Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effect of ginger and cinnamon extract mixtures on the growth of intestinal bacteria and intestinal inflammation

생강계피 복합물이 장내 유익균 증식 및 염증조절 기능에 미치는 영향

  • Kim, Min Ju (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Kim, Min Seo (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Kang, Sung Tae (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Kim, Ji Yeon (Department of Food Science and Technology, Seoul National University of Science and Technology)
  • Received : 2017.08.16
  • Accepted : 2017.09.13
  • Published : 2017.12.01
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Abstract

We aimed to assess the potential growth-promoting effects of ginger and cinnamon mixtures (GCM) on intestinal bacteria and their anti-inflammatory effects in a cellular model of intestinal inflammation. Bifidobacterium longum, Lactobacillus sp., and Lactobacillus acidophilus served as intestinal bacteria. Further, in the inflammatory co-culture model, Caco-2 cells co-cultured with RAW264.7 cells were treated with GCM before the addition of lipopolysaccharide (LPS) to induce inflammation in RAW264.7 cells. Addition of GCM to modified Eggerth Gagnon media at a ginger:cinnamon ratio of 1:5 increased the growth of B. longum, Lactobacillus sp., and L. acidophilus compared to that of the control. In a cellular model, compared to LPS-treated groups, GCM-treated groups maintained high transepithelial electrical resistance at ginger:cinnamon ratios of 1:1, 1:3, 1:5, and 1:7 and decreased the tight junction permeability at 3:1, 1:1, 1:3, and 1:5 ratios, similar to that shown by the control groups. In addition, GCM-treated groups showed decreased levels of nitrite at 1:1, 1:5, and 1:7 ginger:cinnamon ratios. Based on these results, it can be concluded that among the various combinations of GCM, the ginger:cinnamon ratio of 1:5 is the optimal composite ratio that shows positive effects on the intestinal beneficial bacteria and in anti-inflammation.

본 연구에서 생강과 계피 추출물 및 복합물의 장내 개선 효능을 조사하였다. 생강계피 복합물을 Modified EG 액체배지에 처리하고 대표적인 유익균인 B. longum, Lactobacillus sp.와 L. acidophilus 균주를 접종하여 생육 활성을 관찰하였다. 그리고 사람의 장상피세포인 Caco-2 cell과 마우스의 대식세포인 RAW 264.7을 co-culture한 model에서 생강계피 복합물을 처리한 후 lipopolysaccharide (LPS)로 자극시켜 Transepithelial electrical resistance (TEER), permeability 그리고 nitrite 생성량을 확인하였다. 그 결과, 생강계피 복합물을 생강 : 계피 1:5로 처리 시, B. longum, Lactobacillus sp. 및 L. acidophilus 세 가지 균주의 성장을 모두 최대로 상승시켜 생육 활성 효과가 우수하였다. 특히 생강계피 복합물은 대조군에 비해 Lactobacillus sp. 균주의 가장 높은 생육 상승을 나타내어 생강계피 복합물에 젖산균의 생육인자를 함유할 것으로 추정된다. 반면 장 염증활성 조절을 확인하기 위한 co-culture model에서는, LPS 처리군과 비교하여, 생강계피 복합물 처리군 중 생강 : 계피 1 : 1, 1 : 3, 1 : 5와 1 : 7 복합물이 대조군과 비슷하거나 더 높게 증가하였고, tight junction(TJ) permeability는 생강 : 계피 3 : 1, 1 : 1, 1 : 3과 1 : 5 복합물에서 대조군과 비슷하게 감소하였다. 또한, 생강: 계피 1 : 1, 1 : 5와 1 : 7 복합물은 nitrite의 수준을 감소시켰다. 장내 유익균의 생육 및 장 염증 억제 활성 결과를 종합하여 볼 때 생강계피 복합물 중 생강 : 계피 1 : 5 복합물이 장내 유익균과 염증 조절에 좋은 효과를 나타내는 최적 복합비율이라고 판단할 수 있다.

