Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Establishment of Pre-Harvest Residue Limits and Residue Characteristics of Penthiopyrad and Pyriofenone in Cucumber (Cucumis sativus L.) Under Greenhouse Condition

시설 내 오이(Cucumis sativus L.) Penthiopyrad 재배 중 및 Pyriofenone의 잔류특성과 생산단계 잔류허용기준 설정

  • Leem, Su-Bin (School of Natural Resources and Environmental Science, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Kim, Ji-Yoon (School of Natural Resources and Environmental Science, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Hur, Kyung-Jin (School of Natural Resources and Environmental Science, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Kim, Hee-Gon (School of Natural Resources and Environmental Science, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Hur, Jang-Hyun (School of Natural Resources and Environmental Science, College of Agriculture and Life Sciences, Kangwon National University)
  • 임수빈 (강원대학교 농업생명과학대학 환경융합학부) ;
  • 김지윤 (강원대학교 농업생명과학대학 환경융합학부) ;
  • 허경진 (강원대학교 농업생명과학대학 환경융합학부) ;
  • 김희곤 (강원대학교 농업생명과학대학 환경융합학부) ;
  • 허장현 (강원대학교 농업생명과학대학 환경융합학부)
  • Received : 2017.02.10
  • Accepted : 2017.03.24
  • Published : 2017.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

BACKGROUND: Greenhouse crops are one of agricultural products consumed largely in Korea. Cucumber is a typical example as main vegetables of greenhouse crops. Thus, pesticide residue analysis is an important requirement to guarantee pesticide safety of cucumber. This work was aim to investigate the residues of penthiopyrad and pyriofenone in cucumber after harvest. METHODS AND RESULTS: Cucumber was subjected to treat with penthiopyrad and pyriofenone at a level of recommended dose 0, 1, 2, 3, 5, 7 and 10 days before harvest under greenhouse conditions. The samples were extracted with organic solvent by using a homogenizer and purified on solid phase cartridge column followed by LC-MS/MS analysis. The recovery levels of penthiopyrad and pyriofenoneranged from approximately 81 to 93% with the method limit of 0.005 mg/kg and coefficient of variation less than 10%. Penthiopyrad and pyriofenone were detected at a level less than maximum residue limit in cucumber at 10 days before. The half-lives of penthiopyrad and pyriofenone were determined to 2.4 ~ 2.6 days. CONCLUSION: Penthiopyrad and pyriofenone are suggested to use in cucumber 10 days before harvest to reach their levels less than maximum residue limit.

본 연구는 시설재배 작물인 오이의 생산단계 중 penthiopyrad 및 pyriofenone의 시간경과에 따른 잔류량을 감소식으로 회귀식을 계산한 후, 생물학적 반감기를 산출함으로써 생산단계 잔류허용기준을 설정하여 농가의 안전한 농산물생산에 기여하고자 수행하였다. Penthiopyrad 및 pyriofenone는 작물보호지침서의 안전사용기준에 준하여 기준량 1회 처리하였고, 각각 포장 1과 포장 2 지역으로 나누어 처리하였다. 약제살포 2시간 후를 0일차로 하여 0, 1, 2, 3, 5, 7, 10일차에 시료를 채취하였다. 두 약제 모두 추출 및 정제를 위해 acetonitrile, dichloromethane, SPE $NH_2$ cartridge 를 사용하였고 LC-MS/MS를 이용하여 분석하였으며, 분석 정량한계는 0.005 mg/kg이었다. 분석정량한계의 10배, 50배 수준으로 농약을 처리하여 수행한 결과 penthiopyrad 및 pyriofenone의 회수율은 각각 92.8~.95.4%, 81.0~89.8%이었으며, 표준편차는 두 약제 모두 10% 미만이었다. 오이 중 두 약제에 대한 포장 1, 포장 2의 생물학적 반감기는 penthiopyrad의 경우 각각 2.6일과 2.5일, pyriofenone의 경우 각각 2.5일과 2.4일로 나타났다. Penthiopyrad 및 pyriofenone의 잔류량을 바탕으로 오이 수확 10일 전 잔류량이 3.44 mg/kg, 4.63 mg/kg이하로 나타난다면 수확 시 두 약제의 잔류량은 MRL (Penthiopyrad: 0.5 mg/kg, Pyriofenone: 0.7 mg/kg) 수준 이하일 것으로 사료된다. 본 연구결과를 바탕으로 오이 중 penthiopyrad 및 pyriofenone의 생산단계 잔류허용기준이 설정된다면 오이 재배농가에서 유통단계 뿐만 아니라 생산단계에서 안전한 농산물을 생산하는데 기여할 것으로 사료된다.

