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Alternaria Leaf Spot Caused by Alternaria mali on Black Chokeberry in Korea

Alternaria mali에 의한 아로니아 점무늬낙엽병

  • Hahm, Soo-Sang (Agricultural Environment Research Division, Chungnam Agricultural Research and Extension Services) ;
  • Kwon, Mi-Kyung (Agricultural Environment Research Division, Chungnam Agricultural Research and Extension Services) ;
  • Kim, Byung-Ryun (Agricultural Environment Research Division, Chungnam Agricultural Research and Extension Services) ;
  • Han, Kwang-Seop (Agricultural Environment Research Division, Chungnam Agricultural Research and Extension Services) ;
  • Nam, Yun-Gyu (Agricultural Environment Research Division, Chungnam Agricultural Research and Extension Services)
  • 함수상 (충남농업기술원 농업환경과) ;
  • 권미경 (충남농업기술원 농업환경과) ;
  • 김병련 (충남농업기술원 농업환경과) ;
  • 한광섭 (충남농업기술원 농업환경과) ;
  • 남윤규 (충남농업기술원 농업환경과)
  • Received : 2015.10.22
  • Accepted : 2016.02.22
  • Published : 2016.03.31

Abstract

In early June 2014, leaf spot symptoms were observed on black chokeberry (Aronia melanocarpa) in Yesan-gun and Geumsan-gun, Chungcheongnam-do in Korea. The initial symptoms on leave surfaces were brown small-circular spots with a yellow halo lesion, and gradually the small spots were fused, all of infected leaves dropped eventually. A fungus were isolated from the initial lesion, and cultured on potato dextrose agar. Colony color on upper surface of plate varied from olive gray to charcoal gray. Size of conidia mostly extend to $19-50{\times}5-9{\mu}m$ in nature and $20-59{\times}8-13{\mu}m$ in culture, with 3-8 transverse septa and usually no longitudinal septum or only 1 longitudinal septum in 1-3 of the transverse compartments, and also have a short or long beak. Pathogenicity was investigated using wounded or unwounded black chokeberry and apple leaves. After 7 days of inoculation, leaf spots were similar to the symptoms naturally occurred in the field. On the basis of mycological characteristics, pathogenicity, and ITS rDNA sequence analysis, this fungus was identified as Alternaria mali. This is the first report of Alternaria leaf spot on black chokeberry caused by A. mali in Korea.

2014년 6월 초 충남 예산과 금산의 농가포장에서 재배 중인 아로니아에서 점무늬낙엽병으로 의심되는 증상이 관찰되었다. 초기에는 잎에 갈색의 작은 점무늬가 생기고 이것이 확대되면서 둥근 겹무늬를 형성하고, 암갈색으로 변하였다. 그리고 병세가 심해지면 잎 전체에 병반이 확대되면서 떨어지는 증상이 관찰되었다. 병든 아로니아 잎에서 분리한 병원균은 PDA에 배양하였을 때 균체는 올리브 회색과 진한 회색으로 다양한 색을 나타내었다. 포장에서 감염된 잎과 PDA에서 분리한 분생포자의 크기는 각각 $19-50{\times}5-9{\mu}m$, $20-59{\times}8-13{\mu}m$이고, 길거나 짧은 부리를 가지며, 분생자경의 끝에 1-3개의 분생포자가 형성되었으며, 3-8개의 횡경막을 보이고 종경막은 없거나 1-3번째 횡경막 내에 1개 형성되었다. 병원성 검정 결과, 접종 7일 후 아로니아와 사과 모두에서 자연발생한 병징과 동일하게 관찰되었다. 이상 병원균의 형태적 특성, 병원성 검정과 ITS rDNA 염기서열 비교분석 결과를 토대로 아로니아에서 분리한 병원균은 A. mali로 동정하였고, 이 병을 아로니아 점무늬낙엽병으로 명명하였다.

Keywords

References

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  1. First Report of Leaf Spot Caused byAlternaria tenuissimaon Black Chokeberry (Aronia melanocarpa) in Korea vol.44, pp.3, 2016, https://doi.org/10.5941/MYCO.2016.44.3.187
  2. Identification and Characterization of Pseudocercospora pyricola Causing Leaf Spots on Aronia melanocarpa vol.45, pp.1, 2017, https://doi.org/10.5941/MYCO.2017.45.1.39