Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Isolation and Evaluation of Anti-oxidative Constituents from the Extracts of Ficus erecta var. sieboldii King Leaves

좁은잎천선과 잎 추출물 유래 항산화 활성 성분의 동정 및 효능 확인

  • Park, Sung Hwan (Department of Chemistry and Cosmetics, Jeju National University) ;
  • Kim, Jung Eun (Department of Chemistry and Cosmetics, Jeju National University) ;
  • Yeum, Hyun Sook (Department of Chemistry and Cosmetics, Jeju National University) ;
  • Lee, Nam Ho (Department of Chemistry and Cosmetics, Jeju National University)
  • 박성환 (제주대학교 화학.코스메틱스학과) ;
  • 김정은 (제주대학교 화학.코스메틱스학과) ;
  • 염현숙 (제주대학교 화학.코스메틱스학과) ;
  • 이남호 (제주대학교 화학.코스메틱스학과)
  • Received : 2016.09.07
  • Accepted : 2016.12.09
  • Published : 2016.12.30
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Abstract

In this study, we investigated identification of anti-oxidative constituents from Ficus erecta var. sieboldii King (F. erecta) leaves. DPPH and $ABTS^+$ radical scavenging activities were screened for the ethanol extract and solvent fractions, and ethyl acetate fraction showed the most potent scavenging activities. Five constituents were isolated from the ethyl acetate fraction of F. erecta leaves; monoolein (1), oleic acid (2), lutein (3), afzelechin (4), catechin (5). The chemical structures of the isolated compounds were elucidated based on the spectroscopic data including NMR spectra, as well as comparison of the data to the literature values. As far as we know, all of the compounds 1 ~ 5 were isolated for the first time from this plant. Studies on DPPH and $ABTS^+$ radical scavenging activities were conducted for the isolated compounds. Among them, afzelechin (4) and catechin (5) showed strong DPPH and $ABTS^+$ radical scavenging activities, whose activities were comparable to a positive control vitamin C. Also, the content of catechin isolated from this plant was determined by HPLC and it was about 3.8 mg/g for the 70% ethanol extract and 20.8 mg/g for the ethyl acetate fraction. From these results, F. erecta leaves extract could be potentially applicable as anti-oxidant ingredients in cosmetic industries.

본 연구에서는 좁은잎천선과 잎 추출물 및 극성별 용매 분획물의 항산화 활성을 검색하고 유효성분을 분리하여 화학구조를 규명하였다. 좁은잎천선과 잎 추출물 및 용매 분획물의 DPPH 및 $ABTS^+$ 라디칼 소거 활성을 측정한 결과 에틸아세테이트 분획물에서 우수한 라디칼 소거 활성을 확인하였다. 활성이 좋은 에틸아세테이트 분획물에서 유효성분을 찾고자 vacuum liquid chromatography (VLC), silica gel column chromatography를 실시하였으며, 분리된 화합물은 1H 및 13C NMR 데이터 분석 및 문헌치 비교를 통하여 총 5개의 화합물을 동정하였다. 분리된 화합물은 monoolein (1), oleic acid (2), lutein (3), afzelechin (4), catechin (5)으로 확인되었으며 이들은 모두 좁은잎천선과에서 처음으로 분리된 화합물이다. 분리된 화합물에 대한 DPPH 및 $ABTS^+$ 라디칼 소거 활성 실험 결과 afzelechin (4) 및 catechin (5)에서 활성이 우수하게 나타났으며 특히 catechin (5)의 경우 대조군인 비타민 C보다 더 좋은 라디칼 소거 활성이 있음을 확인하였다. 또한 HPLC 분석을 통해 좁은잎천선과 잎에서 분리된 catechin의 함량을 확인한 결과 추출물에서 3.8 mg/g, 에틸아세테이트 분획물에서 20.8 mg/g이 함유되어 있는 것으로 확인되었다. 이상의 연구 결과로부터 좁은잎천선과 잎을 이용한 천연 항산화 소재로의 개발이 가능할 것이라 사료된다.

Keywords

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