한국환경농학회지 (Korean Journal of Environmental Agriculture)

  • 제36권1호
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  • Pages.36-42
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  • 2017
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  • 1225-3537(pISSN)
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  • 2233-4173(eISSN)
한국환경농학회 (The Korean Society of Environmental Agriculture)
권호 표지
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Pseudomonas sp. EP-3 rhamnolipid 에 의한 진딧물 살충성 생산을 위한 유자씨앗 부산물의 재활용

Reuse of Yuza Seed By-product for Production of Aphicidal Rhamnolipid by Pseudomonas sp. EP-3

  • 임다정 (전남대학교 농화학과, 친환경농업연구소) ;
  • 박태현 (LG 팜한농(주)) ;
  • 양시영 (전남대학교 농화학과, 친환경농업연구소) ;
  • 김진철 (전남대학교 농화학과, 친환경농업연구소) ;
  • 김인선 (전남대학교 농화학과, 친환경농업연구소)
  • Lim, Da Jung (Department of Agriculture chemistry, Institute of Environmentally Friendly Agriculture, Chonnam National University) ;
  • Park, Tae Hyun (Crop Protection R&D Center, LG Farmhannong) ;
  • Yang, Si Young (Department of Agriculture chemistry, Institute of Environmentally Friendly Agriculture, Chonnam National University) ;
  • Kim, Jin Cheol (Department of Agriculture chemistry, Institute of Environmentally Friendly Agriculture, Chonnam National University) ;
  • Kim, In Seon (Department of Agriculture chemistry, Institute of Environmentally Friendly Agriculture, Chonnam National University)
  • 투고 : 2017.02.09
  • 심사 : 2017.03.24
  • 발행 : 2017.03.31
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초록

BACKGROUND: Yuza seed by-product has been produced in a large amount from the agricultural farms in the southern area of Korea. It has been mostly abandoned after commercial process for the production of juice, jam and tea. The study on the reuse of the yuza seed by-product has received much attention as a bio-resource material for the production of active compound in agriculture. METHODS AND RESULTS: Insecticidal rhamnolipid-producing Pseudomonas sp. EP-3 was grown in mineral salt media with the yuza seed by-product at 2, 20, 50 and 100 g/L. The growth of EP-3 was accompanied by a increase in insecticidal activity against green peach aphid. The highest insecticidal activity was observed when EP-3 was grown in the medium containing 50 g/L of the seed sample, producing approximately 996 mg/L of rhamnolipid at 96 h. Palmitic acid, stearic acid, oleic acid and linoleic acid were determined as the major fatty acids of the seed sample. The EP-3 cultures grown on the fatty acid mixture extracted from the seed sample showed a aphid mortality similar to that of cultures grown on the seed sample. The EP-3 cultures grown on 50 g/L of the seed sample showed aphid mortality more than 90% under greenhouse conditions. CONCLUSION: This study suggested that the yuza seed by-product may be used as a renewable material for microbial production of rhamnolipid against green peach aphid.

키워드

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