Journal of the Korean Society of Manufacturing Technology Engineers (한국생산제조학회지)

The Korean Society of Manufacturing Technology Engineers (한국생산제조학회)
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Evaluation of Mechanical Tearing based Cell Disruption Capability to Shape Nanostructures formed on Nanoporous Alumina Filter

다공성 알루미나 필터 표면에 형성된 나노구조물의 형상에 따른 찢어짐에 의한 세포파쇄 특성 평가

  • Lee, Yong-Hun (Department of Advanced Mechanical Engineering, Kangwon National University) ;
  • Han, Eui-Don (Department of Advanced Mechanical Engineering, Kangwon National University) ;
  • Kim, Byeong-Hee (Department of Advanced Mechanical Engineering, Kangwon National University) ;
  • Seo, Young-Ho (Department of Advanced Mechanical Engineering, Kangwon National University)
  • Received : 2016.10.11
  • Accepted : 2017.01.10
  • Published : 2017.02.15
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Abstract

This study investigated the mechanical tearing of a cell membrane using a nanostructured alumina filter for easy and quick mechanical cell disruption. Nanostructured alumina filters were prepared by a multi-step aluminum anodizing process and nanopore etching process. Six different types of nanostructures were formed on the surface of the nanoporous alumina filters to compare the mechanical cell disruption characteristics according to the shape of the nanostructure. The prepared alumina filter was assembled in a commercial filter holder, and then, NIH3T3 fibroblast cells in a buffer solution were passed through the nanostructured alumina filter at a constant pressure. By measuring the concentration of proteins and DNA, the characteristics of mechanical cell disruption of the nanostructured alumina filter were investigated.

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References

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