Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry

  • Kim, Jia (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Song, Young-Sang (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Min, Kyung-San (Department of Conservative Dentistry, School of Dentistry, Chonbuk National University) ;
  • Kim, Sun-Hun (Research Center for Biomineralization Disorders, Chonnam National University) ;
  • Koh, Jeong-Tae (Research Center for Biomineralization Disorders, Chonnam National University) ;
  • Lee, Bin-Na (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Chang, Hoon-Sang (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Hwang, In-Nam (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Oh, Won-Mann (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Hwang, Yun-Chan (Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University)
  • Received : 2015.08.13
  • Accepted : 2015.11.09
  • Published : 2016.02.29
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Abstract

Objectives: The purpose of this study was to assess the ability of two new calcium silicate-based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA). Materials and Methods: Eighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP). Results: On micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP. Conclusions: Our results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.

Keywords

References

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