 포장에서 재배된 옥수수로부터 미성숙 배를 분리하여 사용하였을 때 캘러스 유기 및 식물체 재분화 효율이 높았던 것으로 보아 미성숙 배를 분리하기 위한 옥수수 상태 및 genotype이 중요한 영향을 준다는 것을 알 수 있었다. 본 실험을 통하여 배 발생 캘러스 형성 및 식물체 재분화 효율이 조사된 옥수수 계통들은 생명공학 기술을 활용한 신품종 개발을 위한 형질전환 시스템 개발에 유전자원으로 활용될 수 있는 정보를 제공할 것으로 사료된다.

Keywords

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