Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Optimization of in vitro lily culture system with different treatments of taurine

타우린 처리에 의한 나리 기내 식물체 생산체계 최적화

  • Lee, Sang-Hee (Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus. Konkuk university) ;
  • Yang, Hwan-Rae (Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus. Konkuk university) ;
  • Kim, Sun Tae (Department of Plant Bioscience, Pusan National University) ;
  • Jun, Tae Hwan (Department of Plant Bioscience, Pusan National University) ;
  • Kim, Yong Chul (Department of Plant Bioscience, Pusan National University) ;
  • Kim, Jong Bo (Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus. Konkuk university)
  • 이상희 (건국대학교 의료생명대학 생명공학과) ;
  • 양환래 (건국대학교 의료생명대학 생명공학과) ;
  • 김선태 (부산대학교 생명자원환경과학대학 식물생명과학과) ;
  • 전태환 (부산대학교 생명자원환경과학대학 식물생명과학과) ;
  • 김용철 (부산대학교 생명자원환경과학대학 식물생명과학과) ;
  • 김종보 (건국대학교 의료생명대학 생명공학과)
  • Received : 2017.12.06
  • Accepted : 2017.12.19
  • Published : 2017.12.31
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Abstract

Lilies as cut flowers are one of the most popular ornamental plants in South Korea. It is necessary to develop lily cultivars with high qualities. Therefore, highly efficient propagation systems are needed following release of elite cultivars. In this study, we used taurine treatment to improve the growth conditions including shoot and bulb formation, fresh weight gain, and reduction of rooting and browning. We experimentally evaluated the effect of taurine as a growth stimulator, at concentrations of 0, 2.5, 5, 10, 15 and 20 mg/l. The results showed that 20 mg of taurine enhanced shoot formation by 85% and increased fresh weight 5.5-fold, which was higher than the approximately four-fold increase in the control. In addition, multiple bulb formation rate was increased by 80% and rooting by 82% following exposure to 20 mg/l of taurine. The efficiency of taurine treatment was higher than that of control with 50% multiple bulb formation rate and 60% rooting rate. The browning was 10.6% at 2.5 mg/l of taurine when compared with 0.8% at 20 mg/l. Taurine showed a positive effect on the overall growth of lily plants in terms of increased fresh weight, shoot formation rate, rooting, and formation of multiple bulbs, indicating that taurine can be used as an alternative to amino acids or as an antioxidant such as citrate and vitamin C in plant tissue culture.

나리는 절화용으로 대한민국에서 인기 있는 작물 중 하나로 고품질의 우수한 나리 품종이 개발되어야 한다. 이러한 우수 품종이 개발된 후에는 고효율의 번식체계가 확립되어야 한다. 본 연구는 타우린 처리에 의해서 신초 및 구근형성, 생체중 증가 및 발근율 향상 등의 생장촉진 효과와 갈변율 감소효과를 통한 나리 '레드플레임' 품종의 기내 식물체 생육 조건 체계 확립을 위해 수행되었다. 타우린이 생장촉진물질로 효능이 있는지 검증하기 위해 타우린을 0, 2.5, 5. 10, 15 및 20 mg/l을 첨가하였다. 그 결과, 타우린 20 mg/l 처리구에서 85 %의 신초형성률을 나타내었고 생체중은 5.5배 증가로 무처리구에서의 4.35배보다 더 높은 증가를 보였다. 구근형성 및 발근율 촉진 효과로는 타우린 20 mg/l에서 80%의 multiple 구근 형성률과 82%의 발근율을 나타내었다. 무처리구는 각각 50%의 multiple 구근 형성률과 60%의 발근율로 타우린을 첨가한 처리구의 효율이 더높았다. 또한 타우린 2.5 mg/l 처리구에서는 10.6%의 갈변율을 보인 반면에, 20 mg/l 처리구에서 0.8%의 갈변율을 보여 갈변율 감소에도 효과를 나타내었다. 타우린 처리구가 생체중 증가, 신초 생성률, 발근율 및 구근 형성 등의 전반적인 생장요인 들에 있어서 긍정적인 효과를 보여 줌으로써 나리를 포함하는 식물조직배양에서 타우린이 구연산 및 비타민 C와 같은 항산화제 또는 아미노산 대체제로서 사용 가능성을 보여 주었다.

