The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

A Study on the Skin Protection Effects of Cypress Essential Oil on the DNCB-induced Atopic Dermatitis in NC/Nga Mice

DNCB로 아토피피부염을 유발한 NC/Nga mice에서 사이프러스 에센셜 오일의 효능에 관한 연구

  • Park, Chan-Ik (Department of Aroma-Applied Science, Daegu Haany University)
  • 박찬익 (대구한의대학교 힐링산업학부 향산업전공)
  • Received : 2017.04.19
  • Accepted : 2017.05.20
  • Published : 2017.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The application of DNCB (1-chloro-2,4-dinitrochlorobenzene) can cause cellular immunity allergic reaction such as erythema or edema on NC/Nga mice and combinational function of cells participating in immunity increase inflammatory mediator. In this study, the effects of cypress essential oil on NC/Nga mice have been assessed. Methods : Male SPF NC/Nga mice aged 8 weeks have been used for atopic dermatitis induction skin. 1% DNCB was applied on ears and backs of which hair was removed using clipper on $1^{st}$ day and 0.4% DNCB was applied three times a week for 3 weeks. In this study, cypress essential oil has been treated 1 time a day for 3 weeks after application of DNCB to induce atopic dermatitis on skin for further experiments. Results : This study shows that inhalation or application of cypress essential oil reduced edema in ears and the thickness of epidermis induced by DNCB treatment. And it can be known that treatment of cypress essential oil inhibited mast cell proliferation and reduced IgE level similar to that of the negative control especially when cypress essential oil was inhaled by the mice. Synthetic oil showed the effects lower than those of cypress essential oil. Conclusions : Inhalation or direct application of cypress essential oil on skin reduced IgE level in blood and prohibits the proliferation of mast cell, from which it can be known that cypress essential oil can be effectively used to reduce the symptoms of atopic dermatitis.