Keywords

References

  1. Ahn YJ, Kim MJ, Kawamura T, Yamamoto T, Fujisawa T, Mitsuoka T (1990a) Effect of Panax ginseng extract on growth responses of human intestinal bacteria and bacterial metabolism. Korean J Ginseng Sci 14: 253-264
  2. Ahn YJ, Sakanaka S, Kim MJ, Kawamura T, Fujisawa T, Mitsuoka T (1990b) Effect of green tea extract on growth of intestinal bacteria. Microb Ecol Health Disease 3: 335-338 https://doi.org/10.3109/08910609009140256
  3. Aktan F (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci 75: 639-653 https://doi.org/10.1016/j.lfs.2003.10.042
  4. Cho YJ (2011) Anti-inflammatory Effect of Jatrorrhizine from Phellodendron amurense in Lipopolysaccharide-stimulated Raw264.7 Cells. J Appl Biol Chem 54(2): 114-119 https://doi.org/10.3839/jabc.2011.020
  5. Fuller R (1992) Probiotics. The scientific basis. Chapman and Hall, London
  6. Han EJ (2012) Quality characteristics of muffins containing ginger juice. Korean J Culinary Research, 18(5): 256-266
  7. Jeong ET, Park MY, Lee JG, Chang DS (1998) Antimicrobial activity and antimutagenesis of cinnamon (Cinnamomum cassia Blume) bark extract. J Fd Hyg Safety 13: 337-343
  8. Jeong HM, KIm YS, Ahn SJ, Auh MS, Ahn JB, Kim KY (2011) Effect of Zizyphus jujuba var. boeunesis extracts on the growth of intestinal microflora and its antioxidant activities. J Korean Soc Food Sci Nutr 40(4): 500-508 https://doi.org/10.3746/jkfn.2011.40.4.500
  9. Ji MS, Park MJ, Lee MY, Kim JG, Ko BS (2006) Effect of Hehotang extrac on the growth of intestinal bacteria and immunostimulation. Korean J. Food Sci technol 38: 104-108
  10. Jung JW, Ko JH, Ko WM, Park JH, Baek YS (2017) Isoprenylated flavonoids from the root bark of Morus alba L. and their inhibition effect on NO production in LPS-induced RAW 264.7 cells. J Appl Biol Chem 60(2): 109-111 https://doi.org/10.3839/jabc.2017.018
  11. Kang SY, Kim TG, Park MS, Han HM, Jung KK, Kang JH, Moon A, Kim SH (1999) Inhibitory effects of Eugenia caryophyllate, Ephedra sinica and Cinnamomum cassia on the replication of HBV in HepG2 2.2.15 cells. J Appl Pharmacology 7: 133-137
  12. Kim CY (2016) Inhibition of Interleukin-1a-induced Intestinal Epithelial Tight Junction Permeability by Curcumin Treatment in Caco-2 Cells. J Life Sci 26: 1082-1087 https://doi.org/10.5352/JLS.2016.26.9.1082
  13. Kim EJ, Ahn MS (1993) Antioxidative effect of ginger extracts. Korean J Soc Food Sci 9: 37-42
  14. Kim JY, Kim DM (2016) Nutritional composition containing ginger extract for enhancing tight junctions in intestinal cells and prepeparing method thereof. Korea Patent 10-2016-009166, 19 Jul 2016
  15. Kim KM, Kim YS, Lim JY, Min SJ, Ko HC, Kim SJ, Kim Y (2015) Intestinal anti-inflammatory activity of Sasa quelpaertensis leaf extract by suppressing lipopolysaccharide-stimulated inflammatory mediators in intestinal epithelial Caco-2 cells co-cultured with RAW 264.7 macrophage cells. Nutr Res Pract 9: 3-10 https://doi.org/10.4162/nrp.2015.9.1.3
  16. Kim L, Kwon O, Kim JY (2015) Comparison of Protective Effects of Young and Ripened Persimmon Extracts against Inflammatory Stress Induced by Deoxycholic Acid in Small Intestinal Cells. J Korean Soc Food Sci Nutr 44(10): 1583-1587 https://doi.org/10.3746/jkfn.2015.44.10.1583
  17. Kim ML, Choi KH, Park CS (2000) Growth inhibition of food-borne bacteria by juice and extract of Ginger and Garlic. J, East Asian Soc. Dietary Life 10: 160-169
  18. Kim Y, Kim DM, Kim JY (2017) Ginger Extract Suppresses Inflammatory Response and Maintains Barrier Function in Human Colonic Epithelial Caco-2 Cells Exposed to Inflammatory Mediators. J Food Sci 82: 1264-1270 https://doi.org/10.1111/1750-3841.13695
  19. Lee EH, Cho JH, Kim DH, Hong SH, Kim NH, Park MJ, Hong EJ, Cho YJ (2017) Anti-inflammatory activity of manassantin A from ultra-fine ground Saururus chinensis in lipopolysaccharide-stimulated RAW 264.7 cells. Appl Biol Chem 60(1): 63-71 https://doi.org/10.1007/s13765-016-0249-5
