Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Optimal Extraction Conditions of Active Components from the Adventitious Roots of Noni (Morinda citrifolia)

노니 부정근으로부터 유효성분의 최적 추출조건

  • Kim, Myong-Ki (Department of Food Science and Engineering, Seowon University)
  • Received : 2016.01.28
  • Accepted : 2016.03.24
  • Published : 2016.04.30
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Abstract

To determine the optimum condition for the extraction of active components, noni adventitious roots were extracted under various conditions employing various solvents including methanolic water at different ratio (20, 40, 60, 80, and 100% water), extraction times and extraction methods. Anthraquinones, phenolic compounds, and flavonoids extraction using different solvents showed that 1 h of ultrasonic extraction was effective in 60-80% methanol, and 2 h of reflux extraction was effective in 80% methanol. To compare the extraction efficiency of active components according to different extraction methods and extraction times for noni adventitious roots, the active components were extracted by ultrasonic extraction, shaking extraction, reflux extraction, homogenizer extraction, high-pressure extraction, and soaking extraction. The highest phenolic contents were found in the extracted from ultrasonic extraction and anthraquinones and flavonoids contents were highest in the reflux extraction.

노니 부정근 유효성분의 효율적인 추출조건을 규명하고자 용매 종류, 물과 메탄올의 비율(물, 20, 40, 60, 80, 100%), 추출시간 및 추출방법을 달리하여 실험을 진행하였다. 안트라퀴논, 페놀성 화합물 및 플라보노이드의 용매별 추출한 결과 1시간 동안 초음파 추출 시 60-80% 메탄올에서 효과적이었으며, 환류냉각 추출기로 2시간 추출 시 80% 메탄올에서 효과적이었다. 노니 부정근의 유효성분에 대한 추출방법 및 시간에 따른 추출효율을 비교하기 위하여 교반추출, 환류냉각추출, 초음파추출, 균질기추출, 초고압추출, 침지추출을 실시하였다. 페놀성 화합물은 초음파추출에서 가장 높은 함량을 보였으며 안트라퀴논과 플라보노이드 함량은 환류냉각추출에서 가장 높게 나타났다.

