The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Antioxidant Activities of Hot Water and Ethanol Extracts from the stem of Sorbus commixta Heal

마가목 줄기 열수 및 에탄올 추출물의 항산화 활성

  • Yoo, Ji-Hyun (Department of Herbal pharmaceutical Science, Joongbu University) ;
  • Doh, Eun-Soo (Department of Herbal pharmaceutical Science, Joongbu University) ;
  • Chang, Jun-Pok (Department of Herbal pharmaceutical Science, Joongbu University) ;
  • Kil, Ki-Jung (Department of Herbal pharmaceutical Science, Joongbu University)
  • 유지현 (중부대학교 한방제약과학과) ;
  • 도은수 (중부대학교 한방제약과학과) ;
  • 장준복 (중부대학교 한방제약과학과) ;
  • 길기정 (중부대학교 한방제약과학과)
  • Received : 2017.04.11
  • Accepted : 2017.05.20
  • Published : 2017.05.30
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Abstract

Objectives : In order to investigate the possibility of Sorbus commixta Heal. stem (SC) as a natural material, antioxidant activities of the hot water and ethanol extracts were examined. Methods : The samples of SC were pulverized, and fractions were extracted repeatedly three times from hot water and 70% ethanol at room temperature for 2 hours. The antioxidant activities were analyzed from total polyphenol, flavonoid contents, DPPH, ABTS, hydroxyl radical, $Fe2^+$ chelating, and nitrite scavenging activity. Results : Total polyphenol contents were significantly higher in ethanol extract group ($504.39{\mu}g/m{\ell}$) than in hot water extract group ($364.64{\mu}g/m{\ell}$). Total flavonoid contents were also significantly higher in ethanol extract group ($160.09{\mu}g/m{\ell}$) than in hot water extract group ($124.59{\mu}g/m{\ell}$). DPPH, ABTS, $Fe2^+$ chelating were slightly higher in ethanol extract gorups than in hot water extract groups, and increased in a dose-dependent manner. The hydroxyl radical scavenging activity (18.42~23.61%) of ethanol extract groups were shown to be approximately twice higher than that (7.63~10.37%) of hot water extract groups at $12.5{\sim}50{\mu}g/m{\ell}$ concentration. Nitrite scavenging activities of both ethanol and hot-water extract groups were shown to be higher in a dose dependent manner at the concentration of $12.5{\sim}50{\mu}g/m{\ell}$ at pH 3.0 than at pH 1.2, and ethanol extract groups (86.55~96.64%) had higher activity than the hot water extract groups (42.59~92.63%), which was higher than that of the control group antioxidant BHT (72.96~80.11%). Conclusions : The extracts of SC displayed antioxidant activities which suggested a natural material can be developed to functional material.

Keywords

References

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