DOI QR코드

DOI QR Code

Streptomyces sp. A75와 A501 균주의 인삼 잘록병에 대한 방제효과

Control of Ginseng Damping-off by Streptomyces sp. A75 and A501

  • 이상엽 (국립농업과학원 농업미생물과) ;
  • 송재경 (국립농업과학원 농업미생물과) ;
  • 윤봉식 (전북대학교 환경생명자원대학 생명공학부) ;
  • 박경훈 (국립원예특작과학원 인삼과) ;
  • 김정준 (국립농업과학원 농업미생물과) ;
  • 한지희 (국립농업과학원 농업미생물과)
  • Lee, Sang Yeob (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Song, Jaekyeong (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Yun, Bong-Sik (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Park, Kyeong hun (Ginseng Research Division, National Institute of Horticultural and Herbal Science) ;
  • Kim, Jeong Jun (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Han, Ji Hee (Agricultural Microbiology Division, National Academy of Agricultural Science)
  • 투고 : 2016.09.07
  • Accepted : 2016.11.25
  • Published : 2016.12.30

Abstract

Streptomyces sp. A75와 A501 균주는 인삼 잘록병을 일으키는 R. solani와 Pythium sp.의 균사 생장을 억제하였다. 인삼 잘록병 R. solani에 대하여 Streptomyces sp. A75와 A501 균주의 배양액을 혼합한 50배 희석액에 종자 침지처리, 100배 희석액의 관주처리와 종자 침지처리(50배) + 관주처리(100배)는 81.3%, 84.8%와 32.2%의 방제 효과를 각각 나타내었다. 인삼 잘록병 Pythium sp.에 대하여 Streptomyces sp. A75와 A501 균주의 배양액을 혼합한 50배 희석액에 종자 침지처리, 100배 희석액의 관주처리와 종자 침지처리(50배) + 관주처리(100배)는 51.0%, 52.1%, 75.3%의 방제효과를 각각 나타내었다. 이들 결과에서 Streptomyces sp. A75와 A501 균주의 배양한 혼합액으로 종자 침지한 후 관주처리가 인삼 잘록병 발생을 효과적으로 감소시켰다.

Streptomyces sp. A75 and A501 inhibited the mycelial growth of pathogenic Rhizoctonia solani and Pythium sp., which cause the ginseng disease known as damping-off. Three methods were evaluated for the control of these pathogens, using a mixture of the culture broths from Streptomyces sp. A75 and A501. The methods tested were seed dipping with 50-fold diluted broth, drenching of soil with 100-fold diluted broth after sowing, and combined seed dipping and drenching. These methods reduced the incidence of ginseng damping-off caused by R. solani by 81.3%, 84.8%, and 32.2% and that caused by Pythium sp. by 51.0%, 52.1%, and 75.3%, respectively. Based on these results, the combination of seed dipping and soil drenching after sowing using a mixture of the culture broths from Streptomyces sp. A75 and A501 effectively reduced the incidence of damping-off in ginseng.

