Journal of Plant Biotechnology

  • 제43권1호
  • /
  • Pages.37-48
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  • 2016
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Transcriptional profiles of Rhizobium vitis-inoculated and salicylic acid-treated 'Tamnara' grapevines based on microarray analysis

  • Choi, Youn Jung (National Institute for Horticultural and Herbal Science) ;
  • Yun, Hae Keun (Department of Horticulture and Life Science, Yeungnam University)
  • 투고 : 2016.03.02
  • 심사 : 2016.03.09
  • 발행 : 2016.03.31
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초록

The transcriptional profiles of 'Tamnara' grapevine (Vitis labruscana L.) to Rhizobium vitis were determined using 12,000 gene oligonucleotide microarray chips constructed with 6,776 unigenes based on the EST sequencing. Among them, 95 clones were up-regulated more than three times and 90 were down-regulated more than 5-times in the R. vitis-inoculated grapevines relative to the control vines. Treatment of salicylic acid showed that 337 clones were upregulated and 52 clones were down regulated in grapevines. Microarray analysis, reverse transcription-polymer chain reaction, and slot blot hybridization analysis revealed that 5, 14, and 64 clones were up-regulated and 10, 12, and 61 clones were down-regulated in wounded, salicylic acid-treated, and R. vitis-inoculated 'Tamnara' grapevine leaves, respectively. The expression patterns of ${\beta}$-1,3-glucanase, proline-rich protein, and lipoxygenase genes of 'Tamnara' moderately resistant to R. vitis were similar to those of resistant 'Concord' and 'Delaware' grapevines. However, chalcone synthase genes in 'Tamnara' grapevines showed similar expression patterns to susceptible grapevines 'Neomuscat' and 'Rizamat'. Further expression studies with various clones for each gene should be conducted to elucidate their roles in resistant responses against pathogens or other stimuli in grapevines. These results could provide better resources for understanding the mechanism of defense responses against crown gall disease and clues for identifying new genes that may play a role in defense against R. vitis in grapevines.