Keywords

References

  1. Ha, H. Y., Ra, D. S., Shin, W. C., Im, G. J., & Park, J. E. (2012). Survey of pesticide use in fruit vegetables, fruits, and rice cultivation areas in Korea. The Korean Journal of Pesticide Science, 16(4), 395-400. https://doi.org/10.7585/kjps.2012.16.4.395
  2. Han, Y. H., Lee. C. Y., Park. K. D., Park. K. W. & Lee. K. S. (2013). Residues of new fungicide, isopyrazam on cucumber and oriental melon. Korean Jounal of Pesticide Science, 17(1), 13-19. https://doi.org/10.7585/kjps.2013.17.1.13
  3. Hwang, K. W., Kim, T. W., Yoo, J. H., Park, B. S., & Moon, J. K. (2012). Dissipation pattern of amisulbrom in cucumber under greenhouse condition for establishing pre-harvest residue limit. The Korean Journal of Pesticide Science, 16(4), 288-293. https://doi.org/10.7585/kjps.2012.16.4.288
  4. Kanazawa, J. (1992). Environmental Sciences of Pesticide, pp.55-56, Cooperative Publication, Japan.
  5. Kim, K. J., Kim, D. S., Heo, S. J., Ham, H. J., &Hur, J. H. (2013). Establishment of pre-harvest residue limit (PHRL) of emamectin benzoate during cultivation of amaranth. The Korean Journal of Pesticide Science, 17(2), 77-83. https://doi.org/10.7585/kjps.2013.17.2.77
  6. Kim, Y. G. & Park. C. K. (2002). Control technology of pest in cucumber for exportance. The Korean Society for Bio-Environment Control Simpojium and other publications, pp. 61-82.
  7. Kim, Y. S., Park, J. H., Park, J. W., Lee, Y. D., Lee, K. S., & Kim, J. E. (2002). Persistence and Dislodgeable Residues of Chlorpyrifos and Procymidone in Lettuce Leaves under Greenhouse Condition. Korean Journal of Environmental Agriculture, 21(2), 149-155. https://doi.org/10.5338/KJEA.2002.21.2.149
  8. Kim, Y. S., Park, J. H., Park, J. W., Lee, Y. D., Lee, K. S., & Kim, J. E. (2003). Residue levels of chlorpyrifos and chlorothalonil in apples at harvest. Korean Journal of Environmental Agriculture, 22(2), 130-136. https://doi.org/10.5338/KJEA.2003.22.2.130
  9. Korea Crop Protection Association (2015). 2015 Guideline of test for pesticide registration, pp. 87-318.
  10. Lee, E. Y., Noh, H. H., Park, Y. S., Kang, K. W., Lee, K. H., Park, H. K., Yun. S. S., Jin. C. W., Han. S. K. & Kyung, K. S. (2009). Residual characteristics of Neonicotinoid Insecticide dinotefuran and thiacloprid in cucumber. The Korean Journal of Pesticide Science, 13(2), 98-104.
  11. Lee, H. D., Ihm. Y. B., Kwon. H. Y., Kim. J. B., Kyung. K. S., Park. S. S., Oh. B. Y., Im. G. J. & Kim. J. E. (2005). Characteristics of Pesticide residue in/on cucurbitaceous fruit vegetables applied with foliar spraying under greenhouse. The Korean Society of Pesticide Science, 9(4), 359-364.
  12. Lee, H. D., Kyung, K. S., Kwon, H. Y., Ihm, Y. B., Kim, J. B., Park, S. S., & Kim, J. E. (2004). Residue characteristics of hexaconazole and chlorothalonil in several fruits. The Korean Journal of Pesticide Science, 8(2), 107-111.
  13. Lee, M. G., Hwang, J. M., & Lee, S. R. (2005). The usage status of pesticides for vegetables under greenhouse cultivation in the southern area of Korea. The Korean Journal of Pesticide Science, 9(4), 391-400.
  14. Lee, J. H., Park, H. W., Keum, Y. S., Kwon, C. H., Lee, Y. D., & Kim, J. H. (2008). Dissipation pattern of boscalid in cucumber under greenhouse condition. The Korean Journal of Pesticide Science, 12(1), 67-73.
  15. Lee, J. H., Jeon, Y. H., Shin, K. S., Kim, H. Y., Park, E. J., Kim, T. H., & Kim, J. E. (2009). Biological half-lives of fungicides in Korean melon under greenhouse condition. Korean Journal of Environmental Agriculture, 28(4), 419-426. https://doi.org/10.5338/KJEA.2009.28.4.419
  16. Lee, Y. J., Go, G. Y., Won, D. J., Gil, G. H. and Lee, G. S. (2003) Residue Patterns of Procymidone, Chlorpyrifos and Cypermethrin in Peaches During Cultivation and Storage Period. Korean Journal of Environmental Agriculture, 22(3), 220-226. https://doi.org/10.5338/KJEA.2003.22.3.220
  17. Moon. S. H., Kwon. H. Y., Hong. S. M., Kim. S. S., Son K. A. & Lim. C. H. (2016). Comparison of pesticide residue amounts in apple trees applied by different sprayers and spray volumes. The Korean Society of Pesticide Science, 20(3), 264-270. https://doi.org/10.7585/kjps.2016.20.3.264
  18. Nam, M. H., Chio. J. P., Kim. H. J., Lee. J. J., Lim. K. H., Kim. Y. G., Kim. H. T. & Jeun. Y. C. (2010). Controlling activity of Bacillus subtilis KB-401 against cucumber powdery mildew caused by Sphaerotheca fusca. Korean Jounal of Pesticide Science, 14(1), 49-53.
  19. Park, D. S., Seong, K. Y., Choi, K. I., & Hur, J. H. (2005). Field tolerance of pesticides in the strawberry and comparison of biological half-lives estimated from kinetic models. The Korean Journal of Pesticide Science, 9(3), 231-236.
  20. Park. H. K., Noh. H. H., Lee. G. H., Lee. J. Y., Park. Y. S., Kang. K. W., Lee. E. Y., Yun. S. S., Jin. C.W. &Kyung. K. S. (2011). Residual characteristic of chlorpyrifos in squash and estimation of its residues before harvest. The Korean Journal of Pesticide Science. 15(4), 463-470.
  21. Turner, J. A. (2015). The Pesticide Manual: A World Compendium. pp.855-984, British Crop Protection Council, UK.