Keywords

References

  1. Li YB, Wang Y, Tang JP, Chen D, Wang SL. Neuroprotective effects of ginsenoside Rg1-induced neural stem cell transplantation on hypoxic-ischemic encephalopathy. Neural Regen Res 2015;10:753-9. https://doi.org/10.4103/1673-5374.156971
  2. Saba E, Jeon BR, Jeong DH, Lee K, Goo YK, Kim SH, Sung CK, Roh SS, Kim SD, Kim HK, et al. Black ginseng extract ameliorates hypercholesterolemia in rats. J Ginseng Res 2016;40:160-8. https://doi.org/10.1016/j.jgr.2015.07.003
  3. Gui QF, Xu ZR, Xu KY, Yang YM. The efficacy of ginsengrelated therapies in type 2 diabetes mellitus: an updated systematic review and meta-analysis. Medicine (Baltimore) 2016;95:e2584. https://doi.org/10.1097/MD.0000000000002584
  4. Jang KJ, Choi SH, Yu GJ, Hong SH, Chung YH, Kim CH, Yoon HM, Kim GY, Kim BW, Choi YH. Anti-inflammatory potential of total saponins derived from the roots of Panax ginseng in lipopolysaccharide-activated RAW 264.7 macrophages. Exp Ther Med 2016;11:1109-15. https://doi.org/10.3892/etm.2015.2965
  5. Lee DY, Jeong YT, Jeong SC, Lee MK, Min JW, Lee JW, Kim GS, Lee SE, Ahn YS, Kang HC, et al. Melanin biosynthesis inhibition effects of ginsenoside Rb2 isolated from Panax ginseng berry. J Microbiol Biotechnol 2015;25:2011-5. https://doi.org/10.4014/jmb.1505.05069
  6. The Korean Society of Plant Pathology. List of plant diseases in Korea, 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
  7. Agrios, GN. Plant pathology. 5th ed. Boston: Elsevier Academic Press; 2005.
  8. Cho DH, Cho HS, Shin JS, Park DW. Control of damping-off and ginseng stem fungus gnat of ginseng. In: KT&G Ginseng research report, 2007. p. 28-33.
  9. Hong SK. Survey of ginseng cultivation in Southern Korea. So-yeun (Central Monopoly Institute) 1964;6:27-44.
  10. Georgakopoulos DG, Fiddaman P, Leifert C, Malathrakis NE. Biological control of cucumber and sugar beet damping-off caused by Pythium ultimum with bacterial and fungal antagonists. J Appl Microbiol 2002;92:1078-86. https://doi.org/10.1046/j.1365-2672.2002.01658.x
  11. Howell CR, Stipanovic RD. Suppression of Pythium ultimuminduced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic, pyoluteorin. Phytopathology 1980;70:712-5. https://doi.org/10.1094/Phyto-70-712
  12. Zamanizadeh HR, Hatami N, Aminaee MM, Rakhshandehroo F. Application of biofungicides in control of damping disease off in greenhouse crops as a possible substitute to synthetic fungicides. Int J Environ Sci Technol (Tehran) 2011;8:129-36. https://doi.org/10.1007/BF03326202
  13. Kim JH, Choi YH, Kang SJ, Rhee IK, Joo GJ. Biocontrol of vegetables damping-off by Bacillus ehimensis YJ-37. Kor J Life Sci 2002;12:416-22. https://doi.org/10.5352/JLS.2002.12.4.416
  14. Joo GJ, Kim JH, Kang SJ. Isolation and antifungal activity of Bacillus ehimensis YJ-37 as antagonistic against vegetables damping-off fungi. Kor J Life Sci 2002;12:200-7. https://doi.org/10.5352/JLS.2002.12.2.200
  15. Jo EJ, Kang BG, Jang KS, Choi YH, Kim JC, Choi GJ. Control efficacy of serenade formulation against Rhizoctonia and Pythium damping-off diseases. Res Plant Dis 2014;20:201-5. https://doi.org/10.5423/RPD.2014.20.3.201
  16. Woo EE, Lee GS, Lee IK, Choi JE, Yun BS. Control of ginseng damping-off by Streptomyces sp. A3265. Kor J Mycol 2016;44:193-5.
  17. Lahdenpera ML. Streptomyces-a new tool for controlling plant diseases. Agro Food Ind Hi Tech 1991;2:25-7.
  18. Elson MK, Kelly JF, Nair MG. Influence of antifungal compounds from a soil-borne actinomycete on Fusarium spp. in asparagus. J Chem Ecol 1994; 20:2835-46. https://doi.org/10.1007/BF02098392
  19. Handelsman J, Stabb EV. Biocontrol of soilborne plant pathogens. Plant Cell 1996;8:1855-69. https://doi.org/10.1105/tpc.8.10.1855
  20. Song J, Lee SC, Kang JW, Baek HJ, Suh JW. Phylogenetic analysis of Streptomyces spp. isolated from potato scab lesions in Korea on the basis of 16S rRNA gene and 16S-23S rDNA internally transcribed spacer sequences. Int J Syst Evol Microbiol 2004;54:203-9. https://doi.org/10.1099/ijs.0.02624-0
  21. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716-21. https://doi.org/10.1099/ijs.0.038075-0
  22. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9. https://doi.org/10.1093/molbev/msr121
  23. Saintpierre D, Amir H, Pineau R, Sembiring L, Goodfellow M. Streptomyces yatensis sp. nov., a novel bioactive streptomycete isolated from a New-Caledonian ultramafic soil. Antonie Van Leeuwenhoek 2003;83:21-6. https://doi.org/10.1023/A:1022906325397
  24. Esnard J, Potter TL, Zuckerman BM. Streptomyces costaricanus sp. nov., isolated from nematode-suppressive soil. Int J Syst Bacteriol 1995;45:775-9. https://doi.org/10.1099/00207713-45-4-775