농약과학회지 (The Korean Journal of Pesticide Science)

  • 제20권1호
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  • Pages.30-34
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  • 2016
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
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식물정유의 당근뿌리혹선충(Meloidogyne hapla)에 대한 살선충활성 및 방향성성분 분석

Chemical Compositions and Nematicidal Activities of Essential Oils on Meloidogyne hapla (Nematoda: Tylenchida) Under Laboratory Conditions

  • 전주현 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 고형래 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 김세종 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이재국 (농촌진흥청 국립농업과학원 작물보호과)
  • Jeon, Ju-Hyun (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Ko, Hyoung-Rai (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Se-Jong (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Jae-Kook (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA)
  • 투고 : 2015.11.20
  • 심사 : 2016.02.28
  • 발행 : 2016.03.31
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초록

본 연구는 뿌리혹선충의 친환경적 방제를 위하여 9종의 식물정유를 선발하여 당근뿌리혹선충 2령기 유충과 알에 대한 살선충 및 부화억제 효과를 검정하였다. 당근뿌리혹선충의 2령기 유충에 대해 $125{\mu}g/mL$로 검정한 결과, Alpinia galanga 정유가 100% 살선충활성을 나타냈고, Carum carbi (22.3%), Eugenia caryophyllata (9.4%), Cinnamonum zeylanicum (7.2%), Mentha pulegium (2.4%) 및 Foeniculum vulgare (2.1%) 순으로 활성을 나타내었다. 이들 중 A. galanga 정유를 이용하여 당근뿌리혹선충 알의 부화억제 활성을 검정한 결과, $500{\mu}g/mL$ 농도로 처리 후 7, 14, 21 일째까지 낮은 부화 활성을 나타내었다. 우수한 활성을 보인 A. galanga 정유의 활성 성분을 분석한 결과 methyl cinnamate 함유율이 가장 높았으며, 1,8-cineole, ${\beta}$-pinene, ${\alpha}$-pinene, p-cymene이 주요 방향성 물질로 구성되어 있음을 확인할 수 있었다. 따라서 본 연구에서 선발한 A. galanga 정유가 당근뿌리혹선충의 친환경적 방제수단으로 활용가능성이 있을 것으로 생각된다.

To evaluate the efficacy of natural nematicides for the control of root-knot nematode in strawberry greenhouses, commercial essential oils were examined by 24-well culture plate bioassay for their nematicidal activities against second-stage juveniles and eggs of Meloidogyne hapla. Based on the mortality of M. hapla juveniles at a concentration of $125{\mu}g/mL$, the most active essential oil was Alpinia galanga (100%), followed by Carum carbi (22.3%), Eugenia caryophyllata (9.4%), Cinnamonum zeylanicum (7.2%), Mentha pulegium (2.4%), and Foeniculum vulgare (2.1%). Moreover, A. galanga significantly reduced hatching at 7, 14, and 21 days after treatment. The volatile constituents identified in the A. galangal oil were methyl cinnamate (87.4%), 1,8-cineole (4.4%), ${\beta}$-pinene (2.5%), ${\alpha}$-pinene (2.2%), and p-cymene (1.1%), as major constituents. Results of this study show that A. galangal essential oil and its major constituents may serve as an environmental friendly agent of a promising natural nematicide to control Meloidogyne spp.

키워드

참고문헌

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피인용 문헌

  1. Phytochemical biopesticides: some recent developments vol.16, pp.5, 2017, https://doi.org/10.1007/s11101-017-9512-6
  2. Nematicidal screening of essential oils and potent toxicity of Dysphania ambrosioides essential oil against Meloidogyne incognita in vitro and in vivo vol.167, pp.7, 2016, https://doi.org/10.1111/jph.12803
  3. Biological activities of essential oils and lipopeptides applied to control plant pests and diseases: a review vol.67, pp.2, 2016, https://doi.org/10.1080/09670874.2019.1707327