Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Genetic sequence and phylogenetic analysis of spike genes of Porcine epidemic diarrhea virus (PEDV) in Chung-Nam area

충남지역 돼지유행성설사 바이러스 Spike 유전자 염기서열 및 계통분석

  • Park, Hyo-Seon (Veterinary Epidemiology Division, Chungnam Veterinary Research Institute) ;
  • Yook, Sim-Yong (Veterinary Epidemiology Division, Chungnam Veterinary Research Institute) ;
  • Jeon, Dong-Min (Veterinary Epidemiology Division, Chungnam Veterinary Research Institute) ;
  • Lee, Jin-Ju (Veterinary Epidemiology Division, Chungnam Veterinary Research Institute) ;
  • Shin, Chang-Ho (Veterinary Epidemiology Division, Chungnam Veterinary Research Institute)
  • 박효선 (충청남도가축위생연구소 역학조사과) ;
  • 육심용 (충청남도가축위생연구소 역학조사과) ;
  • 전동민 (충청남도가축위생연구소 역학조사과) ;
  • 이진주 (충청남도가축위생연구소 역학조사과) ;
  • 신창호 (충청남도가축위생연구소 역학조사과)
  • Received : 2016.12.13
  • Accepted : 2016.12.26
  • Published : 2016.12.30
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Abstract

Porcine epidemic diarrhea virus (PEDV) causes an acute and lethal watery diarrhea in piglets that is great economic losses to the swine country worldwide. The spike (S) glycoprotein is an important determinant for PEDV biological properties. In the present study, we determined the full-length S gene sequences of five Chung-nam PEDV field isolates collected in 2016. The S gene was amplified by RT-PCR, purificated, sequenced, analyzed and then compared with published sequences of other PEDV strains. 5 field strains share 98.5%~99.9% homologies with each other at the nucleotide sequence level and 96.7%~99.9% homologies with each other at the amino acids sequence level. Most field strains have nucleotide insertions, deletions and mutation regions, and show lower homologies (93.1~93.8%) with classical and vaccine strains, however higher homologies (99.1%~99.5%) with US PEDV isolates in 2013. By phylogenetic tree analysis based on nucleotide sequence, five PEDV field isolates were clustered into Genogroup 2b but differ genetically from the vaccine strains (SM-98 and DR-13).

Keywords

References

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