Mycobiology

The Korean Society of Mycology (한국균학회)
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Antifungical Activity of Autochthonous Bacillus subtilis Isolated from Prosopis juliflora against Phytopathogenic Fungi

  • Abdelmoteleb, Ali (Instituto de Ciencias Agricolas de la Universidad Autonoma de Baja California (ICA-UABC)) ;
  • Troncoso-Rojas, Rosalba (Centro de Investigacion en Alimentacion y Desarrollo, A.C., Direccion de Tecnologia de Alimentos de Origen Vegetal) ;
  • Gonzalez-Soto, Tania (Centro de Investigacion en Alimentacion y Desarrollo, A.C., Direccion de Tecnologia de Alimentos de Origen Vegetal) ;
  • Gonzalez-Mendoza, Daniel (Instituto de Ciencias Agricolas de la Universidad Autonoma de Baja California (ICA-UABC))
  • Received : 2017.07.03
  • Accepted : 2017.10.26
  • Published : 2017.12.01
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Abstract

The ability of Bacillus subtilis, strain ALICA to produce three mycolytic enzymes (chitinase, ${\beta}$-1,3-glucanase, and protease), was carried out by the chemical standard methods. Bacillus subtilis ALICA was screened based on their antifungal activity in dual plate assay and cell-free culture filtrate (25%) against five different phytopathogenic fungi Alternaria alternata, Macrophomina sp., Colletotrichum gloeosporioides, Botrytis cinerea, and Sclerotium rolfesii. The B. subtilis ALICA detected positive for chitinase, ${\beta}$-1,3-glucanase and protease enzymes. Fungal growth inhibition by both strain ALICA and its cell-free culture filtrate ranged from 51.36% to 86.3% and 38.43% to 68.6%, respectively. Moreover, hyphal morphological changes like damage, broken, swelling, distortions abnormal morphology were observed. Genes expression of protease, ${\beta}$-1,3-glucanase, and lipopeptides (subtilosin and subtilisin) were confirmed their presence in the supernatant of strain ALICA. Our findings indicated that strain ALICA provided a broad spectrum of antifungal activities against various phytopathogenic fungi and may be a potential effective alternative to chemical fungicides.

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References

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