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참고문헌

  1. Anand A, Uppalapati SR, Ryu CM, Allen SN, Kang L, Tang Y, Mysore KS (2008) Salicylic acid and systemic acquired resistantce play a role in attenuating crown gall disease caused by Agrobacterium tumefaciens. Plant Physiol 146:703-715
  2. Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate, a practical and powerful approach to multiple testing. J Royal Stat Soc Ser B 57:289-300
  3. Burr TJ, Otten L (1999) Crown gall of grape: Biology and disease management. Annu Rev Phytopathol 37:53-80 https://doi.org/10.1146/annurev.phyto.37.1.53
  4. Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol 11:113-116 https://doi.org/10.1007/BF02670468
  5. Cheong YH, Chang H.S., Gupta R., Wang X, Zhu T, Luan S (2002) Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis. Plant Physiol 129:661-677 https://doi.org/10.1104/pp.002857
  6. Choi, YJ, Yun HK, Park KS, Rho JH, Jeong ST, Lee HJ, Jang HI (2008) Screening genes expressed by Rhizobium vitis inoculation and salicylic acid treatment in grapevines using GeneFishing. J Jpn Soc Hort Sci 77:137-142 https://doi.org/10.2503/jjshs1.77.137
  7. Choi, YJ, Yun HK, Park KS, Rho JH, Heo YY, Kim DW, Lee HJ (2010) Transcriptional profiling of ESTs responsive to Rhizobium vitis from 'Tamnara' grapevines (Vitis sp.). J Plant Physiol 167:1084-1092 https://doi.org/10.1016/j.jplph.2010.02.005
  8. Cramer GR, Urano K, Delrot S, Pezzotti M, Shinozaki K (2011) Effects of abiotic stress on plants: a systems biology perspective. BMC Plant Biol 11:163 https://doi.org/10.1186/1471-2229-11-163
  9. Creelman RA, Mullet JE (1997) Biosynthesis and action of jasmonates in plants. Annu Rev Plant Physiol Plant Mol Biol 48:355-381 https://doi.org/10.1146/annurev.arplant.48.1.355
  10. Deluc LG, Grimplet J, Wheatley MD, Tillett RL, Quilici DR, Osborne C, Schooley DA, Schlauch KA, Cushman JC, Cramer GR (2007) Transcriptomic and metabolite analyses of Cabernet Sauvignon grape berry development. BMC Genomics 8:429 https://doi.org/10.1186/1471-2164-8-429
  11. Dong X (1998) SA, JA, ethylene, and disease resistance in plants. Curr Opin Plant Biol 1:316-323 https://doi.org/10.1016/1369-5266(88)80053-0
  12. Espinoza C, Vega A, Medina C, Schlauch K, Cramer G, Arce-Johnson P (2007) Gene expression associated with compatible viral diseases in grapevine cultivars. Funct Integr Genomics 7:95-110 https://doi.org/10.1007/s10142-006-0031-6
  13. Figueiredo A, Fortes AM, Ferreira S, Sebastiana M, Choi YH, Sousa L, Acioli-Santos B, Pessoa F, Verpoorte R, Pais MS (2008) Transcriptional and metabolic profiling of grape (Vitis vinifera L.) leaves unravel possible innate resistance against pathogenic fungi. J Exp Bot 59:3371-3381 https://doi.org/10.1093/jxb/ern187
  14. Hur YY, Jung SM, Yun HK (2015) Current status and prospects of genomics and bioinformatics in grapes. J Plant Biotechnol 42:298-311 https://doi.org/10.5010/JPB.2015.42.4.298
  15. Kandler O, Hopf H (1984) Biosynthesis of oligosaccharides in vascular plants, p. 115-131. In DH Lewis, (ed.). Storage carbohydrates in vascular plants. Cambridge Univ. Press, Cambridge
  16. Keller F, Pharr D (1996) Metabolism of carbohydrates in sinks and sources: Galactosyl-sucrose oligosaccharides, p. 157-183. In E. Zamski, A.A. Schaffer, (eds.). Photoassimilate distribution in plants and crops: Source-sink relationships. Marcel Dekker, New York, USA
  17. Kerr A (1980) Biological control of crown gall through production of agrocin 84. Plant Dis 64:28-30
  18. Kunkel BN, Brooks DM (2002) Crosstalk between signaling pathways in pathogen defense. Curr Opin Plant Biol 5:325-331 https://doi.org/10.1016/S1369-5266(02)00275-3
  19. Lin H, Doddapaneni H, Takahashi T, Walker MA (2007) Comparative analysis of ESTs involved in grape responses to Xylella fastidiosa infection. BMC Plant Biol 7:8 https://doi.org/10.1186/1471-2229-7-8
  20. Malamy J, Carr JP, Klessig D, Raskin I (1990) Salicylic acid: A likely endogenous signal in the resistance response of tobacco to viral infection. Science 250:1002-1004 https://doi.org/10.1126/science.250.4983.1002
  21. Maleck K, Dietrich RA (1999) Defense on multiple fronts: How do plants cope with diverse enemies? Trends Plant Sci 4:215-219. https://doi.org/10.1016/S1360-1385(99)01415-6
  22. Meyers BC, Galbraith DW, Nelson T, Agrawal V (2004) Methods for transcriptional profiling in plants. Be fruitful and replicate. Plant Physiol 135:637-652 https://doi.org/10.1104/pp.104.040840
  23. Nishizawa A, Yabuta Y, Shigeoka S (2008) Galactinol and raffinose constitute a novel function to protect plants from oxidative damage. Plant Physiol 147:1251-1263 https://doi.org/10.1104/pp.108.122465
  24. Park KH, Jeong KS, Cha JS (2000) Incidence of severe crown gall disease on tetraploid cultivars of grape in Korea. Plant Pathol J 16:290-293
  25. Park KS, Yun HK, Suh HS, Jeong SB, Cho HM (2004) Breeding of early season grape cultivar 'Tamnara' grapevines (Vitis hybrid) with high quality and disease resistance. Korean J Hort Sci Technol 22:458-461
  26. Peterbauer T, Richter A (2001) Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds. Seed Sci Res 11:185-197
  27. Peterbauer T, Mach L, Mucha J, Richter A (2000) Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis. Planta 215:839-849
  28. Roh JH, Yun HK, Do KR, Park KS, Choi YJ (2005) Histochemical changes by Agrobacterium vitis infection in grapevines. J Korean Soc Hort Sci 46:260-263
  29. Shah J (2003) The salicylic acid loop in plant defense. Curr Opin Plant Biol 6:365-371 https://doi.org/10.1016/S1369-5266(03)00058-X
  30. Schulze A, Downward J (2001) Navigating gene expression using microarrays: A technology review. Nature 3:E190-195
  31. Stears RL, Martinsky T, Schena M (2003) Trends in microarray analysis. Nature 9:140-145
  32. Stover EW, Swartz HJ, Burr TJ (1997) Crown gall formation in a diverse collection of Vitis genotypes inoculated with Agrobacterium vitis. Am J Enol Vitic 48:26-32
  33. Terrier N, Glissant D, Grimplet J, Barrieu F, Abbal P, Couture C, Ageorges A, Atanassova R, Leon C, Renaudin JP, et al. (2005) Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development. Planta 222:832-847 https://doi.org/10.1007/s00425-005-0017-y
  34. The Franch-Italian Public Consortium for Grapevine (2007) The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature 449:463-467 https://doi.org/10.1038/nature06148
  35. Van Gelder RN, von Zastrow ME, Yool A, Dement WC, Barchas JD, Eberwine JH (1990) Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc Natl Acad Sci USA 87:1663-1667 https://doi.org/10.1073/pnas.87.5.1663
  36. Waters DL, Holton TA, Ablett EM, Lee LS, Henry RJ (2005) cDNA microarray analysis of developing grape (Vitis vinifera cv. Shiraz) berry skin. Funct Integr Genomics 5:40-58 https://doi.org/10.1007/s10142-004-0124-z
  37. Yun HK, Rho JH, Park KS, Cha JS, Jeong SB (2003) Screening system for crown gall resistance by pathogen inoculation in grapes. Korean J Hortic Sci Technol 21:325-328