Keywords

References

  1. Wang MY, West BJ, Jensen CJ, Nowicki D, Su C, Palu AK, Anderson G. Morinda citrifolia (noni): A literature review and recent advances in noni research. Acta Pharmacol. Sin. 23: 1127-1141 (2002)
  2. Yoo JS, Hwang JT, Yoo ES, Cheun BS. Study on herbal extract on the noni (Morinda citrifolia). Korean Soc. Biotechnol. Bioeng. J. 19: 110-112 (2004)
  3. Babu KS, Srinivas PV, Praveen B, Kishore KH, Murty US, Rao JM. Antimicrobial constituents from the rhizomes of Rheum emodi. Phytochemistry 62: 203-207 (2003) https://doi.org/10.1016/S0031-9422(02)00571-X
  4. Yang J, Paulino R, Janke-Stedronsky S, Abawi F. Free-radicalscavenging activity and total phenols of noni (Morinda citrifolia L.) juice and powder in processing and storage. Food Chem. 102: 302-308 (2007) https://doi.org/10.1016/j.foodchem.2006.05.020
  5. Hiramatsu T, Imoto M, Koyano T, Umezawa K. Induction of normal phenotypes in ras-transormed cells by damnacanthal from Morinda citrifolia. Cancer Lett. 73: 161-166 (1993) https://doi.org/10.1016/0304-3835(93)90259-C
  6. Kamiya K, Hamabe W, Tokuyama S, Hirano K, Satake T, Kumamoto-Yonezawa Y, Yoshida H, Mizushina Y. Inhibitory effect of anthraquinones isolated from the noni (Morinda citrifolia) root on animal A-, B- and Y-families of DNA polymerases and human cancer cell proliferation. Food Chem. 118: 725-730 (2010) https://doi.org/10.1016/j.foodchem.2009.05.053
  7. Zin ZM, Abdul-Hamid A, Osman A. Antioxidative activity of extracts from Mengkudu (Morinda citrifolia L.) root, fruit and leaf. Food Chem. 78: 227-231 (2002) https://doi.org/10.1016/S0308-8146(01)00402-2
  8. Choi BC, Sim SS. Anti-inflammatory activity and phospholipase $A_2$ inhibition of noni (Morinda citrifolia) methanol extracts. Yakhak Hoeji 49: 405-409 (2005)
  9. Wang MY, Su C. Cancer preventive effect of Morinda citrifolia (Noni). Ann. Ny. Acad. Sci. 952: 161-168 (2001) https://doi.org/10.1111/j.1749-6632.2001.tb02737.x
  10. Younos C, Rolland A, Fleurentin J, Lanhers MC, Misslin R, Mortier F. Analgesic and behavioural effects of Morinda citrifolia. Planta Med. 56: 430-434 (1990) https://doi.org/10.1055/s-2006-961004
  11. Muto J, Hosung L, Uwaya A, Isami F, Ohno M, Mikami T. Morinda citrifolia fruit reduces stress-induced impairment of cognitive function accompanied by vasculature improvement in mice. Physiol. Behav. 101: 211-217 (2010) https://doi.org/10.1016/j.physbeh.2010.04.014
  12. Zin ZM, Hamid AA, Osman A, Saari N. Antioxidative activities of chromatographic fractions obtained from root, fruit and leaf of Mengkudu (Morinda citrifolia L.). Food Chem. 94: 169-178 (2006) https://doi.org/10.1016/j.foodchem.2004.08.048
  13. Zenk MH, El-Shangi H, Achulte U. Anthraquinone production by Morinda citrifolia cell suspension culture. Planta Med. Suppl. l: 79-101 (1975)
  14. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555-559 (1999) https://doi.org/10.1016/S0308-8146(98)00102-2
  15. Folin O, Ciocalteu V. On tyrosine and tryptophane determinations in proteins. J. Biol. Chem. 73: 627-650 (1927)
  16. De Santis D, Moresi M. Production of alizarin extracts from Rubia tinctorum and assessment of their dyeing properties. Ind. Crop. Prod. 26: 151-162 (2007) https://doi.org/10.1016/j.indcrop.2007.02.002
  17. Wang, L, Li D, Bao C, You J, Wang Z, Shi Y, Zhang H. Ultrasonic extraction and separation of anthraquinones from Rheum palmatum L. Ultrason. Sonochem. 15: 738-746 (2008) https://doi.org/10.1016/j.ultsonch.2007.12.008
  18. Hemwimon S, Pavasant P, Shotipruk A. Microwave-assisted extraction of antioxidative anthraquinones from roots of Morinda citrifolia. Sep. Purif. Technol. 54: 44-50 (2007) https://doi.org/10.1016/j.seppur.2006.08.014
  19. Hemwimol S, Pavasant P, Shotipruk A. Ultrasound-assisted extraction of anthraquinones from roots of Morinda citrifolia. Ultrason. Sonochem. 13: 543-548 (2006) https://doi.org/10.1016/j.ultsonch.2005.09.009
  20. Jang HD, Chang KS, Chang TC, Hsu CL. Antioxidant potentials of buntan pumelo (Citrus grandis Osbeck) and its ethanolic and acetified fermentation products. Food Chem. 118: 554-558 (2010) https://doi.org/10.1016/j.foodchem.2009.05.020
  21. Pongnaravane B, Goto M, Sasaki M, Anekpankul T, Pavasant P, Shotipruk A. Extraction of anthraquinones from roots of Morinda citrifolia by pressurized hot water: Antioxidant activity of extracts. J. Supercrit. Fluid. 37: 390-396 (2006) https://doi.org/10.1016/j.supflu.2005.12.013
  22. Huang W, Xue A, Niu H, Jia Z, Wang J. Optimised ultrasonicassisted extraction of flavonoids from Folium eucommise and evaluation of antioxidant activity in multi-test systems in vitro. Food Chem. 114: 1147-1154 (2009) https://doi.org/10.1016/j.foodchem.2008.10.079