The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Effects of Hot Water Extracts from Lonicera Japonica Flos Extracts on whitening using B16F10 cell lines

금은화(金銀花) 열수 추출물이 피부 미백(美白)에 미치는 영향

  • Yu, Pei-Yen (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University) ;
  • Kim, Hye-Hwa (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University) ;
  • Lee, Yu-Lim (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University) ;
  • Park, Soo-Yeon (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University) ;
  • Jung, Min-Yeong (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University) ;
  • Choi, Jung-Hwa (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University)
  • 우배언 (동신대학교 한의과대학 한방안이비인후피부과교실) ;
  • 김혜화 (동신대학교 한의과대학 한방안이비인후피부과교실) ;
  • 이유림 (동신대학교 한의과대학 한방안이비인후피부과교실) ;
  • 박수연 (동신대학교 한의과대학 한방안이비인후피부과교실) ;
  • 정민영 (동신대학교 한의과대학 한방안이비인후피부과교실) ;
  • 최정화 (동신대학교 한의과대학 한방안이비인후피부과교실)
  • Received : 2017.04.03
  • Accepted : 2017.05.15
  • Published : 2017.05.25
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Abstract

Objectives : This study was designed to investigate effects of Lonicera Flos Extracts(LFE) on whitening using B16F10 cell lines. Methods : In this experiment, we observed effects of LFE on cell viability, inhibitory effects of melanin synthesis, inhibitory effect on tyrosinase, tyrosinase activity, superoxide dismutase(SOD)-like activity and mRNA expression. Results : In results, more than $2000{\mu}g/ml$ of LFE treated group showed lowered cell viability rates significantly compared to albutin treated group. More than $2000{\mu}g/ml$ of LFE treated groups were lower levels of melanin synthesis. Inhibitory effects of melanin production showed in $1000{\mu}g/ml$ of LFE treated group. $1,000{\mu}g/ml$ of LFE treated group significantly suppressed tyrosinase activities in vitro. LFE and albutin treated group significantly decreased tyrosinase activity compared to non treated group. SOD-like activity of LFE treated group was lower than vitamin C treated group but increased depending on concentration. $500{\mu}g/ml$ of LFE treated group and $1,000{\mu}g/ml$ of LFE treated group was significantly increased. Tyrosinase mRNA expression of ${\alpha}$-MSH and LFE $250{\mu}g/ml$ treated group significantly decreased compared to ${\alpha}$-MSH treated group. Conclusions : These results suggest that LFE can inhibit melanin synthesis through inhibitory action on tyrosinase activity. And LFE suppressed tyrosinase activities B16F10 cells significantly. So I suggest LFE can apply to whitening.

Keywords

References

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