  20. Lee HS (2009) Chemical Composition of Cinnamomum cassia Leaf Oils and Suppression Effect of Cinnamyl Alcohol on Nitric Oxide Synthase. Appl Biol Chem 52: 480-485
  21. Lee J, Ko S, Kim H, Kwon H (2011) Integrity and cell-monolayer permeability of chitosan nanoparticles in simulated gastrointestinal fluids. Food Sci Biotechnol 20(4): 1033-1042 https://doi.org/10.1007/s10068-011-0141-3
  22. Lee MY, Kim H, Shin KS (2015) In vitro and in vivo effects of polysaccharides isolated from Korean persimmon vinegar on intestinal immunity. J Korean Soc Appl Biol Chem 58(6): 867-876 https://doi.org/10.1007/s13765-015-0117-8
  23. Lee SH, Shin HK (1995) Effect of the fractionated extracts of mugwort on the in vitro growth of some intestinal microorganism. Korean J. Nutr 28: 1065-1072
  24. Li Rui, Chen Wei, Li Xu (2008) A novel anticancer effect of butein: Inhibition of invasion through the ERK1/2 and NF-${\kappa}B$ signaling pathways in bladder cancer cells. FEBS Letters 582, doi: 10.1016/j.febslet.2008.04.046
  25. Liao JC, Deng JS, Chiu CS, Hou WC, Huang SS, Shie PH, Huang GJ (2012) Anti-Inflammatory Activities of Cinnamomum cassia Constituents In Vitro and In Vivo. Evid Based Complement Alternat Med 2012:429320
  26. Mitsuoka T (1980) A color atlas of anaerobic bacteria. Sobunsha, Tokyo
  27. Olejnik A, Kowalska K, Kidon M, Czapski J, Rychlik J, Olkowicz M, Dembczynski R (2016) Purple carrot anthocyanins suppress lipopolysaccharide-induced inflammation in the co-culture of intestinal Caco-2 and macrophage RAW264.7 cells. Food Funct 7: 557-564 https://doi.org/10.1039/C5FO00890E
  28. Pandey MK, Sung B, Ahn KS, Aggarwal BB (2009) Butein Suppresses Constitutive and Inducible Signal Transducer and Activator of Transcription (STAT) 3 Activation and STAT3-Regulated Gene Products through the Induction of a Protein Tyrosine Phosphatase SHP-1. Molecular Pharmacology 75(3): 525-533 https://doi.org/10.1124/mol.108.052548
  29. Park KH, Koh DS, Lim YH (2001) Anti-allergic compound isolated from Cinnamomum cassia. J Korean Soc Agric Chem Biothechnol 44: 40-42
  30. Park KR, Lee WJ, Cho MG, Park ES, Jeong JY, Kwon OS, Yoon HS, Kim KY (2010) Effects of the extract from Gyrophora esculenta and Coriolus versicolor judae mycelia on the growth of intestinal bactera. J Korean Soc Food Sci Nutr 39(6): 820-825 https://doi.org/10.3746/jkfn.2010.39.6.820
  31. Park RJ, Park YK (2000) Studies on the effects of the parts of Cinamomum cassia Presl on the antioxidation (I). Kor J Herbology 15: 45-55
  32. Romier-Crouzet B, Van De Walle J, During A, Joly A, Rousseau C, Henry O, Larondelle Y, Schneider YJ (2009) Inhibition of inflammatory mediators by polyphenolic plant extracts in human intestinal Caco-2 cells. Food Chem Toxicol 47: 1221-1230 https://doi.org/10.1016/j.fct.2009.02.015
  33. Sung KC (2010) A study on the pharmaceutical characteristics and analysis of natural ginger extract. J of the Korean Oil Chemists' Soc, 27: 266-272
  34. Suzuki T, Hara H (2011) Role of flavonoids in intestinal tight junction regulation. J Nutr Biochem 22: 401-408 https://doi.org/10.1016/j.jnutbio.2010.08.001
  35. Ulluwishewa D, Anderson RC, McNabb WC, Moughan PJ, Wells JM, Roy NC (2011) Regulation of tight junction permeability by intestinal bacteria and dietary components. J Nutr 141: 769-776 https://doi.org/10.3945/jn.110.135657
  36. Wullaert A, Bonnet MC, Pasparakis M (2011) NF-eB in the regulation of epithelial homeostasis and inflammation. Cell Res 21: 146-158 https://doi.org/10.1038/cr.2010.175
  37. Wu SJ, Don TM, Lin CW, Mi FL (2014) Delivery of berberine using chitosan/fucoidan-taurine conjugate nanoparticles for treatment of defective intestinal epithelial tight junction barrier. Mar Drugs 12: 5677-5697 https://doi.org/10.3390/md12115677
  38. Yang WK, Jung CS, Jung KW, Km JW, Lee EB (1992) Antigastritic and antiulcerative action of the extract of Zingiberis Rhizoma. J Pharm Soc Korea 36: 173-179

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