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http://dx.doi.org/10.7735/ksmte.2017.26.1.1

Evaluation of Mechanical Tearing based Cell Disruption Capability to Shape Nanostructures formed on Nanoporous Alumina Filter  

Lee, Yong-Hun (Department of Advanced Mechanical Engineering, Kangwon National University)
Han, Eui-Don (Department of Advanced Mechanical Engineering, Kangwon National University)
Kim, Byeong-Hee (Department of Advanced Mechanical Engineering, Kangwon National University)
Seo, Young-Ho (Department of Advanced Mechanical Engineering, Kangwon National University)
Publication Information
Journal of the Korean Society of Manufacturing Technology Engineers / v.26, no.1, 2017 , pp. 1-5 More about this Journal
Abstract
This study investigated the mechanical tearing of a cell membrane using a nanostructured alumina filter for easy and quick mechanical cell disruption. Nanostructured alumina filters were prepared by a multi-step aluminum anodizing process and nanopore etching process. Six different types of nanostructures were formed on the surface of the nanoporous alumina filters to compare the mechanical cell disruption characteristics according to the shape of the nanostructure. The prepared alumina filter was assembled in a commercial filter holder, and then, NIH3T3 fibroblast cells in a buffer solution were passed through the nanostructured alumina filter at a constant pressure. By measuring the concentration of proteins and DNA, the characteristics of mechanical cell disruption of the nanostructured alumina filter were investigated.
Keywords
Nano-scale spikes; Mechanical cell disruption; Aluminum anodizing; Alumina filter;
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Times Cited By KSCI : 2  (Citation Analysis)
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