Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Prevalence of honeybee (Apis mellifera) disease in Daejeon

대전광역시 양봉농가의 꿀벌질병 감염률 조사

  • Kim, Young-ju (Daejeon Metropolitan City Institute Health and Envionment) ;
  • Kim, Jong-ho (Daejeon Metropolitan City Institute Health and Envionment) ;
  • Oh, Yoon-hee (Daejeon Metropolitan City Institute Health and Envionment) ;
  • Lee, Sang-joon (Daejeon Metropolitan City Institute Health and Envionment) ;
  • Song, Sun-kyong (Daejeon Metropolitan City Institute Health and Envionment) ;
  • Joung, Eun-young (Daejeon Metropolitan City Institute Health and Envionment) ;
  • Lee, Sang-joon (Daejeon Metropolitan City Institute Health and Envionment) ;
  • Lee, Seok-ju (Daejeon Metropolitan City Institute Health and Envionment) ;
  • Moon, Byeong-cheon (Daejeon Metropolitan City Institute Health and Envionment)
  • 김영주 (대전광역시보건환경연구원) ;
  • 김종호 (대전광역시보건환경연구원) ;
  • 오윤희 (대전광역시보건환경연구원) ;
  • 이상준 (대전광역시보건환경연구원) ;
  • 송선경 (대전광역시보건환경연구원) ;
  • 정은영 (대전광역시보건환경연구원) ;
  • 이상준 (대전광역시보건환경연구원) ;
  • 이석주 (대전광역시보건환경연구원) ;
  • 문병천 (대전광역시보건환경연구원)
  • Received : 2016.11.28
  • Accepted : 2016.12.26
  • Published : 2016.12.30
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Abstract

This study was conducted to investigate the prevalence of honey bee (Apis mellifera) disease in Daejeon. From May to September in 2014, 63 samples were collected from 63 apiculture farms in the regions and reverse transcriptase-polymerase chain reaction (RT-PCR) and polymerase chain reaction (PCR) was conducted. A total of 11 infectious pathogens, including 6 virus, 2 bacteria, 2 fungi, and 1 parasite, were investigated in honeybee colonies suffering from symptom of sudden collapse, depopulation or paralysis. The infectious pathogens and infection rates among 63 honeybee colonies detected were as follows: sacbrood virus (12.7%), chronic bee paralysis virus (1.6%), stonebrood (11.1%), American foulbrood (19.0%), European foulbrood (6.3%), respectively. The result indicate that foul-brood was most prevalent disease in apiculture farms in Daejeon area.

Keywords

References

  1. Allen MF, Ball BV. 1996. The incidence and world distribution of the honey bee viruses. Bee World. 77: 141-162. https://doi.org/10.1080/0005772X.1996.11099306
  2. Benjeddou M. Leat N, Allsopp M, Davison S. 2001. Detection of Acute Bee Paralysis Virus and Black Queen Cell Virus from honeybees by Reverse Transcriptase PCR. Appl Env Microbiol 67: 2384-2387 https://doi.org/10.1128/AEM.67.5.2384-2387.2001
  3. Gilliam M. 1997. Identification and roles of non-pathogenic microflora associated with honet bees. FEMS Microbiol. Lett. 155: 1-10 https://doi.org/10.1016/S0378-1097(97)00337-6
  4. Grabensteiner W, Ritter W, Carter MJ, Davison S, Pechacker H, Kolodziejek J, Boecking O, Derakhshifar I, Moosbeckhofer R, Licek E, Nowotny N. 2001. Sacbrood virus of the honeybee (Apis mellifera): rapid identification and phylogenetic analysis using reverse trascription-PCR. Clin Diagn Lab Immunol 8: 93-104.
  5. Ha JS, Lee HM, Kim DS, Lim YK, Yoon BS. 2005. A PCR detection method of Melissococcus pluton for rapid identification of European foulbrood. Korean J Apiculture 20: 9-18.
  6. Jeon DM, Kim SH, Yook SY, Yeam NH, Do JY, Song SY, Heo EJ, Sin CH. 2013. Prevalence of honeybee (Apis mellifera) disease in Cheonan-Asan areas, Korea. Korea. Korean J Vet Serv 36: 147-150. https://doi.org/10.7853/kjvs.2013.36.2.147
  7. Kim HK, Choi YS, Lee ML, Lee MY, Lee KG, Ahn NH. 2008. Detection of sacbrood virus(SBV) from the honeybee in Korea. Korean J Apiculture 23: 103-109.
  8. Lee HM, Ha JS, Jo YH, Nam SH, Yoon BS. 2004. PCR detection method of Ascosphera apis, Aspergillus flavus for rapid identification of fungal disease in honeybee. Korean J apiculture 19: 139-148.
  9. Lee HM, Lee DB, Han SH, Nam SH, Lim YK, Yoon BS. 2005. Rapid identification of Ascosphera apis causing chalkbrood disease in honeybee by real-time PCR. Korean J Apiculture 20: 109-116.
  10. Liu X, ZhangY, Yan X, Han R. 2010. Prevention of Chinese sacbrood virus infection in Apis cerana using RNA interference. Curr Microbiol 61: 422-428. https://doi.org/10.1007/s00284-010-9633-2
  11. Oldroyd BP. 2007. What's killing American honey bees? PloSBiol 5: 1195-1199.
  12. Olofsson TC, Vasquez A. 2008. Detection and identification of a novel lactic acid bacterial flora within the honey stomach of the honeybee Apis mellifera. Curr. Microbiol. 47: 356-363.
  13. Ouh IO, Do JC, Jeong TN, Cho MH, Kwak DM. 2013. Molecular detection of infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province, Korea. Korean J Vet Serv 36: 37-44. https://doi.org/10.7853/kjvs.2013.36.1.37
  14. Ribiere M, Triboulot C, Mathieu L, Aurieres C, Faucon JP, Pepin M. 2002. Molecular diagnostic of choronic bee paralysis virus infection. Apidologie 33: 339-351. https://doi.org/10.1051/apido:2002020
  15. Stoltz D, Shen XR, Boggis C, Sisson G. 1995. Molecular diagnosis of Kashmir bee virus infection. J Api Res 34: 153-160. https://doi.org/10.1080/00218839.1995.11100900
  16. Yoo MS, Lee DW, Kim IW, Kim DS, Kwon SH, Lim YG, Yoon BS. 2007. Identification of black queen cell virus from the honeybee in Korea. korean j Apiculture 22: 43-52.
  17. Yoo MS, Yoon BS. 2009. Incidence of honeybee disease in korea 2009. Korea J Apiculture 24: 273-278.

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