Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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The protective efficacy of the enterotoxigenic Escherichia coli vaccine candidate by GI24 against neonatal piglet colibacillosis

  • Choi, Yeong Hwan (College of Veterinary Medicine, Chonbuk National University) ;
  • Moon, Ja Young (College of Veterinary Medicine, Chonbuk National University) ;
  • Seo, Byoung Joo (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Won Kyung (College of Veterinary Medicine, Chonbuk National University) ;
  • Cho, Jeong Sang (College of Veterinary Medicine, Chonbuk National University) ;
  • Choi, Min Su (College of Veterinary Medicine, Chonbuk National University) ;
  • Lim, Jae Sam (Chungcheongnam-Do Livestock Technology Research Institute) ;
  • Kim, Sung Bok (Chungcheongnam-Do Livestock Technology Research Institute) ;
  • Kim, Won Il (College of Veterinary Medicine, Chonbuk National University) ;
  • Hur, Jin (College of Veterinary Medicine, Chonbuk National University)
  • Received : 2017.11.12
  • Accepted : 2017.12.06
  • Published : 2017.12.30
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Abstract

Enterotoxigenic Escherichia coli (ETEC) strains producing each F4, F5, F6 and F41 fimbriae were lysed by GI24 peptide. The lysate cells were used as ETEC vaccine candidate. This study was carried out to examine whether intramuscular (im) immunization of pregnant sows with the novel vaccine candidate could effectively protect their neonatal piglets against ETEC colibacillosis. All pregnant sows were primed at 11 weeks and were boosted at 14 weeks of pregnancy. Group A sows were im inoculated with PBS. Group B sows were im immunized with $2{\times}10^9$ the mixture. Seral IgG, colostral IgA and IgG titers from group B sows, and seral IgG and IgA levels in group B piglets were significantly higher than those of group A sows and piglets, respectively. After challenge with wild-type ETEC, diarrhea and mortality was not observed in group B piglets. However, diarrhea was observed in 66.7% of group A piglets, and 33.3% mortality was observed. These findings indicate that im immunization of sows with the mixture of the novel vaccine candidate can effectively protect their offspring from ETEC colibacillosis.

Keywords

References

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