The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Inhibition of Inflammation by Kyeongok-go with Black ginseng in LPS-induced RAW 264.7 Macrophages

LPS로 염증유도된 RAW 264.74 세포에 대한 흑삼 첨가 경옥고의 항염증 효과

  • Park, MyungJae (College of Korean Medicine, Daejeon University) ;
  • Kim, Jeong-Soo (College of Korean Medicine, Daejeon University) ;
  • Lee, AhReum (College of Korean Medicine, Daegu Haany University) ;
  • Roh, Seong-Soo (College of Korean Medicine, Daegu Haany University) ;
  • Kwon, OJun (Gyeongbuk Institute for Regional Program Evaluation) ;
  • Seo, Young-Bae (College of Korean Medicine, Daejeon University)
  • 박명재 (대전대학교 한의과대학 본초학교실) ;
  • 김정수 (대전대학교 한의과대학 본초학교실) ;
  • 이아름 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 권오준 (경북지역사업평가단) ;
  • 서영배 (대전대학교 한의과대학 본초학교실)
  • Received : 2017.04.11
  • Accepted : 2017.05.20
  • Published : 2017.05.30
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Abstract

Objectives : The aim of this study was to examine effect of anti-oxidant and anti-inflammation activity of the Kyeongok-go with various processing methods that was manufactured by heating mantle. Methods : Commercial Kyeongok-go (K0) was purchased and Kyeongok-go with ginseng (K1), Kyeongok-go with black ginseng (BK), ginseng fermentation Kyeongok-go (KF), black ginseng fermentation Kyeongok-go (BKF) were manufactured by heating mantle. To examine anti-oxidant effect, DPPH radical and production of NO and ROS in RAW 264.74 cell were used. Furthermore, to determined anti-inflammation effect, measured pro-inflammatory mRNA such as NOS-II, COX-2, $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ in RAW 264.74 cell treated with K0, K1, KF, BK, and BKF. Result : K1 scavenged DPPH radical effectively than K0. The most DPPH radical scavenging activity was BKF. In the RAW 264.74 cells stimulated with LPS, NO and ROS production were measured. As a results, K1 was decreased NO, ROS production compared with K0, and BKF was reduced similarly to cyclosporine A (positive control). Expression of pro-inflammatory mRNA such as NOS-II, COX-2, $IL-1{\beta}$, IL-6 showed a significant decrease in BK or BKF. But, there was no significant in expression of $TNF-{\alpha}$ in all extract treatmetn groups. Conclusions : According to the above results, it is considered that Kyeongok-go with fermented black ginseng (BKF) manufactured by heating mantle is effective material that have anti-inflammation and anti-oxidant activities. Our finding indicate that BKF may be an effective agent for anti-inflammation through anti-oxidant effect.

