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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Development of Genome Engineering Tools for Metabolic Engineering of Butanol-producing Clostridium Species

Butanol 생합성 Clostridium 속 미생물 대사공학용 게놈 편집 도구 개발

  • Woo, Ji Eun (Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Gyeongsang National University) ;
  • Kim, Minji (Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Gyeongsang National University) ;
  • Lee, Ji Won (Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Gyeongsang National University) ;
  • Seo, Hyo Joo (Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Gyeongsang National University) ;
  • Lee, Sang Yup (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus program), Bioinformatics Research Center, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, KAIST) ;
  • Jang, Yu-Sin (Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Gyeongsang National University)
  • 우지은 (경상대학교 농업생명과학연구원) ;
  • 김민지 (경상대학교 농업생명과학연구원) ;
  • 이지원 (경상대학교 농업생명과학연구원) ;
  • 서효주 (경상대학교 농업생명과학연구원) ;
  • 이상엽 (한국과학기술원 생명화학공학과) ;
  • 장유신 (경상대학교 농업생명과학연구원)
  • Received : 2016.11.22
  • Accepted : 2016.12.05
  • Published : 2016.12.31
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Abstract

Global warming caused from the heavy consumption of fossil fuel is one of the biggest problems to be solved. Biofuel has been gained more attention as an alternative to reduce the consumption of fossil fuel. Recently, butanol produced from the genus Clostridium has been considered as one of the promising alternatives for gasoline, fossil based fuel. Nevertheless, the lack of the genome-engineering tools for the genus Clostridium is the major hurdle for the economic production of butanol. More recently, genome engineering tools have been developed for metabolic engineering of butanol-producing Clostridium species, which includes genome scale network model and genome editing tools on the basis of mobile group II introns and CRISPR/Cas system. In this study, the genome engineering tools for butanol-producing Clostridium species have been reviewed with a brief future perspective.

Keywords

References

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