Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antioxidative Effects of Rhaphiolepis indica and Quercus salicina from Jeju

제주 자생 다정큼나무 및 참가시나무의 항산화 효과

  • Kim, Hye-Ran (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Park, Gyu-Nam (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Jung, Bo-Kyoung (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Yoon, Weon-Jong (Jeju Biodiversity Research Institute, Jeju Technopark) ;
  • Jung, Yong-Hwan (Jeju Biodiversity Research Institute, Jeju Technopark) ;
  • Chang, Kyung-Soo (Department of Clinical Laboratory Science, Catholic University of Pusan)
  • 김혜란 (부산가톨릭대학교 임상병리학과) ;
  • 박규남 (부산가톨릭대학교 임상병리학과) ;
  • 정보경 (부산가톨릭대학교 임상병리학과) ;
  • 윤원종 (제주테크노파크 생물종다양성연구소) ;
  • 정용환 (제주테크노파크 생물종다양성연구소) ;
  • 장경수 (부산가톨릭대학교 임상병리학과)
  • Received : 2016.02.04
  • Accepted : 2016.03.10
  • Published : 2016.03.30
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Abstract

In this study, the extracts from the leaves of Rhaphiolepis indica(R. indica) and Quercus salicina(Q. salicina) confirmed antioxidative effects. The antioxidative and cytotoxic effects of the two extracts were analyzed according to varied concentrations and time. The extracts of R. indica and Q. salicina showed dose-dependent DPPH radical scavenging activities. The extracts of R. indica and Q. salicina at concentrations of 5 mg/mL showed DPPH radical scavenging activities at 89.93 and 92.41%. Therefore, Q. salicina were confirmed to have higher antioxidative effects than R. indica. Total phenolic contents were 65.20 mg GAE/g for R. indica and 85.20 mg GAE/g for Q. salicina. The result that Q. salicina have higher total phenolic contents than R. indica suggested a correlation between total phenolic contents and DPPH radical scavenging activity. The cell protective effects of HepG2 and A549 cells under oxidative stress, both the extracts showed relatively low cell protective effects at around 10%. The Cytotoxic effects of A549 Cells did not show cytotoxicity at concentrations of $100{\mu}g/mL$ or below. The results of this study are likely to be used as basic data to develop antioxidants using the extract of R. indica and Q. salicina.

본 연구에서는 제주 천연 물질인 다정큼나무와 참가시나무 잎 추출물에서 항산화 효과를 확인하였다. 두 추출물에 대한 항산화 효과 및 세포독성 효과를 농도별, 시간별로 비교 분석한 바, 다정큼나무와 참가시나무 추출물에서 농도 의존적으로 DPPH 라디칼 소거능을 나타내었다. 다정큼나무와 참가시나무 추출물의 5 mg/mL 농도에서 89.93%, 92.41%의 DPPH 라디칼 소거능을 나타내어 다정큼나무보다 참가시나무 추출물에서 더 높은 항산화 효과를 확인하였다. 총 페놀 함량은 다정큼나무 추출물에서는 65.20 mg GAE/g, 참가시나무 추출물에서는 85.20 mg GAE/g 으로 참가시나무에서 더 높은 총 페놀 함량을 나타내며 DPPH 라디칼 소거능과 상관성 있는 결과를 확인하였다. 산화적 스트레스에 의한 간세포(HepG2) 및 폐세포(A549) 보호효과는 두 가지 추출물 모두 약 10%의 세포 보호능을 나타내며 다소 낮은 효과를 나타내었다. 추출물의 폐세포에 대한 독성율은 $100{\mu}g/mL$ 이하의 농도에서 독성을 나타내지 않았다. 본 연구 결과는 다정큼나무 및 참가시나무 추출물을 이용한 항산화 물질 개발을 위한 기초자료로 활용될 것이다.

Keywords

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