Keywords

References

  1. Li JW. Dictionary of Chinese medicine. Beijing : People's Health Publishing House. 2004 : 1669.
  2. Heo J. Donguibogam naegyeongpyeon. Seoul : Gyemyeong Publishing House. 1993 : 78.
  3. Hwang DY, Daeyeog dongmaeg bangyaghabpyeon. Seoul : Namsandang. 1977 : 191.
  4. Shin BY. Anti-amnesic effects of a herbal medicinal prescription modified from Kyung-Ok-Ko on Scopolamine- or transient forebrain Ischemia-Induced Memory Impairment. Kyung Hee University. 2011 : 1-53.
  5. Kim JB, Song HN. Effects of Kyeongok-go and Its Two Added Precriptions on Hyperlipidemic Rats Induced by High-fat Diet. Korean J. Oriental Physiology & Pathology. 2014 ; 28(4) : 371-8.
  6. Jung HJ, Jung SK, Rhee HK, Jeon SB. Experimental Studies of the effects of Kyungok-go aganinst Mycobacteria tuberculosis. Korean J. Orient Int. Med. 2000 ; 21(4) : 555-63.
  7. Lee KS, Kim GH, Kim HH, Seong BJ, Kim SI, Han SH, Kang EJ, Yoo YC. Qualities and Anti-inflammatory Activity of Kyungokgos Sold in Local Markets. J Korean Soc Food Sci Nutr. 2013 ; 42(3) : 335-41. https://doi.org/10.3746/jkfn.2013.42.3.335
  8. Lee SY, Shin YJ, Park JH, Kim SM, Park CS. An Analysis of the Gyungokgo's Ingredients and a Comparison Study on Anti-oxidation Effects According to the Kinds of Extract. Kor. J. Herbology. 2008 ; 23(2) : 123-36.
  9. Whang WK, Oh IS, Kim YB, Shin SD, Kim IH. The Physiological Activities of KYUNG OK-KO(III)-Effects on Inflammation, Gastric Ulcer, Analgesic and Homothermics. Kor. J. Pharmacogn. 1994 ; 25(2) : 153-9.
  10. Kim JH. The Antifatigue effect of modified kyungohkgo composition oriental medicines, Wonkwang University. 2011 : 1-35.
  11. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature, 1958 ; 181(4617) : 1199-1200. https://doi.org/10.1038/1811199a0
  12. Lee JH, Seo YB, Kim BS. A study of modern application of Kyungohkgo through historical analysis of its virtues. Kor. J. Oriental Medicine Institute. 2016 : 24(2) : 25-34.
  13. Kwon SU, Lee HY, Xin MG, Il JY, Cha JY, Kim HJ, Kwak WR, Go CK, Kim DK, Lee YM. Preparation of Kyungohkgo suspension beverage contating cultivated wild ginseng. Korean. J. Oriental Physiology & Pathology. 2013 ; 27(2) : 233-8.
  14. Ann SW, Kim YC, Hwang IS, Cho JW, Kim MS, Lee JK, Eum WY. Effect of seafood amino acid fertilizer and Korean effective microorganisms on the fruit quality of fuji apple. Jounal of Enviornmental Science International. 2010 ; 19(10) : 1293-99.
  15. Doh ES, Chang JP, Lee KH, Seong NS. Ginsenoside Change and Antioxidation Activity of Fermented Ginseng. Korean J. Medicinal Crop Sci. 2010 ; 18(4) : 255-65.
  16. Lee ST, Jeong YR, Ha MH, Kim SH, Byun MW, Jo SK. Induction of nitric oxide and TNF-a by herbal plant extract in mouse macrophage. J. Korean Soc. Food Sci. Nutr. 2000 ; 29(2) : 342-8.
  17. Jeong JB, Hong SC, Jeong HJ, Koo JS, Antiinflammatory effects of ethyl acetate fraction from Cnidium officinale Makino on LPS-stimulated RAW 264.74 and THP-1 cells. Korean J. Plant Res. 2012 ; 25(3) : 299-307. https://doi.org/10.7732/kjpr.2012.25.3.299
  18. Kim DH, Park SJ, Jung JY, Kim SC, Byun SH, Anti-inflammatory effects of the aqueous extract of Hwangnyenhaedoktang in LPS-activated macrophage cells. Kor. J. Herbology. 2009 ; 24(4) : 39-47.
  19. Sherwood L. Fundamentals of Human Physiology. Massachusetts : Cengage Learning Publishing. 2011 : 35.
  20. Jang AY, Sueng YC, Ji JG. The comparative study on physiological activity of White ginseng, Red ginseng and Black ginseng extract. Journal of Digital Concergence. 2016 ; 14(5) : 459-71.
  21. Shin SH, Shin YO, Lee JY, Lee AR, Kim MY, Park CH, Seo BI, Roh SS. Ethanol-Heated Processed Scutellariae Radix Improve Inflammatory Response through an Inhibitory Effect against Oxidative Stress in Mice with the Lipopolysaccharide-induced Intestine Injury of Mice. Kor. J. Herbology. 2015 ; 30(4) : 81-8. https://doi.org/10.6116/kjh.2015.30.4.81.
  22. Lee EI, Oh EH, Song HJ, Choi WJ, Baek JO, Lee JR, Roh JY. Oxidative Damage to Macromolecules in Atopic Dermatitis Patients. Kor. J. dermatology. 2015 ; 53(6) : 456-61.
  23. Lawrence T, Willoughby DA, Gilroy DW. Antiinflammatory lipid mediators and insights into the resolution of inflammation. Nat Rev Immunol 2002 ; 2(10) : 787-95. https://doi.org/10.1038/nri915
  24. Xie QW, Whisnant R, Nathan C, Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon gamma and bacterial lipopolysaccharide. J Exp Med. 1993 ; 177(6) : 1779-84. https://doi.org/10.1084/jem.177.6.1779