Journal of Plant Biotechnology

  • 제43권1호
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  • Pages.49-57
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  • 2016
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Identification and characterization of S-RNase genes in apple rootstock and the diversity of S-RNases in Malus species

  • Kim, Hoy-Taek (Department of Horticulture, Sunchon National University) ;
  • Moriya, Shigeki (Apple Research Station, NARO Institute of Fruit Tree Science) ;
  • Okada, Kazuma (Apple Research Station, NARO Institute of Fruit Tree Science) ;
  • Abe, Kazuyuki (Apple Research Station, NARO Institute of Fruit Tree Science) ;
  • Park, Jong-In (Department of Horticulture, Sunchon National University) ;
  • Yamamoto, Toshiya (NARO Institute of Fruit Tree Science) ;
  • Nou, Ill-Sup (Department of Horticulture, Sunchon National University)
  • 투고 : 2016.03.14
  • 심사 : 2016.03.15
  • 발행 : 2016.03.31
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초록

We isolated and confirmed two S-RNases, denoted as mpS1 and mpS2, from apple rootstock 'Marubakaido' (Malus prunifolia Borkh. Var. ringo Asami). These S-RNases contained and conserved five cysteine residues and two histidine residues, which are essential for RNase activity. The mpS1 showed high similarity to S5 (99.1%) of Malus spectabilis, whereas the mpS2 showed 99.5% nucleotide sequence similarity to S26 of (Malus ${\times}$ domestica) and 99.6% to S35 of (Malus sieversii) when compared with reported S-RNases. In amino acid sequences, the mpS1-RNase was almost similar to the S5-RNase of Malus spectabilis, and the mpS2-RNase was similar to the S35 of Malus sieversii, with only one bp being different from the S26-RNase of Malus ${\times}$ domestica. The 57 S-RNases of Malus species were renamed and rearranged containing the new S-RNases, as mprpS35 (mpS2) and mprpS57 (mpS1), for determining S-genotypes and identifying new alleles from apple species (Malus spp.).

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피인용 문헌

  1. Characterization of 25 full-length S-RNase alleles, including flanking regions, from a pool of resequenced apple cultivars vol.97, pp.3, 2018, https://doi.org/10.1007/s11103-018-0741-x