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http://dx.doi.org/10.5010/JPB.2017.44.4.484

Optimization of in vitro lily culture system with different treatments of taurine  

Lee, Sang-Hee (Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus. Konkuk university)
Yang, Hwan-Rae (Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus. Konkuk university)
Kim, Sun Tae (Department of Plant Bioscience, Pusan National University)
Jun, Tae Hwan (Department of Plant Bioscience, Pusan National University)
Kim, Yong Chul (Department of Plant Bioscience, Pusan National University)
Kim, Jong Bo (Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus. Konkuk university)
Publication Information
Journal of Plant Biotechnology / v.44, no.4, 2017 , pp. 484-489 More about this Journal
Abstract
Lilies as cut flowers are one of the most popular ornamental plants in South Korea. It is necessary to develop lily cultivars with high qualities. Therefore, highly efficient propagation systems are needed following release of elite cultivars. In this study, we used taurine treatment to improve the growth conditions including shoot and bulb formation, fresh weight gain, and reduction of rooting and browning. We experimentally evaluated the effect of taurine as a growth stimulator, at concentrations of 0, 2.5, 5, 10, 15 and 20 mg/l. The results showed that 20 mg of taurine enhanced shoot formation by 85% and increased fresh weight 5.5-fold, which was higher than the approximately four-fold increase in the control. In addition, multiple bulb formation rate was increased by 80% and rooting by 82% following exposure to 20 mg/l of taurine. The efficiency of taurine treatment was higher than that of control with 50% multiple bulb formation rate and 60% rooting rate. The browning was 10.6% at 2.5 mg/l of taurine when compared with 0.8% at 20 mg/l. Taurine showed a positive effect on the overall growth of lily plants in terms of increased fresh weight, shoot formation rate, rooting, and formation of multiple bulbs, indicating that taurine can be used as an alternative to amino acids or as an antioxidant such as citrate and vitamin C in plant tissue culture.
Keywords
Browning; lily; mass propagation; regeneration; taurine;
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