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http://dx.doi.org/10.6114/jkood.2017.30.2.038

Effects of Hot Water Extracts from Lonicera Japonica Flos Extracts on whitening using B16F10 cell lines  

Yu, Pei-Yen (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University)
Kim, Hye-Hwa (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University)
Lee, Yu-Lim (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University)
Park, Soo-Yeon (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University)
Jung, Min-Yeong (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University)
Choi, Jung-Hwa (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongsin University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.30, no.2, 2017 , pp. 38-50 More about this Journal
Abstract
Objectives : This study was designed to investigate effects of Lonicera Flos Extracts(LFE) on whitening using B16F10 cell lines. Methods : In this experiment, we observed effects of LFE on cell viability, inhibitory effects of melanin synthesis, inhibitory effect on tyrosinase, tyrosinase activity, superoxide dismutase(SOD)-like activity and mRNA expression. Results : In results, more than $2000{\mu}g/ml$ of LFE treated group showed lowered cell viability rates significantly compared to albutin treated group. More than $2000{\mu}g/ml$ of LFE treated groups were lower levels of melanin synthesis. Inhibitory effects of melanin production showed in $1000{\mu}g/ml$ of LFE treated group. $1,000{\mu}g/ml$ of LFE treated group significantly suppressed tyrosinase activities in vitro. LFE and albutin treated group significantly decreased tyrosinase activity compared to non treated group. SOD-like activity of LFE treated group was lower than vitamin C treated group but increased depending on concentration. $500{\mu}g/ml$ of LFE treated group and $1,000{\mu}g/ml$ of LFE treated group was significantly increased. Tyrosinase mRNA expression of ${\alpha}$-MSH and LFE $250{\mu}g/ml$ treated group significantly decreased compared to ${\alpha}$-MSH treated group. Conclusions : These results suggest that LFE can inhibit melanin synthesis through inhibitory action on tyrosinase activity. And LFE suppressed tyrosinase activities B16F10 cells significantly. So I suggest LFE can apply to whitening.
Keywords
Whitening; Lonicerae Flos Extracts; LFE; B16F10 cells;
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Times Cited By KSCI : 8  (Citation Analysis)
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