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http://dx.doi.org/10.5010/JPB.2016.43.1.49

Identification and characterization of S-RNase genes in apple rootstock and the diversity of S-RNases in Malus species  

Kim, Hoy-Taek (Department of Horticulture, Sunchon National University)
Moriya, Shigeki (Apple Research Station, NARO Institute of Fruit Tree Science)
Okada, Kazuma (Apple Research Station, NARO Institute of Fruit Tree Science)
Abe, Kazuyuki (Apple Research Station, NARO Institute of Fruit Tree Science)
Park, Jong-In (Department of Horticulture, Sunchon National University)
Yamamoto, Toshiya (NARO Institute of Fruit Tree Science)
Nou, Ill-Sup (Department of Horticulture, Sunchon National University)
Publication Information
Journal of Plant Biotechnology / v.43, no.1, 2016 , pp. 49-57 More about this Journal
Abstract
We isolated and confirmed two S-RNases, denoted as mpS1 and mpS2, from apple rootstock 'Marubakaido' (Malus prunifolia Borkh. Var. ringo Asami). These S-RNases contained and conserved five cysteine residues and two histidine residues, which are essential for RNase activity. The mpS1 showed high similarity to S5 (99.1%) of Malus spectabilis, whereas the mpS2 showed 99.5% nucleotide sequence similarity to S26 of (Malus ${\times}$ domestica) and 99.6% to S35 of (Malus sieversii) when compared with reported S-RNases. In amino acid sequences, the mpS1-RNase was almost similar to the S5-RNase of Malus spectabilis, and the mpS2-RNase was similar to the S35 of Malus sieversii, with only one bp being different from the S26-RNase of Malus ${\times}$ domestica. The 57 S-RNases of Malus species were renamed and rearranged containing the new S-RNases, as mprpS35 (mpS2) and mprpS57 (mpS1), for determining S-genotypes and identifying new alleles from apple species (Malus spp.).
Keywords
Apple; Malus prunifolia; Marubakaido; S-RNase; Self-incompatibility;
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