The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Tribuli Fructus extract on inflammatory responses in IgE-stimulated RBL-2H3 mast cells

비만세포에서 백질려 추출물의 항염증효과에 대한 연구

  • Rho, Hyo Sun (Department of Herblolgy, college of Korean Medicine, Dongguk University) ;
  • Park, Yong-Ki (Department of Herblolgy, college of Korean Medicine, Dongguk University) ;
  • Bae, Hyo Sang (Department of Sasang Constitutional Medicine, college of Korean Medicine, Dongguk University)
  • 노효선 (동국대학교 한의과대학 본초학교실) ;
  • 박용기 (동국대학교 한의과대학 본초학교실) ;
  • 배효상 (동국대학교 한의과대학 사상체질과)
  • Received : 2017.02.14
  • Accepted : 2017.03.15
  • Published : 2017.03.30
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Abstract

Objectives : Tribulus terrestris $Linn{\acute{e}}$ (Tribuli Fructus; TF) has been used to treat hypochondrium, agalactia, nebula, itching and vitiligo in traditional Korean medicine. In this study, we investigated the effects of TF 30% ethanol extract on inflammatory responses in IgE-stimulated RBL-2H3 mast cells. Methods : TF extract was prepared by 30% ethanol. RBL-2H3 cells, a rat mast cell line, were treated with TF extract at different concentrations for 1 hr and then stimulated with DNP-IgE/HSA for indicated times. Cell viability was measured by WST-1 assay. The expression of inflammatory cytokines (IL-4, IL-13 and $IFN-{\gamma}$) mRNA was determined by reverse transcriptase-PCR, and the phosphorylation of ERK1/2, p38 and JNK MAP kinases (MAPKs) was determined by Western blot. The nuclear expression of $NF-{\kappa}B$ p65 in the cells was detected by Western blot and immunocytochemistry, respectively. Results : The treatment of TF extract at 0.1 and $0.2mg/m{\ell}$ significantly decreased the expression of IL-4 and IL-13 mRNA in IgE-stimulated RBL-2H3 mast cells, while significantly increased the expression of $IFN-{\gamma}$ mRNA. TF extract treatment was also inhibited the phosphorylation of ERK1/2, p38 and JNK MAPKs in IgE-stimulated RBL-2H3 mast cells in a dose-dependent manner. In addition, TF extract significantly blocked the translocation of $NF-{\kappa}B$ p65 into the nuclear of cells after IgE stimulation. Conclusions : These results indicate that TF extract inhibits inflammatory response in IgE-stimulated mast cells through blocking MAPKs/$NF-{\kappa}B$ pathway. This suggests that TF extract has an anti-inflammatory activity in mast cell activation.

Keywords

References

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