Keywords

References

  1. Ahn SG, Lee SH, Lee SE, Hong SP, Kim HJ, Lee HG, Park HN, Jeong SK. Skin Barrier. Yeomungak. 2010 : 229-35.
  2. Sung HC, Lee WJ, Lee SJ, Kim DW. A study on the relationship of allergy-related laboratory findings with the clinical severity of atopic dermatitis. Korean J Dermatol. 2006 ; 44 : 1051-7.
  3. Kim KH. Climate change and atopic dermatitis. J Korean Med Assoc. 2011 ; 54(2) : 169-74 https://doi.org/10.5124/jkma.2011.54.2.169
  4. Spergel JM. From atopic dermatitis to asthma:the atopic march. Ann Allergy Asthma Immunol. 2010 ; 105 : 99-106. https://doi.org/10.1016/j.anai.2009.10.002
  5. Dieter Maurer, Edda Fiebiger, Barbel Reininger, Christof Ebner, Peter Petzelbauer, Guo-Ping Shi, Harold A Chapman, Georg Stingl. Fc Epsilon Receptor I on Dendritic Cells Delivers IgE-Bound Multivalent Antigens into a Cathepsin S-Dependent Pathway of MHC Class II Presentation. The Journal of Immunology. 1998 ; 161(6) : 2731-9.
  6. Lee WJ, Choi YH, Kim BA, Park CI. Development of moisturizer for reducing the iching feel in senior dry skin by reinforcing the skin barrier function physically. The Journal of Skin Barrier Research. 2012 ; 14(2) : 65-71.
  7. Lee WJ, Park CI. Application of rheological theory to access emulsion stability and physical skincare barrier. The Journal of Skin Barrier Research. 2013 ; 15(1) : 65-9.
  8. Na KJ, Kang HY, Oh JW, Choi IG, Yun YW, Jeung EB. The sedative effect of stress by essential oils from softwoods. Korean J Anim Sci. 1998 ; 14(1) : 93-6.
  9. Na KJ, Kang HY, Yoon SK, Jeung EB. Biological efficacy of essential oils from softwood. Korean J Lab Anim Sci. 1999 ; 15(1) : 79-81.
  10. Lee HO, Baek SH, Han DM. Antimicrobial Effects of Chamaecyparis obtusa Essential Oil. Kor J Appl Microbiol Biotechnol. 2001 ; 29 : 253-7.
  11. Cowan MM. Plant products as antimcrobial agents. Clin Microbiol Rev. 1999 ; 12 : 564-82
  12. Hong EJ, Na KJ, Choi IG, Choi KC, Jeung EB. Antibacterial and antifungal effects of essential oils from coniferous trees. Biol Pharm Bull. 2004 ; 27 : 863-6. https://doi.org/10.1248/bpb.27.863
  13. Joo SS, Yoo YM, Ko SH, Choj W, Park MJ, Kang HY, Choi KC, Choi IG, Jeung EB. Effects of essential oil from Chamaecypris obtusa on the development of atopic dermatitis-like skin lesions and the suppression of Th cytokines. Journal of Dermatological Science. 2010 ; 60 : 120-9. https://doi.org/10.1016/j.jdermsci.2010.08.007
  14. Choi HJ, Sim BY, Miyamoto T. Effects of Lobophytum crassum extract(MC-1) on Various Immunological Factors Related to Pathogenesis of Atopic Dermatitis in Dermatophagoides Pteronyssinus Treated NC/Nga mice. Kor J Herbology. 2015 ; 30(1) : 95-101. https://doi.org/10.6116/KJH.2015.30.1.95.
  15. Vestergaard C, Yoneyama H, Matsushima K. The NC/Nga mouse: a model for atopic dermatitis. Mol Med Tody. 2000 ; 6 : 209-10. https://doi.org/10.1016/S1357-4310(00)01683-X
  16. Vestergaard C, Yoneyama H, Murai M, Nakamura K, Tamaki K, Terashima Y, Imai T, Yoshie O, Irimura T, Mizutani H, Matsushima K. Overproduction of Th2-specific chemokines in NC/Nga mice exhibiting atopic dermatitis-like lesions. J Clin Invest. 1999 ; 104 : 1097-105. https://doi.org/10.1172/JCI7613
  17. Hashimoto Y, Arai I, Nakanishi Y, Sakurai T, Nakamura A, Nakaike S. Scratching of their skin by NC/Nga mice leads to development of dermatitis. Life Sciences. 2004 ; 76 : 783-94. https://doi.org/10.1016/j.lfs.2004.07.022
  18. Kang JS, Lee K, Han SB, Ahn JM, Lee H, Han MH, Yoon YD, Yoon WK, Park SK, Kim HM. Induction of atopic eczema/dermatitis syndromelike skin lesions by repeated topical application of a crude extract of Dermatophagoides pteronyssinus in NC/Nga mice. Int Immunopharmacol. 2006 ; 6 : 1616-22. https://doi.org/10.1016/j.intimp.2006.06.011
  19. Buske-Kirschbaum A, Gierens A, Hollig H, Hellhammer DH, Hollig H. Stress-induced immunomodulation is altered in patients with atopic dermatitis. J Neuroimmunol. 2002 ; 129(1-2) : 161-7. https://doi.org/10.1016/S0165-5728(02)00168-6
  20. Raap U, Werfel T, Jaeger B, Schmid-Ott G. Atopic dermatitis and psychological stress. Hautarzt. 2003 ; 54(10) : 925-9. https://doi.org/10.1007/s00105-003-0609-z
  21. Hoglund C, Axen J, Kemi C, Jernelov S, Grunewald J, Muller-Suur C, Smith Y, Gronneberg R, Eklund A, Stierna P, Lekander M. Changes in immune regulation in response to examination stress in atopic and healthy individuals. Clin Exp Allergy. 2006 ; 36(8) : 969-71. https://doi.org/10.1111/j.1365-2222.2006.02538.x
  22. Cole, A., & Shanley, E. Complementary therapies as a means of developing the scope of professional nursing practice. Journal of Advanced Nursing. 1998 ; 27 (6) : 1171-6. https://doi.org/10.1046/j.1365-2648.1998.00643.x
  23. Han SG, Perfume and Fragrance. Seoul : Shinkwang. 2001 : 245-64.