Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Botryosphaeria parva Causing Stem Blight on Rubus crataegifolius in Korea

  • Park, Sangkyu (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Seung-Han (Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Back, Chang-Gi (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Seung-Yeol (School of Applied Biosciences, Kyungpook National University) ;
  • Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University) ;
  • Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University)
  • Received : 2016.02.27
  • Accepted : 2016.05.31
  • Published : 2016.06.30
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Abstract

In 2015, stem blight of Rubus crataegifolius was observed in Pohang, Korea. The symptoms began as dark red spots in the stem, which led to stem blight, then leaf blight, and eventually resulted in death. A fungal isolate was obtained from a symptomatic stem and incubated on a potato dextrose agar plate. The isolated fungus produced white, cloudy mycelia turned black in 3 days. Based on the morphological characteristics, the causal fungus was assumed to be Botryosphaeria sp. A pathogenicity test was conducted according to Koch's postulates. To identify the causal agent, the combined sequence of the internal transcribed spacer, ${\beta}$-tubulin, and translation elongation factor $1{\alpha}$ genes were used for phylogenetic analysis. Approximately 1,200 bp of the combined sequence clearly suggested that the isolated pathogen was Botryosphaeria parva. This is the first report on stem blight in R. crataegifolius caused by B. parva in Korea.

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References

  1. Abdollahzadeh, J., Zare, R. and Phillips, A. J. 2013. Phylogeny and taxonomy of Botryosphaeria and Neofusicoccum species in Iran, with description of Botryosphaeria scharifii sp. nov. Mycologia 105: 210-220. https://doi.org/10.3852/12-107
  2. Carbone, I. and Kohn, L. M. 1999. A method for designing primer sets for the speciation studies in filamentous ascomycetes. Mycologia 91: 553-556. https://doi.org/10.2307/3761358
  3. Cheon, W., Kim, Y. S., Lee, S. G., Jeon, Y. H. and Chun, I. J. 2013. First report of branch dieback of walnut caused by Neofusicoccum parvum in Korea. Plant Dis. 97: 1114.
  4. Choi, I. Y., Sharma, P. K. and Cheong, S. S. 2012. First report of Neofusicoccum parvum associated with bark dieback of blueberry in Korea. Plant Pathol. J. 28: 217. https://doi.org/10.5423/PPJ.2012.28.2.217
  5. Glass, N. L. and Donaldson, G. C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61: 1323-1330.
  6. Haleem, R. A., Abdullah, S. K. and Jubrael, J. M. S. 2012. Identification and pathogenicity of Botryosphaeria parva associated with grapevine decline in Kurdistan region: Iraq. Acta Agrobot. 65: 71-78. https://doi.org/10.5586/aa.2012.045
  7. Korea Forest Service. 2015. 2014 Production of forest products. Korea Forest Service, Daejeon, Korea. p. 15. (In Korean)
  8. Kim, W. G., Koo, H. M., Kim, K. H., Hyun, I. H., Hong, S. K. and Cha, J. S. 2009. List of Plant Diseases in Korea. 5th ed. Korean Society of Plant Pathology, Suwon, Korea. pp. 500-501. (In Korean)
  9. Linaldeddu, B. T., Franceschini, A., Luque, J. and Philips, A. J. L. 2007. First report of canker disease caused by Botryosphaeria parva on cork oak trees in Italy. Plant Dis. 91: 324.
  10. Moon, K. M., Kim, J. E., Kim, H. Y., Lee, J. S., Son, G. A., Nam, S. W., Kim, B. W. and Lee, J. H. 2011. Antioxidant activity of Rubus crataegifolius Bge. fruit extracts. J. Life Sci. 21: 1214-1218. https://doi.org/10.5352/JLS.2011.21.9.1214
  11. Ni, W., Zhang, X., Bi, H., Iteku, J., Ji, L., Sun, C., Fang, J., Tai, G., Zhou, Y. and Zhao, J. 2009. Preparation of a glucan from the roots of Rubus crataegifolius Bge. and its immunological activity. Carbohydr. Res. 344: 2512-2528. https://doi.org/10.1016/j.carres.2009.08.042
  12. Page, R. D. 1996. TreeView: an application to display phylogenetic trees on personal computers. Comput. Appl. Biosci. 12: 357-358.
  13. Pennycook, S. R. and Samuels, G. J. 1985. Botryosphaeria and Fusicoccum species associated with ripe fruit rot of Actinidia deliciosa (kiwifruit) in New Zealand. Mycotaxon 24: 445-458.
  14. Phillips, A. J. L. 2002. Botryosphaeria species associated with diseases of grapevines in Portugal. Phytopathol. Mediterr. 41: 3-18.
  15. Sakalidis, M. L., Slippers, B., Wingfield, B. D., Hardy, G. E. J. and Burgess, T. I. 2013. The challenge of understanding the origin, pathways and extent of fungal invasions: global populations of the Neofusicoccum parvum-N. ribis species complex. Divers. Distrib. 19: 873-883. https://doi.org/10.1111/ddi.12030
  16. Slippers, B., Crous, P. W., Denman, S., Coutinho, T. A., Wingfield, B. D. and Wingfield, M. J. 2004. Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea. Mycologia 96: 83-101. https://doi.org/10.2307/3761991
  17. Slippers, B., Johnson, G. I., Crous, P. W., Coutinho, T. A., Wingfield, B. D. and Wingfield, M. J. 2005. Phylogenetic and morphological re-evaluation of the Botryosphaeria species causing diseases of Mangifera indica. Mycologia 97: 99-110. https://doi.org/10.3852/mycologia.97.1.99
  18. van Niekerk, J. M., Crous, P. W., Groenewald, J. Z., Fourie, P. H. and Halleen, F. 2004. DNA phylogeny, morphology and pathogenicity of Botryosphaeria species on grapevines. Mycologia 96: 781-798. https://doi.org/10.2307/3762112
  19. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, San Diego, CA, USA.
  20. Zhang, L., Li, J., Hogan, S., Chung, H., Welbaum, G. E. and Zhou, K. 2010. Inhibitory effect of raspberries on starch digestive enzyme and their antioxidant properties and phenolic composition. Food Chem. 119: 592-599. https://doi.org/10.1016/j.foodchem.2009.06.063