Browse > Article
http://dx.doi.org/10.6116/kjh.2017.32.2.107.

Effects of Tribuli Fructus extract on inflammatory responses in IgE-stimulated RBL-2H3 mast cells  

Rho, Hyo Sun (Department of Herblolgy, college of Korean Medicine, Dongguk University)
Park, Yong-Ki (Department of Herblolgy, college of Korean Medicine, Dongguk University)
Bae, Hyo Sang (Department of Sasang Constitutional Medicine, college of Korean Medicine, Dongguk University)
Publication Information
The Korea Journal of Herbology / v.32, no.2, 2017 , pp. 107-114 More about this Journal
Abstract
Objectives : Tribulus terrestris $Linn{\acute{e}}$ (Tribuli Fructus; TF) has been used to treat hypochondrium, agalactia, nebula, itching and vitiligo in traditional Korean medicine. In this study, we investigated the effects of TF 30% ethanol extract on inflammatory responses in IgE-stimulated RBL-2H3 mast cells. Methods : TF extract was prepared by 30% ethanol. RBL-2H3 cells, a rat mast cell line, were treated with TF extract at different concentrations for 1 hr and then stimulated with DNP-IgE/HSA for indicated times. Cell viability was measured by WST-1 assay. The expression of inflammatory cytokines (IL-4, IL-13 and $IFN-{\gamma}$) mRNA was determined by reverse transcriptase-PCR, and the phosphorylation of ERK1/2, p38 and JNK MAP kinases (MAPKs) was determined by Western blot. The nuclear expression of $NF-{\kappa}B$ p65 in the cells was detected by Western blot and immunocytochemistry, respectively. Results : The treatment of TF extract at 0.1 and $0.2mg/m{\ell}$ significantly decreased the expression of IL-4 and IL-13 mRNA in IgE-stimulated RBL-2H3 mast cells, while significantly increased the expression of $IFN-{\gamma}$ mRNA. TF extract treatment was also inhibited the phosphorylation of ERK1/2, p38 and JNK MAPKs in IgE-stimulated RBL-2H3 mast cells in a dose-dependent manner. In addition, TF extract significantly blocked the translocation of $NF-{\kappa}B$ p65 into the nuclear of cells after IgE stimulation. Conclusions : These results indicate that TF extract inhibits inflammatory response in IgE-stimulated mast cells through blocking MAPKs/$NF-{\kappa}B$ pathway. This suggests that TF extract has an anti-inflammatory activity in mast cell activation.
Keywords
Tribulus terrestris $Linn{\acute{e}}$; Tribuli Fructus; RBL-2H3 cells; inflammatory responses; MAPKs/$NF-{\kappa}B$ pathway;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Jane W. IMMUNOBIOLOGY-6/E : The Immune System In Health And Disease. Seoul : LifeScience. 2005 : 361, 551-94.
2 Eajakawa S. Skin diseases of children. Seoul : Korea Medical Book Publishig Company. 2005 : 20-30.
3 Rhim JW, Moon KS, Kong DY, Pyun BY. An Investigation into the Actual Condition of Outbreak and Treatment in Atopic Dermatitis. Allergy Asthma Respir Dis. 2005 ; 15(1) : 44-52.
4 Hong CY. Pediatrics. Seoul : Daehan Printing & Publishing Co. 2005 : 1207-11.
5 Rha YH, Choi SH. Editorial : Risk Factors Associated with Development of Atopic Dermatitis. Pediatr Allergy Respir Dis. 2009 ; 19(2) : 91-4.
6 Park YM. Advances In the Pathophysiology of Atopic Dermatitis. Pediatr Allergy Respir Dis. 2006 ; 16(3) : 189-96.
7 Boguniewicz M, Leung DYM. Atopic dermatitis. J Allergy Clin Immunol. 2006 ; 117(Suppl) : 475-80.   DOI
8 Buske-Kirschbaum A, Gierens A, Hollig H, Hellhammer DH. Stress-induced immunomodulation is altered in patients with atopic dermatitis. J Neuroimmunol. 2002 ; 129 : 161-7.   DOI
9 Cooper KD. Atopy dermatitis-Recent trends in pathogenesis and therapy. J Invest dermatol. 1994 ; 102 : 128-65.   DOI
10 Mosman TR, Cherwinski HM, Bond MW, Giedlin MA, Coffman RL. Two types of murine helper T cell clones. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986 ; 136 : 2348-57.
11 Lee HR. Atopic Dermatitis. Korean J Pediatr 2000; 43 :1161-1167.
12 Cambazard F. The use of topical corticosteroids in atopic dermatitis in children. Ann Dermatol Venereol. 2005 ; 132(1) : 64-7.
13 Hajar T, Leshem YA, Hanifin JM, Nedorost ST, Lio PA, Paller AS, Block J, Simpson EL. A systematic review of topical corticosteroid withdrawal ("steroid addiction") in patients with atopic dermatitis and other dermatoses. J Am Acad Dermatol. 2014 ; 72(3) : 541-9.   DOI
14 Hong WS. Huangdi Neijing Suwen. Seoul : Research Institute of Oriental Medicine Publishing. 1985 : 72-80, 246-8.
15 Park SN. Effect of Sopungsan in BALB/c Mouse Model of Atopic Dermatitis. Doctoral Dissertation. Kyunghee University. 2004.