Keywords

References

  1. Anthony JM, Seneratna T, Dixon KW, Sivaisithamparam K (2004) The role of antioxidants for initiation of somatic embryos with Conostephium pendulum (Ericaceae). Plant Cell Tiss organ Cult 78(3):247-252 https://doi.org/10.1023/B:TICU.0000025661.56250.b4
  2. Arnaldos TL, Munoz R, Ferrer MA, Calderon AA (2001) Changes in phenol content during strawberry (Fragariaⅹananasa, cv. Chandler) callus culture. Physiol Plant 113:315-322 https://doi.org/10.1034/j.1399-3054.2001.1130303.x
  3. Druart PH (1988) Regulation of auxiliary branching in micropropagation of woody fruit species. Acta Hort 227:369-380
  4. Ferreira BAL, Lima-Costa ME (2006) Metabolic response to salt stress in cell suspension cultures of sensitive and resistant citrus. J Hortic Sci Biotechnol 81(6):983-987 https://doi.org/10.1080/14620316.2006.11512186
  5. George EF, Hall MA, De Klerk GJ (2008) Plant propagation by tissue culture. The background. 3rd ed. Dordrecht: Springer. ISBN 978-1-4020-5004-6
  6. Govaerts R (2013) World Checklists of Liliaceae. Royal Botanic Gardens, Kew. http//appa.kew.org/wcsp/qsearch.do.
  7. Han DS, Niimi Y, Nakano M (1997) Regeneration of haploid plants from anther cultures of the Asiatic hybrid lily 'Connecticut King'. Plant Cell Tiss Organ Cult 47:153-158 https://doi.org/10.1007/BF02318951
  8. Hao LH, He PQ, Liu CY, Chen KS, Li GY (2004) Physiological effects of taurine on the growth of wheat (Triticum aestivum L.) seedlings Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Bao. Chin J Plant Physiol Mol Biol 30(5):595-598
  9. Jeong JH (1998) Effect of scale position, chilling duration and IBA on bulblet formation in scale cuttings of Lilium hansonii. Flower Res J 23:33-41
  10. Joshi SK, Dhar U (2009) In vitro propagation from axenic explants of Lilium oxypetalum (D. Don) Baker, an endemic bulbous plant of high altitude Himalaya. Acta Physiol Plant 31:833-838 https://doi.org/10.1007/s11738-009-0299-y
  11. Kim JB (2017) Optimization of a protocol for the production of transgenic lily plants via particle bombardment. J Plant Biotechnol 44:82-88 https://doi.org/10.5010/JPB.2017.44.1.082
  12. Ko WH, Su CC, Chen CL, Chao CP (2009) Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid. Plant Tiss Organ Cult 96(2):137-141 https://doi.org/10.1007/s11240-008-9469-7
  13. Lee DH (2015) In vitro analysis of taurine as anti-stress agent in tomato (Solanum Lycopersicum)-preliminary study, Taurine 9, pp75-85, Springer Switzerland
  14. Liu J, Zhang J, Xu B, Jia C, Zhang J, Tan G, Jin Z (2011) Regeneration and production of transgenic Lilium longiflorum via Agrobacterium tumefaciens. In Vitro Cell Dev Biol-Plant 47:348-356 https://doi.org/10.1007/s11627-010-9336-1
  15. Marinangeli PA, Curvetto N (1997) Bulb quality and traumatic acid influence bulblet formation from scaling propagation of Lilium longiflorun. J Amer Soc Hort Sci 128:324-329
  16. Ministry of Food, Agriculture, Forestry and Fisheries (2015) Statistics of floriculture cultivation in 2015
  17. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Planta 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  18. Ozygit II (2008) Phenolic changes during in vitro organogenesis of cotton (Gossypium hirsutum L.) shoot tips. Afr J Biotech 7(8): 1145-1150
  19. Paek KY (2001) Plant Tissue Culture, Hyang-Moon publisher, Seoul, Korea, p466
  20. Roubelakis-Angelakis KA, Zivanivitc SB (1991) A new culture medium for in vitro rhizogenesis of grapevine (Vitis spp.) genotypes. HortSciences 26(12):1551-1553
  21. Roh HS, Lee SI, Kang YI, Kim MS, Kim JB (2013) Effects of ascorbic acid, citric acid and silver nitrate on the growth of in vitro lily plantlets and reduction of browning. J Plant Biotechnol 40: 224-230 https://doi.org/10.5010/JPB.2013.40.4.224
  22. Tang YP, Liu XQ, Gitutu RW, Chen LQ (2010) Callus induction and regeneration from in vitro cultured leaves, petioles and scales of Lilium leucanthum (Baker) Baker. Biotechnol Biotechnol Equip 4:2071-2076
  23. Wang Y, van Kronenburg B, Menzel T, Maliepaard C, Shen X, Krens F (2012) regeneration and Agrobacterium-mediated transformation of multiple lily cultivars. Plant Cell Tiss Organ Cult 111:113-122 https://doi.org/10.1007/s11240-012-0172-3
  24. Woo JH, Nam HH, Lee HS, Choi BK, Park IS, Kim KW (2004) Elimination of lily symptomless virus through shoot apex culture in Lilium oriental hybrids. Kor J Hor Sci Technol 22:328-332
  25. Yoo YK, Kim BW (2006) Effect of scale position and cutting condition on bulblet formation and Growth in scaling of Lilium oriental hybrid 'Siberia' by use of perforated polyethylene film bag. J Kor Soc People Plants Environ 9(1):40-45
  26. Zoltan M, Virag E, Ordog V (2011) Natural substances in tissue culture media of higher plants. Acta Biol Szeg 55(1):123-127