Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Twig Blight on Chinese Magnolia Vain Caused by Botryosphaeria dothidea in Korea

Botryosphaeria dothidea에 의한 오미자 줄기마름병

  • Park, Sangkyu (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Seung-Han (Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Lee, Seung-Yeol (School of Applied Biosciences, Kyungpook National University) ;
  • Back, Chang-Gi (School of Applied Biosciences, Kyungpook National University) ;
  • Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University) ;
  • Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University)
  • 박상규 (경북대학교 응용생명과학부) ;
  • 김승한 (경상북도농업기술원) ;
  • 이승열 (경북대학교 응용생명과학부) ;
  • 백창기 (경북대학교 응용생명과학부) ;
  • 강인규 (경북대학교 원예과학과) ;
  • 정희영 (경북대학교 응용생명과학부)
  • Received : 2015.12.17
  • Accepted : 2016.03.04
  • Published : 2016.03.31
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Abstract

The twig blight symptoms were observed in Chinese magnolia vine (Schisandra chinensis) at Mungyeong city, Gyeongbuk province, Korea in June 2015. The typical symptoms of infected plant were shriveled and wilted in leaves which led to blight resulted in death. Based on the morphological characteristics, the isolate was suspected as Botryosphaeria sp. Inoculation of isolated pathogen was performed to identify its pathogenicity according to Koch's postulates. Re-isolated fungi from the inoculated stem was showed same morphological characteristics with original pathogen. Phylogenetic analysis was performed using combined sequence of rDNA internal transcribed spacer region, EF1-${\alpha}$ and ${\beta}$-tubulin gene. The isolated pathogen was identified to the B. dothidea by phylogenetic analysis. This is the first report of twig blight on S. chinensis caused by B. dothidea in Korea.

2015년 6월에 경북 문경시에서 재배하는 오미자에서 줄기마름증상이 발생하였다. 발병 초기에는 잎의 선단 부위부터 위축되면서 고사현상이 발생하였고, 병이 진행됨에 따라 줄기를 거쳐 식물체 전체가 고사하였으며 주변으로 전염되었다. 감염된 줄기에서 병원균을 분리배양하여 배양적, 형태적 특징을 관찰한 결과 해당 병원균은 Botryosphaeria속에 속하는 것으로 나타났으며, 인공접종에 의한 병원성 검정에서도 동일한 병징을 나타내었다. 병원균의 정확한 동정을 위해 ITS 영역과 ${\beta}$-tubulin, EF1-${\alpha}$ 유전자의 염기서열을 복합적으로 사용한 염기서열 분석을 수행하였으며, 분석 결과 오미자의 줄기마름 증상을 일으키는 원인균은 B. dothidea로 동정되었다. 지금까지 국내에서 B. dothidea에 의한 오미자의 병이 보고되지 않았기에 본 연구를 통해 국내 처음으로 B. dothidea에 의한 오미자 줄기마름병을 보고한다.

Keywords

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