16 Cha KB, Kim YS, Seol IC. Literatual study on Atopic dermatitis. J Oriental Med Daejeon Univ. 2005 ; 14(2) : 113-26.
17 The National College of Oriental Medicine Herbology Classroom. Herbology. Seoul : Youngrimsa. 2016 : 557-8.
18 Kang SY, Han JH, Kim KY. Effect of Fructus Tribuli extract on regional cerebral blood flow and pial arterial diameter. Kor J Herbol. 1998 ; 8(1) : 187-200.
19 Yoo YJ, Lee HS. Effects of Fructus Tribuli on the Blood Pressure in Spontaneously Hypertensive Rats. J Trad Korean Med. 1999 ; 9(1) : 192-9.
20 Hong S. Effect of Fructus Tribuli extract on blood pressure and artery. Kor J Orient Int Med. 1997 ; 18(2) : 151-9.
21 Wei NI, Zhou G, HU LL, Tian DL, Jiang XX, OUYANG CH. Experimental study on the anti-inflammatory, anti-anoxia and anti-fatigue effects of extract from Tribulus terrestris L. Lishiznen Med Mater Med Res. 2007 ; 18(11) : 2778-9.
22 Lee DH, Yu HH, Jung SY, Moon HD, Kim SM, Jeon BH, You YO. Anticariogenic Properties of the Ethanol Extract of Tribuli fructus against Streptococcus mutans. Kor J Oriental Physiology & Pathology. 2007 ; 21(5) : 1148-53.
23 Chou CY. Kim YB. The Effects of Tribulus terrestris on Model of Allergic Rhinitis. Kor J Oriental Medical Ophthalmol Otolaryngol Dermatol. 2011 ; 24(2) : 28-34.
24 Zhang JD, Cao YB, Zheng XU. In Vitro and in Vivo Antifungal Activities of the Eight Steroid Saponins from Tribulus terrestris L. with Potent Activity against Fluconazole-Resistant Fungal. Biol Pharm Bull. 2005 ; 28(12) : 2211-5.   DOI
25 Xiao PG. Modern Chinese materia medica. Beijing : China Social Sciences Press. 2001.
26 Kim SA, Kang YH. Changes in Ceramide in Stratum Corneum and Anti-inflamatory Effects of Sopungdojeok-tang on Atopic Dermatitis. Korean J Orient Int Med. 2006 ; 27(1) : 72-83.
27 Yang HJ, Park KW, Kim HS, Cho SM, Park KM. Effect of Anti-atopic Allergic Reaction in Response to Oriental Herb Extracts. Kor J Food Sci Technol. 2010 ; 42(1) : 109-14.
28 Park YL, Park J. Pathogenesis of Atopic Dermatitis. Kor J of Investigative Dermatology. 2007 ; 14(3) : 67-72.
29 Poole JA, Rosenwasser LJ. The role of immunoglobulin E and immune inflammation: implications in allergic rhinitis. Curr Allergy Asthma Rep. 2005 ; 5(3) : 252-8.   DOI
30 Metcalfe DD, Baram D, Mekori YA. Mast cells. Physiol Rev. 1997 ; 77(4) : 1033-79.   DOI
31 Shirakawa I, Deichmann KA, Izuhara I, Mao I, Adra CN, Hopkin JM. Atopy and asthma: genetic variants of IL-4 and IL-13 signalling. Immunol Today. 2000 ; 21(2) ; 60-4.   DOI
32 Ahn KM. Role of Mast Cells in Allergic Inflammation and Innate Immunity. Korean J Pediatr. 2004 ; 47(11) : 1137-41.
33 Egle P, Neil F. The RBL-2H3 cell line: its provenance and suitability as a model for the mast cell. Inflamm Res. 2009 ; 58(11) : 737-45.   DOI
34 Chrousos GP. Stress, chronic inflammation, and emotional and physical well-being : Concurrent effects and chronic sequelae. J Allergy Clin Immunol. 2000 ; 106(5) : 275-91.   DOI
35 Kim NU. Inhibitory mechanism of curcumin on activation of mast cells for anti-allergic activity. Master Dissertation. Konkuk University. 2007.
36 Gilfillan AM, Tkaczyk C. Integrated signaling pathways for mast cell activation. Nat Rev Immunol. 2006 ; 6 : 218-30.   DOI
37 Cobb MH, Goldsmith EJ. Dimerization in MAP-kinase signaling. Trends Biochem. Sci. 2000 ; 25 : 7-9.   DOI
38 Theoharidies TC, Kalogeromitros D. The critical role of mast cells in allergy and inflammation. Ann N Y Acad Sci. 2006 ; 1088 : 78-99.   DOI
39 Gary LJ, Razvan L. Mitogen-Activated Protein Kinase Pathways Mediated by ERK, JNK, and p38 Protein Kinases. Science. 2002 ; 298(5600) : 1911-2.   DOI
40 Andrea H, Thomas K, Josef P, Konrad S. Physiology and pathophysiology of phingolipid metabolism and signaling. Biochem Biophys Acta. 2000 ; 1485 : 63-99.
41 Baeuerle PA, Baltimore D. NF-kappa B: ten years after. Cell. 1996 ; 87 : 13-20.   DOI
42 Kim SA, Kang YH. Changes in Ceramide in Stratum Corneum and Anti-inflamatory Effects of Sopungdojeok-tang on Atopic Dermatitis. Kor J Orient Int Med. 2006 ; 27(1) : 72-83.